Experiment: Carbon source D-Mannitol 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ynhE and sufA are separated by 8 nucleotides sufA and rydB are separated by 326 nucleotides rydB and ydiH are separated by 153 nucleotides ydiH and ydiI are separated by 99 nucleotides ydiI and ydiJ overlap by 4 nucleotides
b1683: ynhE - orf, hypothetical protein (VIMSS), at 1,760,546 to 1,762,033
ynhE
b1684: sufA - iron-sulfur cluster assembly scaffold protein (NCBI), at 1,762,042 to 1,762,410
sufA
b4430: rydB - regulatory sRNA (NCBI), at 1,762,737 to 1,762,804
rydB
b1685: ydiH - predicted protein (RefSeq), at 1,762,958 to 1,763,146
ydiH
b1686: ydiI - hypothetical protein (NCBI), at 1,763,246 to 1,763,656
ydiI
b1687: ydiJ - predicted FAD-linked oxidoreductase (NCBI), at 1,763,653 to 1,766,709
ydiJ
Position (kb)
1762
1763
1764 Strain fitness (log2 ratio)
-1
0
1 at 1762.235 kb on - strand, within sufA at 1762.235 kb on - strand, within sufA at 1762.245 kb on - strand, within sufA at 1762.437 kb on + strand at 1762.521 kb on + strand at 1762.546 kb on - strand at 1762.891 kb on - strand at 1762.891 kb on - strand at 1762.904 kb on - strand at 1763.084 kb on - strand, within ydiH at 1763.150 kb on - strand at 1763.150 kb on - strand at 1763.186 kb on - strand at 1763.195 kb on + strand at 1763.195 kb on + strand at 1763.545 kb on + strand, within ydiI at 1763.577 kb on - strand, within ydiI at 1763.577 kb on - strand, within ydiI at 1763.692 kb on - strand at 1763.886 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source D-Mannitol 5 mM remove 1,762,235 - sufA b1684 0.52 -0.3 1,762,235 - sufA b1684 0.52 -0.5 1,762,245 - sufA b1684 0.55 -0.5 1,762,437 + +0.3 1,762,521 + +0.2 1,762,546 - +0.2 1,762,891 - +0.4 1,762,891 - +0.8 1,762,904 - -0.2 1,763,084 - ydiH b1685 0.67 +0.4 1,763,150 - +0.4 1,763,150 - +0.6 1,763,186 - -0.8 1,763,195 + +0.1 1,763,195 + +0.7 1,763,545 + ydiI b1686 0.73 +0.8 1,763,577 - ydiI b1686 0.81 -0.3 1,763,577 - ydiI b1686 0.81 -0.5 1,763,692 - -1.0 1,763,886 - -0.3
Or see this region's nucleotide sequence