Strain Fitness in Escherichia coli BW25113 around b0051

Experiment: Carbon source D-Glucose 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntapaH and apaG are separated by 6 nucleotidesapaG and ksgA are separated by 2 nucleotidesksgA and pdxA overlap by 4 nucleotidespdxA and surA overlap by 1 nucleotides b0049: apaH - diadenosinetetraphosphatase (NCBI), at 50,380 to 51,222 apaH b0050: apaG - hypothetical protein (NCBI), at 51,229 to 51,606 apaG b0051: ksgA - dimethyladenosine transferase (NCBI), at 51,609 to 52,430 ksgA b0052: pdxA - 4-hydroxythreonine-4-phosphate dehydrogenase (NCBI), at 52,427 to 53,416 pdxA b0053: surA - peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI), at 53,416 to 54,702 surA Position (kb) 51 52 53Strain fitness (log2 ratio) -2 -1 0 1 2at 50.717 kb on - strand, within apaHat 50.785 kb on - strand, within apaHat 50.791 kb on + strand, within apaHat 50.802 kb on + strand, within apaHat 50.829 kb on - strand, within apaHat 50.859 kb on - strand, within apaHat 50.877 kb on + strand, within apaHat 50.877 kb on + strand, within apaHat 51.025 kb on + strand, within apaHat 51.109 kb on - strand, within apaHat 51.177 kb on + strandat 51.177 kb on + strandat 51.185 kb on - strandat 51.190 kb on + strandat 51.190 kb on + strandat 51.240 kb on + strandat 51.240 kb on + strandat 51.311 kb on + strand, within apaGat 51.311 kb on + strand, within apaGat 51.322 kb on - strand, within apaGat 51.326 kb on - strand, within apaGat 51.334 kb on - strand, within apaGat 51.336 kb on - strand, within apaGat 51.353 kb on + strand, within apaGat 51.353 kb on + strand, within apaGat 51.353 kb on - strand, within apaGat 51.364 kb on - strand, within apaGat 51.372 kb on - strand, within apaGat 51.415 kb on + strand, within apaGat 51.423 kb on - strand, within apaGat 51.466 kb on + strand, within apaGat 51.472 kb on + strand, within apaGat 51.512 kb on - strand, within apaGat 51.514 kb on - strand, within apaGat 51.520 kb on - strand, within apaGat 51.524 kb on + strand, within apaGat 51.550 kb on - strand, within apaGat 51.560 kb on + strand, within apaGat 51.567 kb on - strand, within apaGat 51.567 kb on - strand, within apaGat 51.576 kb on + strandat 51.576 kb on + strandat 51.586 kb on - strandat 51.613 kb on + strandat 51.614 kb on - strandat 51.615 kb on + strandat 51.615 kb on + strandat 51.673 kb on - strandat 51.703 kb on + strand, within ksgAat 51.703 kb on + strand, within ksgAat 51.812 kb on - strand, within ksgAat 51.836 kb on + strand, within ksgAat 51.891 kb on - strand, within ksgAat 51.891 kb on - strand, within ksgAat 51.904 kb on + strand, within ksgAat 51.906 kb on + strand, within ksgAat 51.925 kb on - strand, within ksgAat 52.040 kb on + strand, within ksgAat 52.040 kb