Experiment: Carbon source D-Mannitol 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt insB-4 and cspH are separated by 432 nucleotides cspH and cspG are separated by 285 nucleotides cspG and ymcE are separated by 173 nucleotides ymcE and gnsA overlap by 11 nucleotides gnsA and yccM are separated by 48 nucleotides
b0988: insB-4 - IS1 transposase InsAB' (NCBI), at 1,049,250 to 1,049,753
insB-4
b0989: cspH - stress protein, member of the CspA-family (NCBI), at 1,050,186 to 1,050,398
cspH
b0990: cspG - DNA-binding transcriptional regulator (NCBI), at 1,050,684 to 1,050,896
cspG
b0991: ymcE - cold shock gene (NCBI), at 1,051,070 to 1,051,300
ymcE
b4517: gnsA - predicted regulator of phosphatidylethanolamine synthesis (NCBI), at 1,051,290 to 1,051,463
gnsA
b0992: yccM - predicted 4Fe-4S membrane protein (NCBI), at 1,051,512 to 1,052,585
yccM
Position (kb)
1050
1051 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1049.781 kb on - strand at 1049.781 kb on - strand at 1049.782 kb on - strand at 1049.788 kb on - strand at 1049.795 kb on - strand at 1049.795 kb on - strand at 1049.809 kb on - strand at 1049.835 kb on + strand at 1049.835 kb on + strand at 1049.843 kb on - strand at 1049.852 kb on - strand at 1049.854 kb on + strand at 1049.854 kb on + strand at 1049.854 kb on + strand at 1049.856 kb on + strand at 1049.856 kb on + strand at 1049.857 kb on - strand at 1049.862 kb on - strand at 1049.862 kb on - strand at 1049.862 kb on - strand at 1049.918 kb on - strand at 1049.920 kb on + strand at 1049.940 kb on - strand at 1049.967 kb on - strand at 1050.022 kb on + strand at 1050.031 kb on - strand at 1050.033 kb on - strand at 1050.045 kb on - strand at 1050.049 kb on + strand at 1050.062 kb on + strand at 1050.149 kb on + strand at 1050.154 kb on + strand at 1050.164 kb on + strand at 1050.180 kb on + strand at 1050.180 kb on + strand at 1050.184 kb on + strand at 1050.184 kb on + strand at 1050.184 kb on + strand at 1050.184 kb on + strand at 1050.194 kb on + strand at 1050.194 kb on + strand at 1050.211 kb on + strand, within cspH at 1050.211 kb on + strand, within cspH at 1050.226 kb on + strand, within cspH at 1050.254 kb on + strand, within cspH at 1050.254 kb on + strand, within cspH at 1050.269 kb on + strand, within cspH at 1050.269 kb on + strand, within cspH at 1050.277 kb on - strand, within cspH at 1050.277 kb on - strand, within cspH at 1050.336 kb on + strand, within cspH at 1050.349 kb on + strand, within cspH at 1050.398 kb on + strand at 1050.398 kb on + strand at 1050.435 kb on - strand at 1050.435 kb on - strand at 1050.516 kb on + strand at 1050.565 kb on - strand at 1050.590 kb on + strand at 1050.613 kb on + strand at 1050.613 kb on + strand at 1050.733 kb on + strand, within cspG at 1050.756 kb on - strand, within cspG at 1050.756 kb on - strand, within cspG at 1050.883 kb on + strand at 1050.944 kb on + strand at 1050.944 kb on + strand at 1050.949 kb on + strand at 1050.949 kb on + strand at 1050.949 kb on + strand at 1050.955 kb on - strand at 1050.957 kb on - strand at 1050.970 kb on - strand at 1051.100 kb on - strand, within