Strain Fitness in Escherichia coli BW25113 around b1335

Experiment: Carbon source D-Mannitol 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfnr and ogt are separated by 194 nucleotidesogt and ydaH are separated by 10 nucleotides b1334: fnr - DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI), at 1,396,798 to 1,397,550 fnr b1335: ogt - O-6-alkylguanine-DNA:cysteine-protein methyltransferase (NCBI), at 1,397,745 to 1,398,260 ogt b1336: ydaH - putative pump protein (transport) (VIMSS), at 1,398,271 to 1,399,797 ydaH Position (kb) 1397 1398 1399Strain fitness (log2 ratio) -1 0 1at 1397.086 kb on + strand, within fnrat 1397.086 kb on + strand, within fnrat 1397.125 kb on - strand, within fnrat 1397.125 kb on - strand, within fnrat 1397.287 kb on - strand, within fnrat 1397.605 kb on + strandat 1397.605 kb on + strandat 1397.857 kb on - strand, within ogtat 1397.857 kb on - strand, within ogtat 1397.903 kb on + strand, within ogtat 1397.903 kb on + strand, within ogtat 1398.130 kb on + strand, within ogtat 1398.130 kb on + strand, within ogtat 1398.138 kb on + strand, within ogtat 1398.138 kb on + strand, within ogtat 1398.666 kb on + strand, within ydaHat 1398.666 kb on + strand, within ydaHat 1398.781 kb on - strand, within ydaHat 1398.822 kb on - strand, within ydaHat 1398.822 kb on - strand, within ydaHat 1398.846 kb on - strand, within ydaHat 1398.968 kb on - strand, within ydaHat 1398.968 kb on - strand, within ydaH

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbon source D-Mannitol 5 mM
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1,397,086 + fnr b1334 0.38 -0.5
1,397,086 + fnr b1334 0.38 -0.7
1,397,125 - fnr b1334 0.43 +1.0
1,397,125 - fnr b1334 0.43 +1.4
1,397,287 - fnr b1334 0.65 +0.0
1,397,605 + -0.1
1,397,605 + +0.3
1,397,857 - ogt b1335 0.22 +0.4
1,397,857 - ogt b1335 0.22 +1.2
1,397,903 + ogt b1335 0.31 -0.7
1,397,903 + ogt b1335 0.31 +0.7
1,398,130 + ogt b1335 0.75 +1.0
1,398,130 + ogt b1335 0.75 -0.1
1,398,138 + ogt b1335 0.76 +0.1
1,398,138 + ogt b1335 0.76 +0.2
1,398,666 + ydaH b1336 0.26 +0.7
1,398,666 + ydaH b1336 0.26 +0.3
1,398,781 - ydaH b1336 0.33 -0.3
1,398,822 - ydaH b1336 0.36 -0.1
1,398,822 - ydaH b1336 0.36 -0.1
1,398,846 - ydaH b1336 0.38 +0.2
1,398,968 - ydaH b1336 0.46 +0.1
1,398,968 - ydaH b1336 0.46 +0.1

Or see this region's nucleotide sequence