Strain Fitness in Pseudomonas putida KT2440 around PP_2802

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
3,192,601	+		PP_2801	0.40	+1.4
3,192,602	-		PP_2801	0.40	-0.7
3,192,602	-		PP_2801	0.40	+0.1
3,192,641	-		PP_2801	0.42	-0.0
3,192,728	+		PP_2801	0.49	+0.1
3,192,728	+		PP_2801	0.49	+0.3
3,192,729	-		PP_2801	0.49	-0.0
3,192,741	-		PP_2801	0.49	-0.2
3,192,784	-		PP_2801	0.52	-0.9
3,192,784	-		PP_2801	0.52	-0.2
3,193,368	+				+0.0
3,193,368	+				-0.8
3,193,368	+				-0.7
3,193,369	-				+0.8
3,193,396	+				+0.0
3,193,396	+				+0.1
3,193,396	+				+0.5
3,193,539	+				-0.4
3,193,539	+				+0.1
3,193,541	+				-0.1
3,193,541	+				+0.6
3,193,541	+				-0.2
3,193,542	-				-0.6
3,194,121	+		PP_2802	0.44	+0.1
3,194,121	+		PP_2802	0.44	-0.1
3,194,136	+		PP_2802	0.45	-1.1
3,194,353	-		PP_2802	0.61	+0.5
3,194,353	-		PP_2802	0.61	-0.4
3,194,353	-		PP_2802	0.61	-0.4
3,194,628	+		PP_2802	0.82	-0.7
3,194,629	-		PP_2802	0.82	-0.7
3,194,635	-		PP_2802	0.82	-0.1
3,194,677	-		PP_2802	0.86	-0.2
3,194,677	-		PP_2802	0.86	-0.5
3,194,718	+		PP_2802	0.89	-0.7
3,194,763	+				+0.2
3,194,884	-				-0.3
3,194,884	-				-0.5
3,194,898	+				+0.1
3,194,899	-				-0.2
3,194,930	+				+0.1
3,195,225	+		PP_2803	0.29	+0.1
3,195,225	+		PP_2803	0.29	+0.2
3,195,226	-		PP_2803	0.29	-0.7
3,195,226	-		PP_2803	0.29	+1.4
3,195,435	+		PP_2803	0.47	-0.3
3,195,436	-		PP_2803	0.47	-0.0
3,195,436	-		PP_2803	0.47	+0.4
3,195,547	-		PP_2803	0.56	+0.1
3,195,685	-		PP_2803	0.68	+0.2
3,195,685	-				-1.0
3,195,685	-		PP_2803	0.68	+0.5