Strain Fitness in Pseudomonas putida KT2440 around PP_3305

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
3,739,981	+		PP_3304	0.34	+0.4
3,739,982	-		PP_3304	0.34	-0.8
3,740,106	-		PP_3304	0.44	+0.6
3,740,106	-		PP_3304	0.44	-0.1
3,740,157	-		PP_3304	0.48	-0.3
3,740,157	-		PP_3304	0.48	+0.0
3,740,288	+		PP_3304	0.59	-0.0
3,740,288	+		PP_3304	0.59	+0.4
3,740,289	-		PP_3304	0.59	+0.0
3,740,363	-		PP_3304	0.65	+1.1
3,740,363	-		PP_3304	0.65	-0.3
3,740,472	+		PP_3304	0.74	+0.1
3,740,525	-		PP_3304	0.78	-0.3
3,740,550	-		PP_3304	0.80	-0.1
3,740,562	+		PP_3304	0.81	+0.1
3,740,563	-		PP_3304	0.81	+0.8
3,740,767	+				+0.5
3,740,767	+				-0.3
3,740,768	-				-0.3
3,740,779	-				+0.3
3,740,800	+				-0.0
3,740,801	-				-0.0
3,740,801	-				+0.3
3,740,801	-				+0.3
3,741,021	-				-0.7
3,741,116	+		PP_3305	0.17	-0.4
3,741,117	-		PP_3305	0.17	-0.3
3,741,157	+		PP_3305	0.21	+0.4
3,741,198	-		PP_3305	0.25	+0.9
3,741,312	+		PP_3305	0.37	+0.1
3,741,384	+		PP_3305	0.44	-0.1
3,741,384	+		PP_3305	0.44	+0.8
3,741,384	+		PP_3305	0.44	-0.4
3,741,385	-		PP_3305	0.44	+0.8
3,741,385	-		PP_3305	0.44	-0.1
3,741,453	+		PP_3305	0.51	+0.5
3,741,453	+		PP_3305	0.51	-0.7
3,741,497	-		PP_3305	0.56	-0.9
3,741,497	-		PP_3305	0.56	+0.4
3,741,497	-		PP_3305	0.56	+0.2
3,741,548	+		PP_3305	0.61	-0.2
3,741,549	-		PP_3305	0.61	-0.2
3,741,653	+		PP_3305	0.72	-0.6
3,741,694	+		PP_3305	0.76	-0.2
3,741,694	+		PP_3305	0.76	-0.5
3,741,695	-		PP_3305	0.76	+0.9
3,741,728	-		PP_3305	0.79	-0.4
3,741,755	+		PP_3305	0.82	+0.1
3,741,763	+		PP_3305	0.83	-0.5
3,741,828	+		PP_3305	0.89	+0.1
3,741,891	-				-0.4
3,741,908	-				-0.5
3,741,914	-				+0.3
3,741,917	+				+0.2
3,741,918	-				-0.7
3,741,921	-				+0.7
3,741,946	-				-0.3
3,741,946	-				-0.0
3,741,946	-				+0.9
3,741,953	+				-0.6
3,741,966	-				+0.6
3,741,966	-				-0.1
3,741,985	-				-0.2
3,742,037	+				-0.2
3,742,151	+				+0.3
3,742,152	-				+0.4
3,742,254	+				+0.4
3,742,254	+				+0.0
3,742,255	-				+0.2
3,742,255	-				-0.0
3,742,305	+				+0.0
3,742,306	-				+0.0
3,742,310	+				+0.1
3,742,346	+				+0.4
3,742,560	+				-0.5
3,742,698	-				-0.5
3,742,698	-				-0.0
3,742,698	-				+0.4
3,742,725	+				-0.0
3,742,725	+				+1.0
3,742,725	+				-0.3
3,742,726	-				+0.2
3,742,726	-				+0.1
3,742,771	-				+0.9