Experiment: Carbon source D-Mannitol 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mazG and chpA are separated by 70 nucleotides chpA and chpR overlap by 1 nucleotides chpR and relA are separated by 77 nucleotides
b2781: mazG - nucleoside triphosphate pyrophosphohydrolase (NCBI), at 2,907,916 to 2,908,707
mazG
b2782: chpA - toxin of the ChpA-ChpR toxin-antitoxin system, endoribonuclease (NCBI), at 2,908,778 to 2,909,113
chpA
b2783: chpR - antitoxin of the ChpA-ChpR toxin-antitoxin system (NCBI), at 2,909,113 to 2,909,361
chpR
b2784: relA - (p)ppGpp synthetase I/GTP pyrophosphokinase (NCBI), at 2,909,439 to 2,911,673
relA
Position (kb)
2909
2910 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2908.122 kb on - strand, within mazG at 2908.157 kb on - strand, within mazG at 2908.172 kb on + strand, within mazG at 2908.172 kb on + strand, within mazG at 2908.377 kb on - strand, within mazG at 2908.538 kb on + strand, within mazG at 2908.571 kb on + strand, within mazG at 2908.624 kb on + strand, within mazG at 2908.674 kb on + strand at 2908.674 kb on + strand at 2908.674 kb on + strand at 2908.674 kb on + strand at 2908.682 kb on - strand at 2908.754 kb on - strand at 2908.754 kb on - strand at 2908.764 kb on - strand at 2908.764 kb on - strand at 2908.772 kb on + strand at 2908.772 kb on + strand at 2908.854 kb on + strand, within chpA at 2908.991 kb on - strand, within chpA at 2909.017 kb on - strand, within chpA at 2909.017 kb on - strand, within chpA at 2909.032 kb on + strand, within chpA at 2909.032 kb on + strand, within chpA at 2909.253 kb on + strand, within chpR at 2909.258 kb on + strand, within chpR at 2909.311 kb on + strand, within chpR at 2909.487 kb on + strand at 2909.487 kb on + strand at 2909.490 kb on - strand at 2909.490 kb on - strand at 2909.556 kb on - strand at 2909.669 kb on - strand, within relA at 2909.776 kb on + strand, within relA at 2909.776 kb on + strand, within relA at 2910.051 kb on - strand, within relA at 2910.051 kb on - strand, within relA at 2910.174 kb on + strand, within relA at 2910.174 kb on + strand, within relA at 2910.294 kb on - strand, within relA at 2910.313 kb on + strand, within relA
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source D-Mannitol 5 mM remove 2,908,122 - mazG b2781 0.26 +0.0 2,908,157 - mazG b2781 0.30 +0.1 2,908,172 + mazG b2781 0.32 +0.8 2,908,172 + mazG b2781 0.32 -0.6 2,908,377 - mazG b2781 0.58 -0.0 2,908,538 + mazG b2781 0.79 -0.7 2,908,571 + mazG b2781 0.83 -0.2 2,908,624 + mazG b2781 0.89 -0.0 2,908,674 + +0.1 2,908,674 + +0.3 2,908,674 + +0.9 2,908,674 + +0.1 2,908,682 - +0.0 2,908,754 - +0.1 2,908,754 - -0.2 2,908,764 - -0.9 2,908,764 - -0.8 2,908,772 + +0.4 2,908,772 + +0.0 2,908,854 + chpA b2782 0.23 -0.4 2,908,991 - chpA b2782 0.63 +0.4 2,909,017 - chpA b2782 0.71 +0.4 2,909,017 - chpA b2782 0.71 +0.7 2,909,032 + chpA b2782 0.76 +0.1 2,909,032 + chpA b2782 0.76 +0.0 2,909,253 + chpR b2783 0.56 +0.9 2,909,258 + chpR b2783 0.58 +0.4 2,909,311 + chpR b2783 0.80 +0.5 2,909,487 + -3.3 2,909,487 + -3.1 2,909,490 - -2.5 2,909,490 - -3.0 2,909,556 - -1.7 2,909,669 - relA b2784 0.10 -4.1 2,909,776 + relA b2784 0.15 -3.4 2,909,776 + relA b2784 0.15 -2.8 2,910,051 - relA b2784 0.27 -2.4 2,910,051 - relA b2784 0.27 -5.3 2,910,174 + relA b2784 0.33 -3.5 2,910,174 + relA b2784 0.33 -1.8 2,910,294 - relA b2784 0.38 -5.8 2,910,313 + relA b2784 0.39 -3.7
Or see this region's nucleotide sequence