Experiment: Carbon source D-Glucose 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaiW and yaiY are separated by 58 nucleotides yaiY and b0380 are separated by 259 nucleotides b0380 and ddlA are separated by 23 nucleotides ddlA and yaiB are separated by 462 nucleotides yaiB and phoA are separated by 100 nucleotides
b0378: yaiW - predicted DNA-binding transcriptional regulator (NCBI), at 397,096 to 398,190
yaiW
b0379: yaiY - predicted inner membrane protein (NCBI), at 398,249 to 398,557
yaiY
b0380: b0380 - orf, hypothetical protein (VIMSS), at 398,817 to 399,029
b0380
b0381: ddlA - D-alanylalanine synthetase (NCBI), at 399,053 to 400,147
ddlA
b0382: yaiB - hypothetical protein (NCBI), at 400,610 to 400,870
yaiB
b0383: phoA - alkaline phosphatase (VIMSS), at 400,971 to 402,386
phoA
Position (kb) 399
400
401 Strain fitness (log2 ratio) -1
0
1
2 at 398.318 kb on - strand, within yaiY at 398.351 kb on + strand, within yaiY at 398.386 kb on - strand, within yaiY at 398.386 kb on - strand, within yaiY at 398.392 kb on - strand, within yaiY at 398.392 kb on - strand, within yaiY at 398.593 kb on - strand at 398.608 kb on + strand at 398.613 kb on - strand at 398.633 kb on - strand at 398.755 kb on + strand at 398.755 kb on + strand at 398.809 kb on + strand at 398.852 kb on + strand, within b0380 at 398.860 kb on - strand, within b0380 at 398.860 kb on - strand, within b0380 at 398.883 kb on + strand, within b0380 at 398.933 kb on + strand, within b0380 at 398.933 kb on + strand, within b0380 at 398.933 kb on + strand, within b0380 at 398.959 kb on - strand, within b0380 at 398.959 kb on - strand, within b0380 at 399.034 kb on + strand at 399.034 kb on + strand at 399.079 kb on + strand at 399.092 kb on - strand at 399.092 kb on - strand at 399.129 kb on + strand at 399.129 kb on + strand at 399.129 kb on + strand at 399.137 kb on - strand at 399.137 kb on - strand at 399.217 kb on + strand, within ddlA at 399.376 kb on - strand, within ddlA at 399.376 kb on - strand, within ddlA at 399.531 kb on + strand, within ddlA at 399.549 kb on - strand, within ddlA at 399.549 kb on - strand, within ddlA at 399.659 kb on - strand, within ddlA at 399.707 kb on - strand, within ddlA at 399.727 kb on + strand, within ddlA at 399.727 kb on + strand, within ddlA at 399.787 kb on + strand, within ddlA at 399.824 kb on + strand, within ddlA at 399.916 kb on + strand, within ddlA at 399.933 kb on + strand, within ddlA at 399.948 kb on + strand, within ddlA at 399.948 kb on + strand, within ddlA at 400.092 kb on + strand at 400.092 kb on + strand at 400.093 kb on + strand at 400.093 kb on + strand at 400.168 kb on + strand at 400.178 kb on - strand at 400.240 kb on + strand at 400.240 kb on + strand at 400.251 kb on + strand at 400.258 kb on + strand at 400.263 kb on + strand at 400.264 kb on + strand at 400.282 