Strain Fitness in Escherichia coli BW25113 around b1650

Experiment: Carbon source D-Mannitol 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntb1648 and ydhM are separated by 102 nucleotidesydhM and nemA are separated by 36 nucleotidesnemA and gloA are separated by 80 nucleotidesgloA and rnt are separated by 102 nucleotides b1648: b1648 - orf, hypothetical protein (VIMSS), at 1,723,705 to 1,723,944 b1648 b1649: ydhM - predicted DNA-binding transcriptional regulator (RefSeq), at 1,724,047 to 1,724,646 ydhM b1650: nemA - N-ethylmaleimide reductase, FMN-linked (NCBI), at 1,724,683 to 1,725,780 nemA b1651: gloA - glyoxalase I, Ni-dependent (NCBI), at 1,725,861 to 1,726,268 gloA b1652: rnt - ribonuclease T (NCBI), at 1,726,371 to 1,727,018 rnt Position (kb) 1724 1725 1726Strain fitness (log2 ratio) -1 0 1at 1723.797 kb on - strand, within b1648at 1724.028 kb on + strandat 1724.772 kb on - strandat 1725.072 kb on + strand, within nemAat 1725.075 kb on - strand, within nemAat 1725.075 kb on - strand, within nemAat 1725.118 kb on - strand, within nemAat 1725.233 kb on - strand, within nemAat 1725.233 kb on - strand, within nemAat 1725.403 kb on + strand, within nemAat 1725.437 kb on + strand, within nemAat 1725.754 kb on + strandat 1725.761 kb on - strandat 1725.763 kb on - strandat 1725.763 kb on - strandat 1725.877 kb on - strandat 1725.877 kb on - strandat 1726.040 kb on - strand, within gloAat 1726.072 kb on - strand, within gloAat 1726.092 kb on + strand, within gloAat 1726.185 kb on - strand, within gloAat 1726.185 kb on - strand, within gloA

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbon source D-Mannitol 5 mM
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1,723,797 - b1648 b1648 0.38 +0.0
1,724,028 + +0.5
1,724,772 - +0.4
1,725,072 + nemA b1650 0.35 +1.2
1,725,075 - nemA b1650 0.36 +0.3
1,725,075 - nemA b1650 0.36 +0.5
1,725,118 - nemA b1650 0.40 -0.0
1,725,233 - nemA b1650 0.50 -0.6
1,725,233 - nemA b1650 0.50 +0.6
1,725,403 + nemA b1650 0.66 +0.4
1,725,437 + nemA b1650 0.69 +0.4
1,725,754 + -0.4
1,725,761 - -0.8
1,725,763 - +0.4
1,725,763 - -0.5
1,725,877 - +0.5
1,725,877 - -0.6
1,726,040 - gloA b1651 0.44 +0.2
1,726,072 - gloA b1651 0.52 -0.3
1,726,092 + gloA b1651 0.57 +0.4
1,726,185 - gloA b1651 0.79 +0.7
1,726,185 - gloA b1651 0.79 -0.5

Or see this region's nucleotide sequence