Strain Fitness in Pseudomonas putida KT2440 around PP_2326

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
2,652,729	-		PP_2325	0.12	+0.1
2,652,729	-		PP_2325	0.12	-0.1
2,652,803	+		PP_2325	0.20	-0.1
2,652,804	-		PP_2325	0.20	+0.3
2,652,804	-		PP_2325	0.20	-0.3
2,653,035	+		PP_2325	0.44	+0.3
2,653,035	+		PP_2325	0.44	-0.1
2,653,036	-		PP_2325	0.45	+0.2
2,653,214	+		PP_2325	0.63	-0.6
2,653,214	+		PP_2325	0.63	-0.6
2,653,273	+		PP_2325	0.70	-1.0
2,653,287	+		PP_2325	0.71	-0.3
2,653,288	-		PP_2325	0.71	+0.2
2,653,338	+		PP_2325	0.76	+0.3
2,653,339	-		PP_2325	0.76	-0.3
2,653,339	-		PP_2325	0.76	+0.2
2,653,409	+		PP_2325	0.84	+0.6
2,653,409	+		PP_2325	0.84	+0.1
2,653,409	+		PP_2325	0.84	+0.1
2,653,410	-		PP_2325	0.84	-0.4
2,653,454	+		PP_2325	0.89	-0.4
2,653,454	+		PP_2325	0.89	-0.0
2,653,518	-				-0.3
2,653,643	-				+0.5
2,653,643	-				+0.2
2,653,704	+				-0.4
2,653,704	+				+0.1
2,653,705	-				+0.1
2,653,785	+		PP_2326	0.20	+0.2
2,653,785	+		PP_2326	0.20	-0.1
2,653,786	-		PP_2326	0.20	-0.5
2,653,786	-		PP_2326	0.20	+0.3
2,653,786	-		PP_2326	0.20	-0.2
2,653,786	-		PP_2326	0.20	-0.3
2,653,786	-		PP_2326	0.20	-0.2
2,653,786	-		PP_2326	0.20	+0.4
2,653,906	+		PP_2326	0.44	-0.3
2,653,906	+		PP_2326	0.44	-0.4
2,653,907	-		PP_2326	0.44	+0.8
2,653,907	-		PP_2326	0.44	-0.0
2,653,907	-		PP_2326	0.44	+0.6
2,653,907	-		PP_2326	0.44	+0.8
2,653,907	-		PP_2326	0.44	+0.2
2,653,918	+		PP_2326	0.47	-0.4
2,654,050	+		PP_2326	0.73	-1.0
2,654,051	-		PP_2326	0.73	+0.5
2,654,087	-		PP_2326	0.81	-0.2
2,654,087	-		PP_2326	0.81	+0.4
2,654,087	-		PP_2326	0.81	-0.1
2,654,139	+				-0.9
2,654,139	+				-0.8
2,654,139	+				+0.2
2,654,140	-				+0.6
2,654,140	-				-0.2
2,654,171	+				-1.4
2,654,187	+				-1.1
2,654,222	+				-1.1
2,654,255	-				+0.5
2,654,258	+				+0.8
2,654,259	-				-0.2
2,654,260	+				-0.7
2,654,260	+				-0.2
2,654,260	+				-0.4
2,654,262	+				-0.6
2,654,311	+				+0.4
2,654,311	+				+0.5
2,654,312	-				+0.0
2,654,312	-				+0.4
2,654,312	-				+0.1
2,654,333	+				-0.0
2,654,333	+				+0.5
2,654,334	-				-0.2
2,654,390	+				+0.0
2,654,390	+				+0.1
2,654,391	-				-0.6
2,654,391	-				+0.2
2,654,392	+				+0.0
2,654,392	+				-0.4
2,654,393	-				-0.4
2,654,393	-				-0.1
2,654,801	+		PP_2327	0.50	+0.4
2,654,801	+		PP_2327	0.50	+0.4
2,654,801	+		PP_2327	0.50	-0.3
2,654,801	+		PP_2327	0.50	+0.2
2,654,801	+		PP_2327	0.50	-0.2
2,654,802	-		PP_2327	0.50	-0.0
2,654,900	+		PP_2327	0.60	-0.1
2,654,900	+		PP_2327	0.60	+0.5
2,654,900	+		PP_2327	0.60	+0.2
2,654,901	-		PP_2327	0.60	-0.4
2,654,901	-		PP_2327	0.60	+0.2
2,655,008	+		PP_2327	0.71	-0.0
2,655,008	+		PP_2327	0.71	+0.1
2,655,008	+		PP_2327	0.71	+0.1
2,655,008	+		PP_2327	0.71	+0.1
2,655,008	+		PP_2327	0.71	+0.4
2,655,009	-		PP_2327	0.71	-0.0
2,655,009	-		PP_2327	0.71	+0.2