Strain Fitness in Escherichia coli BW25113 around b1122

Experiment: Carbon source D-Mannitol 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcobB and ycfZ are separated by 119 nucleotidesycfZ and ymfA overlap by 4 nucleotidesymfA and potD are separated by 57 nucleotides b1120: cobB - deacetylase of acs and cheY, regulates chemotaxis (NCBI), at 1,178,854 to 1,179,582 cobB b1121: ycfZ - predicted inner membrane protein (NCBI), at 1,179,702 to 1,180,490 ycfZ b1122: ymfA - orf, hypothetical protein (VIMSS), at 1,180,487 to 1,180,948 ymfA b1123: potD - spermidine/putrescine ABC transporter periplasmic substrate-binding protein (NCBI), at 1,181,006 to 1,182,052 potD Position (kb) 1180 1181Strain fitness (log2 ratio) -1 0 1 2at 1179.526 kb on + strandat 1179.552 kb on - strandat 1179.584 kb on + strandat 1179.584 kb on + strandat 1179.584 kb on + strandat 1179.594 kb on + strandat 1179.758 kb on - strandat 1179.768 kb on - strandat 1179.785 kb on + strand, within ycfZat 1179.833 kb on + strand, within ycfZat 1179.837 kb on - strand, within ycfZat 1179.922 kb on + strand, within ycfZat 1179.922 kb on + strand, within ycfZat 1179.922 kb on + strand, within ycfZat 1179.922 kb on + strand, within ycfZat 1179.922 kb on + strand, within ycfZat 1179.943 kb on + strand, within ycfZat 1179.992 kb on + strand, within ycfZat 1180.234 kb on - strand, within ycfZat 1180.389 kb on - strand, within ycfZat 1180.389 kb on - strand, within ycfZat 1180.438 kb on + strandat 1180.457 kb on + strandat 1180.739 kb on - strand, within ymfAat 1180.755 kb on + strand, within ymfAat 1180.755 kb on - strand, within ymfAat 1180.755 kb on - strand, within ymfAat 1180.763 kb on - strand, within ymfAat 1180.777 kb on - strand, within ymfAat 1180.777 kb on - strand, within ymfAat 1180.777 kb on - strand, within ymfAat 1180.777 kb on - strand, within ymfAat 1180.777 kb on - strand, within ymfAat 1180.777 kb on - strand, within ymfAat 1180.778 kb on - strand, within ymfAat 1180.796 kb on + strand, within ymfAat 1180.796 kb on + strand, within ymfAat 1180.799 kb on - strand, within ymfAat 1180.800 kb on - strand, within ymfAat 1180.803 kb on - strand, within ymfAat 1180.809 kb on + strand, within ymfAat 1180.813 kb on + strand, within ymfAat 1180.817 kb on - strand, within ymfAat 1180.834 kb on - strand, within ymfAat 1180.838 kb on - strand, within ymfAat 1180.854 kb on - strand, within ymfAat 1180.869 kb on - strand, within ymfAat 1180.881 kb on + strand, within ymfAat 1180.881 kb on + strand, within ymfAat 1180.890 kb on + strand, within ymfAat 1180.894 kb on + strand, within ymfAat 1180.894 kb on + strand, within ymfAat 1180.894 kb on + strand, within ymfAat 1180.900 kb on + strand, within ymfAat 1180.900 kb on + strand, within ymfAat 1180.902 kb on - strandat 1180.908 kb on - strandat 1180.937 kb on + strandat 1180.945 kb on - strandat 1180.945 kb on - strandat 1180.953 kb on - strandat 1180.959 kb on - strandat 1180.988 kb on - strandat 1180.988 kb on - strandat 1180.989 kb on - strandat 1180.989 kb on - strandat 1181.036 kb on - strandat 1181.046 kb on - strandat 1181.101 kb on + strandat 1181.101 kb on + strandat 1181.170 kb on - strand, within potDat 1181.280 kb on - strand, within potDat 1181.280 kb on - strand, within potDat 1181.421 kb on + strand, within potDat 1181.480 kb on - strand, within potDat 1181.522 kb on - strand, within potDat 1181.539 kb on + strand, within potDat 1181.547 kb on - strand, within potDat 1181.547 kb on - strand, within potDat 1181.556 kb on + strand, within potDat 1181.556 kb on + strand, within potDat 1181.581 kb on - strand, within potDat 1181.684 kb on - strand, within potDat 1181.721 kb on - strand, within potDat 1181.839 kb on - strand, within potDat 1181.865 kb on - strand, within potDat 1181.918 kb on + strand, within potD