kb on - strand at 400.288 kb on - strand at 400.373 kb on + strand at 400.559 kb on - strand at 400.561 kb on + strand at 400.638 kb on - strand, within yaiB at 400.667 kb on + strand, within yaiB at 400.675 kb on + strand, within yaiB at 400.684 kb on + strand, within yaiB at 400.692 kb on - strand, within yaiB at 400.692 kb on - strand, within yaiB at 400.692 kb on - strand, within yaiB at 400.707 kb on + strand, within yaiB at 400.715 kb on - strand, within yaiB at 400.750 kb on + strand, within yaiB at 400.762 kb on + strand, within yaiB at 400.762 kb on + strand, within yaiB at 400.762 kb on + strand, within yaiB at 400.770 kb on - strand, within yaiB at 400.770 kb on - strand, within yaiB at 400.775 kb on + strand, within yaiB at 400.777 kb on + strand, within yaiB at 400.777 kb on + strand, within yaiB at 400.777 kb on + strand, within yaiB at 400.777 kb on + strand, within yaiB at 400.782 kb on + strand, within yaiB at 400.783 kb on + strand, within yaiB at 400.783 kb on + strand, within yaiB at 400.783 kb on - strand, within yaiB at 400.783 kb on - strand, within yaiB at 400.783 kb on - strand, within yaiB at 400.812 kb on + strand, within yaiB at 400.812 kb on - strand, within yaiB at 400.815 kb on + strand, within yaiB at 400.815 kb on + strand, within yaiB at 400.816 kb on - strand, within yaiB at 400.823 kb on - strand, within yaiB at 400.828 kb on - strand, within yaiB at 400.835 kb on + strand, within yaiB at 400.865 kb on - strand at 400.898 kb on - strand at 400.899 kb on + strand at 400.901 kb on - strand at 400.901 kb on - strand at 400.915 kb on + strand at 400.915 kb on + strand at 400.982 kb on - strand at 400.992 kb on - strand at 400.998 kb on - strand at 401.004 kb on + strand at 401.006 kb on - strand at 401.006 kb on - strand at 401.015 kb on - strand at 401.015 kb on - strand at 401.020 kb on - strand at 401.078 kb on - strand at 401.086 kb on - strand at 401.088 kb on - strand at 401.088 kb on - strand at 401.091 kb on - strand at 401.091 kb on - strand at 401.105 kb on + strand at 401.113 kb on - strand, within phoA at 401.113 kb on - strand, within phoA at 401.113 kb on - strand, within phoA at 401.120 kb on + strand, within phoA at 401.123 kb on + strand, within phoA at 401.123 kb on + strand, within phoA at 401.125 kb on + strand, within phoA at 401.126 kb on - strand, within phoA at 401.133 kb on - strand, within phoA
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source D-Glucose 10 mM remove 398,318 - yaiY b0379 0.22 -0.2 398,351 + yaiY b0379 0.33 -0.1 398,386 - yaiY b0379 0.44 -0.1 398,386 - yaiY b0379 0.44 -0.6 398,392 - yaiY b0379 0.46 +0.1 398,392 - yaiY b0379 0.46 +0.6 398,593 - +0.3 398,608 + +0.5 398,613 - +0.0 398,633 - -0.2 398,755 + +0.3 398,755 + +0.2 398,809 + +0.3 398,852 + b0380 b0380 0.16 +0.0 398,860 - b0380 b0380 0.20 -0.3 