ymcE at 1051.100 kb on - strand, within ymcE at 1051.107 kb on + strand, within ymcE at 1051.107 kb on + strand, within ymcE at 1051.149 kb on + strand, within ymcE at 1051.149 kb on + strand, within ymcE at 1051.151 kb on + strand, within ymcE at 1051.161 kb on - strand, within ymcE at 1051.161 kb on - strand, within ymcE at 1051.203 kb on + strand, within ymcE at 1051.203 kb on + strand, within ymcE at 1051.228 kb on + strand, within ymcE at 1051.255 kb on - strand, within ymcE at 1051.480 kb on - strand at 1051.591 kb on - strand at 1051.631 kb on - strand, within yccM at 1051.640 kb on + strand, within yccM at 1051.714 kb on - strand, within yccM at 1051.717 kb on + strand, within yccM at 1051.735 kb on - strand, within yccM at 1051.770 kb on - strand, within yccM at 1051.872 kb on - strand, within yccM
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source D-Mannitol 5 mM remove 1,049,781 - +0.2 1,049,781 - +0.2 1,049,782 - +1.1 1,049,788 - -0.1 1,049,795 - -0.1 1,049,795 - -0.1 1,049,809 - -0.4 1,049,835 + +0.1 1,049,835 + -0.5 1,049,843 - -0.8 1,049,852 - -0.1 1,049,854 + -0.0 1,049,854 + +0.3 1,049,854 + +0.3 1,049,856 + -0.4 1,049,856 + +1.2 1,049,857 - +0.1 1,049,862 - -0.3 1,049,862 - -0.3 1,049,862 - +1.1 1,049,918 - +0.6 1,049,920 + -2.2 1,049,940 - +0.7 1,049,967 - +0.2 1,050,022 + -0.3 1,050,031 - -2.9 1,050,033 - +0.9 1,050,045 - -0.4 1,050,049 + +0.2 1,050,062 + -0.6 1,050,149 + +0.4 1,050,154 + +0.0 1,050,164 + +0.5 1,050,180 + -0.4 1,050,180 + -0.8 1,050,184 + +0.5 1,050,184 + -0.3 1,050,184 + +0.6 1,050,184 + +0.4 1,050,194 + -0.3 1,050,194 + -0.2 1,050,211 + cspH b0989 0.12 +0.7 1,050,211 + cspH b0989 0.12 +0.2 1,050,226 + cspH b0989 0.19 +1.7 1,050,254 + cspH b0989 0.32 +0.9 1,050,254 + cspH b0989 0.32 +1.3 1,050,269 + cspH b0989 0.39 -1.7 1,050,269 + cspH b0989 0.39 +0.5 1,050,277 - cspH b0989 0.43 +0.3 1,050,277 - cspH b0989 0.43 +1.3 1,050,336 + cspH b0989 0.70 +0.8 1,050,349 + cspH b0989 0.77 +1.3 1,050,398 + +1.3 1,050,398 + +0.3 1,050,435 - +1.3 1,050,435 - +1.3 1,050,516 + +1.3 1,050,565 - +0.3 1,050,590 + -0.1 1,050,613 + +0.0 1,050,613 + +0.5 1,050,733 + cspG b0990 0.23 +0.3 1,050,756 - cspG b0990 0.34 +1.3 1,050,756 - cspG b0990 0.34 +1.0 1,050,883 + -0.1 1,050,944 + -0.4 1,050,944 + +0.8 1,050,949 + +0.2 1,050,949 + +0.4 1,050,949 + -0.1 1,050,955 - +0.5 1,050,957 - -0.2 1,050,970 - +0.1 1,051,100 - ymcE b0991 0.13 +0.3 1,051,100 - ymcE b0991 0.13 -2.3 1,051,107 + ymcE b0991 0.16 -1.7 1,051,107 + ymcE b0991 0.16 +0.3 1,051,149 + ymcE b0991 0.34 +0.0 1,051,149 + ymcE b0991 0.34 -1.5 1,051,151 + ymcE b0991 0.35 +0.1 1,051,161 - ymcE b0991 0.39 +0.2 1,051,161 - ymcE b0991 0.39 +0.1 1,051,203 + ymcE b0991 0.58 +0.1 1,051,203 + ymcE b0991 0.58 +0.0 1,051,228 + ymcE b0991 0.68 -0.7 1,051,255 - ymcE b0991 0.80 +0.1 1,051,480 - +0.5 1,051,591 - +0.1 1,051,631 - yccM b0992 0.11 +0.1 1,051,640 + yccM b0992 0.12 +1.3 1,051,714 - yccM b0992 0.19 +0.4 1,051,717 + yccM b0992 0.19 -0.2 1,051,735 - yccM b0992 0.21 -0.0 1,051,770 - yccM b0992 0.24 -0.3 1,051,872 - yccM b0992 0.34 +0.3
Or see this region's nucleotide sequence