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbon source D-Mannitol 5 mM
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1,179,526 + -1.3
1,179,552 - +1.3
1,179,584 + -0.7
1,179,584 + +0.1
1,179,584 + +0.1
1,179,594 + +0.3
1,179,758 - -0.3
1,179,768 - +2.3
1,179,785 + ycfZ b1121 0.11 +0.8
1,179,833 + ycfZ b1121 0.17 +0.0
1,179,837 - ycfZ b1121 0.17 +0.3
1,179,922 + ycfZ b1121 0.28 +0.3
1,179,922 + ycfZ b1121 0.28 +0.3
1,179,922 + ycfZ b1121 0.28 +1.3
1,179,922 + ycfZ b1121 0.28 -0.7
1,179,922 + ycfZ b1121 0.28 +2.3
1,179,943 + ycfZ b1121 0.31 +1.3
1,179,992 + ycfZ b1121 0.37 +1.3
1,180,234 - ycfZ b1121 0.67 +1.3
1,180,389 - ycfZ b1121 0.87 +0.3
1,180,389 - ycfZ b1121 0.87 -0.3
1,180,438 + +1.3
1,180,457 + +1.3
1,180,739 - ymfA b1122 0.55 +0.9
1,180,755 + ymfA b1122 0.58 +1.3
1,180,755 - ymfA b1122 0.58 -1.5
1,180,755 - ymfA b1122 0.58 +0.8
1,180,763 - ymfA b1122 0.60 -0.7
1,180,777 - ymfA b1122 0.63 +0.7
1,180,777 - ymfA b1122 0.63 +1.4
1,180,777 - ymfA b1122 0.63 -0.4
1,180,777 - ymfA b1122 0.63 +0.3
1,180,777 - ymfA b1122 0.63 +0.3
1,180,777 - ymfA b1122 0.63 +0.2
1,180,778 - ymfA b1122 0.63 +1.1
1,180,796 + ymfA b1122 0.67 -0.7
1,180,796 + ymfA b1122 0.67 +0.3
1,180,799 - ymfA b1122 0.68 +0.5
1,180,800 - ymfA b1122 0.68 +1.3
1,180,803 - ymfA b1122 0.68 +0.1
1,180,809 + ymfA b1122 0.70 +0.7
1,180,813 + ymfA b1122 0.71 +0.5
1,180,817 - ymfA b1122 0.71 +0.7
1,180,834 - ymfA b1122 0.75 +0.9
1,180,838 - ymfA b1122 0.76 +0.7
1,180,854 - ymfA b1122 0.79 +2.6
1,180,869 - ymfA b1122 0.83 +0.4
1,180,881 + ymfA b1122 0.85 +0.0
1,180,881 + ymfA b1122 0.85 -1.7
1,180,890 + ymfA b1122 0.87 -0.0
1,180,894 + ymfA b1122 0.88 +0.4
1,180,894 + ymfA b1122 0.88 +0.5
1,180,894 + ymfA b1122 0.88 +0.7
1,180,900 + ymfA b1122 0.89 +0.0
1,180,900 + ymfA b1122 0.89 +0.5
1,180,902 - +0.1
1,180,908 - -0.3
1,180,937 + -0.2
1,180,945 - +1.6
1,180,945 - +0.8
1,180,953 - -0.4
1,180,959 - -0.2
1,180,988 - +0.8
1,180,988 - +0.8
1,180,989 - +0.4
1,180,989 - +1.6
1,181,036 - -0.9
1,181,046 - +0.2
1,181,101 + -1.7
1,181,101 + -1.7
1,181,170 - potD b1123 0.16 +0.8
1,181,280 - potD b1123 0.26 +0.8
1,181,280 - potD b1123 0.26 +0.3
1,181,421 + potD b1123 0.40 +0.2
1,181,480 - potD b1123 0.45 -0.4
1,181,522 - potD b1123 0.49 +0.3
1,181,539 + potD b1123 0.51 -0.1
1,181,547 - potD b1123 0.52 +0.3
1,181,547 - potD b1123 0.52 +0.8
1,181,556 + potD b1123 0.53 +1.6
1,181,556 + potD b1123 0.53 -1.6
1,181,581 - potD b1123 0.55 +0.1
1,181,684 - potD b1123 0.65 -0.1
1,181,721 - potD b1123 0.68 +1.7
1,181,839 - potD b1123 0.80 +0.6
1,181,865 - potD b1123 0.82 -1.1
1,181,918 + potD b1123 0.87 +1.1

Or see this region's nucleotide sequence