398,860 - b0380 b0380 0.20 -0.2 398,883 + b0380 b0380 0.31 +0.3 398,933 + b0380 b0380 0.54 +0.4 398,933 + b0380 b0380 0.54 -0.2 398,933 + b0380 b0380 0.54 -0.1 398,959 - b0380 b0380 0.67 -0.2 398,959 - b0380 b0380 0.67 -1.1 399,034 + +0.2 399,034 + +0.0 399,079 + +0.5 399,092 - -0.1 399,092 - -0.1 399,129 + -0.9 399,129 + +0.6 399,129 + +0.2 399,137 - +0.2 399,137 - +0.6 399,217 + ddlA b0381 0.15 +0.3 399,376 - ddlA b0381 0.29 +0.3 399,376 - ddlA b0381 0.29 +0.7 399,531 + ddlA b0381 0.44 +0.5 399,549 - ddlA b0381 0.45 +0.1 399,549 - ddlA b0381 0.45 +0.7 399,659 - ddlA b0381 0.55 +0.8 399,707 - ddlA b0381 0.60 +0.3 399,727 + ddlA b0381 0.62 -0.0 399,727 + ddlA b0381 0.62 +0.2 399,787 + ddlA b0381 0.67 +0.3 399,824 + ddlA b0381 0.70 +0.0 399,916 + ddlA b0381 0.79 +0.2 399,933 + ddlA b0381 0.80 +0.4 399,948 + ddlA b0381 0.82 +0.8 399,948 + ddlA b0381 0.82 +1.3 400,092 + +0.7 400,092 + +0.7 400,093 + +1.3 400,093 + +1.3 400,168 + +0.7 400,178 - +0.3 400,240 + -0.1 400,240 + -0.4 400,251 + +0.1 400,258 + -0.3 400,263 + +0.7 400,264 + +0.0 400,282 - -0.8 400,288 - -1.0 400,373 + -0.5 400,559 - +0.8 400,561 + -1.6 400,638 - yaiB b0382 0.11 +0.4 400,667 + yaiB b0382 0.22 +0.2 400,675 + yaiB b0382 0.25 -1.0 400,684 + yaiB b0382 0.28 -0.3 400,692 - yaiB b0382 0.31 -0.9 400,692 - yaiB b0382 0.31 +1.5 400,692 - yaiB b0382 0.31 +1.1 400,707 + yaiB b0382 0.37 +1.2 400,715 - yaiB b0382 0.40 -1.0 400,750 + yaiB b0382 0.54 -0.2 400,762 + yaiB b0382 0.58 -0.0 400,762 + yaiB b0382 0.58 +0.5 400,762 + yaiB b0382 0.58 +0.2 400,770 - yaiB b0382 0.61 -0.3 400,770 - yaiB b0382 0.61 +0.3 400,775 + yaiB b0382 0.63 -0.2 400,777 + yaiB b0382 0.64 +0.3 400,777 + yaiB b0382 0.64 -0.2 400,777 + yaiB b0382 0.64 +0.5 400,777 + yaiB b0382 0.64 +0.9 400,782 + yaiB b0382 0.66 -0.0 400,783 + yaiB b0382 0.66 -0.2 400,783 + yaiB b0382 0.66 -0.3 400,783 - yaiB b0382 0.66 -0.3 400,783 - yaiB b0382 0.66 -0.3 400,783 - yaiB b0382 0.66 +0.0 400,812 + yaiB b0382 0.77 +0.0 400,812 - yaiB b0382 0.77 +1.3 400,815 + yaiB b0382 0.79 +0.6 400,815 + yaiB b0382 0.79 +0.3 400,816 - yaiB b0382 0.79 +1.6 400,823 - yaiB b0382 0.82 +0.3 400,828 - yaiB b0382 0.84 +1.8 400,835 + yaiB b0382 0.86 -0.4 400,865 - -0.3 400,898 - +1.3 400,899 + +0.8 400,901 - -0.3 400,901 - +1.0 400,915 + -0.0 400,915 + +0.2 400,982 - -0.1 400,992 - -0.1 400,998 - +0.0 401,004 + +0.7 401,006 - -0.2 401,006 - -1.3 401,015 - +0.5 401,015 - -0.1 401,020 - +0.8 401,078 - +0.1 401,086 - +0.0 401,088 - +0.4 401,088 - +2.3 401,091 - -0.0 401,091 - +0.0 401,105 + -0.3 401,113 - phoA b0383 0.10 +0.5 401,113 - phoA b0383 0.10 -0.7 401,113 - phoA b0383 0.10 +0.1 401,120 + phoA b0383 0.11 -0.3 401,123 + phoA b0383 0.11 +0.4 401,123 + phoA b0383 0.11 +0.1 401,125 + phoA b0383 0.11 +0.4 401,126 - phoA b0383 0.11 +0.1 401,133 - phoA b0383 0.11 +0.4
Or see this region's nucleotide sequence