on + strand, within ksgAat 52.040 kb on + strand, within ksgAat 52.042 kb on + strand, within ksgAat 52.042 kb on + strand, within ksgAat 52.042 kb on + strand, within ksgAat 52.042 kb on - strand, within ksgAat 52.042 kb on - strand, within ksgAat 52.045 kb on + strand, within ksgAat 52.062 kb on + strand, within ksgAat 52.062 kb on + strandat 52.070 kb on - strand, within ksgAat 52.072 kb on + strand, within ksgAat 52.089 kb on - strand, within ksgAat 52.276 kb on + strand, within ksgAat 52.276 kb on + strand, within ksgAat 52.284 kb on + strand, within ksgAat 52.338 kb on + strand, within ksgAat 52.338 kb on + strand, within ksgAat 52.343 kb on - strand, within ksgAat 52.348 kb on - strandat 52.348 kb on - strandat 52.348 kb on - strandat 52.354 kb on + strandat 52.420 kb on - strandat 52.490 kb on + strandat 52.490 kb on + strandat 52.502 kb on + strandat 52.502 kb on + strandat 52.518 kb on - strandat 52.518 kb on - strandat 52.626 kb on + strand, within pdxAat 52.688 kb on - strand, within pdxAat 52.735 kb on + strand, within pdxAat 52.735 kb on + strand, within pdxAat 52.740 kb on - strand, within pdxAat 52.766 kb on - strand, within pdxAat 52.780 kb on - strand, within pdxAat 52.797 kb on - strand, within pdxAat 52.879 kb on - strand, within pdxAat 52.914 kb on + strand, within pdxAat 52.982 kb on - strand, within pdxAat 52.982 kb on - strand, within pdxAat 52.983 kb on + strand, within pdxAat 53.061 kb on - strand, within pdxAat 53.062 kb on - strand, within pdxAat 53.062 kb on - strandat 53.064 kb on + strand, within pdxAat 53.067 kb on - strand, within pdxAat 53.163 kb on - strand, within pdxAat 53.163 kb on - strand, within pdxAat 53.181 kb on - strand, within pdxAat 53.181 kb on - strand, within pdxAat 53.202 kb on - strand, within pdxAat 53.221 kb on + strand, within pdxAat 53.221 kb on + strand, within pdxAat 53.226 kb on - strand, within pdxAat 53.246 kb on - strand, within pdxAat 53.291 kb on + strand, within pdxAat 53.328 kb on + strandat 53.328 kb on + strandat 53.338 kb on + strandat 53.338 kb on + strandat 53.338 kb on + strandat 53.338 kb on + strandat 53.346 kb on - strandat 53.346 kb on - strandat 53.346 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbon source D-Glucose 10 mM
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50,717 - apaH b0049 0.40 -1.4
50,785 - apaH b0049 0.48 -2.3
50,791 + apaH b0049 0.49 -0.2
50,802 + apaH b0049 0.50 -2.2
50,829 - apaH b0049 0.53 -1.0
50,859 - apaH b0049 0.57 -0.6
50,877 + apaH b0049 0.59 +0.4
50,877 + apaH b0049 0.59 -0.6
51,025 + apaH b0049 0.77 -2.1
51,109 - apaH b0049 0.86 -2.3
51,177 + -1.6
51,177 + -1.8
51,185 - -1.8
51,190 + -1.8
51,190 + -1.8
51,240 + +0.9
51,240 + +0.5
51,311 + apaG b0050 0.22 +0.7
51,311 + apaG b0050 0.22 -0.3
