Strain Fitness in Escherichia coli BW25113 around b2702

Experiment: Carbon source D-Mannitol 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmltB and srlA are separated by 255 nucleotidessrlA and srlE overlap by 4 nucleotidessrlE and srlB are separated by 10 nucleotides b2701: mltB - membrane-bound lytic murein transglycosylase B (NCBI), at 2,822,513 to 2,823,598 mltB b2702: srlA - glucitol/sorbitol-specific enzyme IIC component of PTS (NCBI), at 2,823,854 to 2,824,417 srlA b2703: srlE - glucitol/sorbitol-specific enzyme IIB component of PTS (NCBI), at 2,824,414 to 2,825,373 srlE b2704: srlB - glucitol/sorbitol-specific enzyme IIA component of PTS (NCBI), at 2,825,384 to 2,825,755 srlB Position (kb) 2823 2824 2825Strain fitness (log2 ratio) -2 -1 0 1at 2822.880 kb on - strand, within mltBat 2822.996 kb on + strand, within mltBat 2823.026 kb on - strand, within mltBat 2823.150 kb on + strand, within mltBat 2823.162 kb on + strand, within mltBat 2823.183 kb on + strand, within mltBat 2823.256 kb on + strand, within mltBat 2823.279 kb on - strand, within mltBat 2823.486 kb on - strand, within mltBat 2823.486 kb on - strand, within mltBat 2823.519 kb on - strandat 2823.519 kb on - strandat 2823.633 kb on + strandat 2823.673 kb on - strandat 2823.673 kb on - strandat 2823.689 kb on - strandat 2823.807 kb on - strandat 2823.849 kb on + strandat 2823.872 kb on - strandat 2823.876 kb on + strandat 2823.939 kb on + strand, within srlAat 2823.939 kb on + strand, within srlAat 2823.970 kb on - strand, within srlAat 2823.976 kb on - strand, within srlAat 2824.104 kb on + strand, within srlAat 2824.104 kb on + strand, within srlAat 2824.117 kb on - strand, within srlAat 2824.196 kb on + strand, within srlAat 2824.229 kb on - strand, within srlAat 2824.229 kb on - strand, within srlAat 2824.287 kb on + strand, within srlAat 2824.287 kb on + strand, within srlAat 2824.300 kb on - strand, within srlAat 2824.412 kb on - strandat 2824.412 kb on - strandat 2824.460 kb on + strandat 2824.683 kb on - strand, within srlEat 2824.736 kb on - strand, within srlEat 2824.775 kb on + strand, within srlEat 2824.933 kb on - strand, within srlEat 2824.938 kb on - strand, within srlEat 2824.938 kb on - strand, within srlEat 2824.969 kb on + strand, within srlEat 2824.969 kb on + strand, within srlEat 2824.983 kb on + strand, within srlEat 2824.983 kb on + strand, within srlEat 2825.009 kb on + strand, within srlEat 2825.113 kb on - strand, within srlEat 2825.179 kb on + strand, within srlEat 2825.263 kb on + strand, within srlEat 2825.263 kb on + strand, within srlEat 2825.296 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbon source D-Mannitol 5 mM
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2,822,880 - mltB b2701 0.34 +0.2
2,822,996 + mltB b2701 0.44 -0.2
2,823,026 - mltB b2701 0.47 +0.6
2,823,150 + mltB b2701 0.59 -0.6
2,823,162 + mltB b2701 0.60 -0.5
2,823,183 + mltB b2701 0.62 -0.2
2,823,256 + mltB b2701 0.68 +0.2
2,823,279 - mltB b2701 0.71 +0.4
2,823,486 - mltB b2701 0.90 -0.4
2,823,486 - mltB b2701 0.90 +0.7
2,823,519 - +0.1
2,823,519 - +0.7
2,823,633 + +1.2
2,823,673 - +0.1
2,823,673 - -0.2
2,823,689 - -0.5
2,823,807 - +0.6
2,823,849 + +0.5
2,823,872 - +0.5
2,823,876 + -0.4
2,823,939 + srlA b2702 0.15 +0.5
2,823,939 + srlA b2702 0.15 +0.8
2,823,970 - srlA b2702 0.21 +0.2
2,823,976 - srlA b2702 0.22 +0.0
2,824,104 + srlA b2702 0.44 +1.2
2,824,104 + srlA b2702 0.44 +1.3
2,824,117 - srlA b2702 0.47 -0.2
2,824,196 + srlA b2702 0.61 +0.0
2,824,229 - srlA b2702 0.66 +0.6
2,824,229 - srlA b2702 0.66 +0.5
2,824,287 + srlA b2702 0.77 +1.4
2,824,287 + srlA b2702 0.77 +0.3
2,824,300 - srlA b2702 0.79 -0.2
2,824,412 - -0.1
2,824,412 - -2.5
2,824,460 + +0.2
2,824,683 - srlE b2703 0.28 +0.6
2,824,736 - srlE b2703 0.34 +0.3
2,824,775 + srlE b2703 0.38 +0.6
2,824,933 - srlE b2703 0.54 +0.7
2,824,938 - srlE b2703 0.55 -0.7
2,824,938 - srlE b2703 0.55 -0.6
2,824,969 + srlE b2703 0.58 +0.1
2,824,969 + srlE b2703 0.58 +0.1
2,824,983 + srlE b2703 0.59 +0.5
2,824,983 + srlE b2703 0.59 -1.7
2,825,009 + srlE b2703 0.62 -0.1
2,825,113 - srlE b2703 0.73 -0.1
2,825,179 + srlE b2703 0.80 -0.4
2,825,263 + srlE b2703 0.88 +0.6
2,825,263 + srlE b2703 0.88 +0.3
2,825,296 + -0.0

Or see this region's nucleotide sequence