51,322 - apaG b0050 0.25 +0.1
51,326 - apaG b0050 0.26 -0.3
51,334 - apaG b0050 0.28 -0.9
51,336 - apaG b0050 0.28 -0.8
51,353 + apaG b0050 0.33 +0.0
51,353 + apaG b0050 0.33 +0.2
51,353 - apaG b0050 0.33 +0.0
51,364 - apaG b0050 0.36 +0.4
51,372 - apaG b0050 0.38 -1.0
51,415 + apaG b0050 0.49 -0.1
51,423 - apaG b0050 0.51 +0.0
51,466 + apaG b0050 0.63 -2.1
51,472 + apaG b0050 0.64 +0.8
51,512 - apaG b0050 0.75 -0.1
51,514 - apaG b0050 0.75 -0.4
51,520 - apaG b0050 0.77 +0.4
51,524 + apaG b0050 0.78 +0.6
51,550 - apaG b0050 0.85 +0.4
51,560 + apaG b0050 0.88 +0.0
51,567 - apaG b0050 0.89 +0.1
51,567 - apaG b0050 0.89 +0.4
51,576 + -0.1
51,576 + -0.2
51,586 - -0.3
51,613 + -0.2
51,614 - +0.0
51,615 + +0.8
51,615 + -0.1
51,673 - +0.4
51,703 + ksgA b0051 0.11 +0.0
51,703 + ksgA b0051 0.11 -1.4
51,812 - ksgA b0051 0.25 +0.9
51,836 + ksgA b0051 0.28 -0.1
51,891 - ksgA b0051 0.34 +0.0
51,891 - ksgA b0051 0.34 -0.4
51,904 + ksgA b0051 0.36 +1.1
51,906 + ksgA b0051 0.36 +0.8
51,925 - ksgA b0051 0.38 +0.9
52,040 + ksgA b0051 0.52 +0.4
52,040 + ksgA b0051 0.52 +1.4
52,040 + ksgA b0051 0.52 -1.0
52,042 + ksgA b0051 0.53 +1.0
52,042 + ksgA b0051 0.53 +0.4
52,042 + ksgA b0051 0.53 +1.4
52,042 - ksgA b0051 0.53 +1.4
52,042 - ksgA b0051 0.53 -0.1
52,045 + ksgA b0051 0.53 +1.4
52,062 + ksgA b0051 0.55 +0.9
52,062 + +0.0
52,070 - ksgA b0051 0.56 +0.0
52,072 + ksgA b0051 0.56 -1.0
52,089 - ksgA b0051 0.58 -0.6
52,276 + ksgA b0051 0.81 +0.3
52,276 + ksgA b0051 0.81 +0.5
52,284 + ksgA b0051 0.82 +0.1
52,338 + ksgA b0051 0.89 +1.0
52,338 + ksgA b0051 0.89 +1.0
52,343 - ksgA b0051 0.89 +2.1
52,348 - +2.5
52,348 - -0.2
52,348 - -0.3
52,354 + -2.2
52,420 - +0.4
52,490 + -0.0
52,490 + -0.6
52,502 + -0.0
52,502 + +0.8
52,518 - +0.4
52,518 - +0.6
52,626 + pdxA b0052 0.20 -0.6
52,688 - pdxA b0052 0.26 +0.3
52,735 + pdxA b0052 0.31 +0.4
52,735 + pdxA b0052 0.31 -0.9
52,740 - pdxA b0052 0.32 +0.3
52,766 - pdxA b0052 0.34 -0.3
52,780 - pdxA b0052 0.36 -0.7
52,797 - pdxA b0052 0.37 -0.2
52,879 - pdxA b0052 0.46 -0.2
52,914 + pdxA b0052 0.49 -0.3
52,982 - pdxA b0052 0.56 +0.7
52,982 - pdxA b0052 0.56 +0.2
52,983 + pdxA b0052 0.56 -0.2
53,061 - pdxA b0052 0.64 -0.9
53,062 - pdxA b0052 0.64 -0.6
53,062 - +2.4
53,064 + pdxA b0052 0.64 -0.3
53,067 - pdxA b0052 0.65 +1.1
53,163 - pdxA b0052 0.74 +0.5
53,163 - pdxA b0052 0.74 -0.1
53,181 - pdxA b0052 0.76 -0.4
53,181 - pdxA b0052 0.76 -0.1
53,202 - pdxA b0052 0.78 +0.2
53,221 + pdxA b0052 0.80 -0.3
53,221 + pdxA b0052 0.80 +0.1
53,226 - pdxA b0052 0.81 -0.1
53,246 - pdxA b0052 0.83 -0.2
53,291 + pdxA b0052 0.87 -0.1
53,328 + -0.8
53,328 + -0.6
53,338 + -0.0
53,338 + -0.3
53,338 + +0.3
53,338 + -1.1
53,346 - -0.3
53,346 - -0.3
53,346 - +0.0

Or see this region's nucleotide sequence