Strain Fitness in Pseudomonas putida KT2440 around PP_4736

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_4735 and PP_4736 are separated by 62 nucleotidesPP_4736 and PP_4737 are separated by 75 nucleotides PP_4735: PP_4735 - L-lactate permease, at 5,383,537 to 5,385,207 _4735 PP_4736: PP_4736 - L-lactate dehydrogenase, at 5,385,270 to 5,386,415 _4736 PP_4737: PP_4737 - D-lactate dehydrogenase, at 5,386,491 to 5,389,301 _4737 Position (kb) 5385 5386 5387Strain fitness (log2 ratio) -1 0 1 2at 5384.425 kb on + strand, within PP_4735at 5384.426 kb on - strand, within PP_4735at 5384.539 kb on + strand, within PP_4735at 5384.539 kb on + strand, within PP_4735at 5384.539 kb on + strand, within PP_4735at 5384.539 kb on + strand, within PP_4735at 5384.540 kb on - strand, within PP_4735at 5384.730 kb on + strand, within PP_4735at 5384.815 kb on + strand, within PP_4735at 5384.815 kb on + strand, within PP_4735at 5384.816 kb on - strand, within PP_4735at 5385.026 kb on + strand, within PP_4735at 5385.027 kb on - strand, within PP_4735at 5385.176 kb on - strandat 5385.176 kb on - strandat 5385.294 kb on + strandat 5385.340 kb on - strandat 5385.519 kb on + strand, within PP_4736at 5385.519 kb on + strand, within PP_4736at 5385.520 kb on - strand, within PP_4736at 5385.520 kb on - strand, within PP_4736at 5385.654 kb on + strand, within PP_4736at 5385.654 kb on + strand, within PP_4736at 5385.654 kb on + strand, within PP_4736at 5385.654 kb on + strand, within PP_4736at 5385.654 kb on + strand, within PP_4736at 5385.654 kb on + strand, within PP_4736at 5385.654 kb on + strand, within PP_4736at 5385.654 kb on + strand, within PP_4736at 5385.654 kb on + strand, within PP_4736at 5385.655 kb on - strand, within PP_4736at 5385.655 kb on - strand, within PP_4736at 5385.655 kb on - strand, within PP_4736at 5385.655 kb on - strand, within PP_4736at 5385.655 kb on - strand, within PP_4736at 5385.655 kb on - strand, within PP_4736at 5385.655 kb on - strand, within PP_4736at 5385.655 kb on - strand, within PP_4736at 5385.655 kb on - strand, within PP_4736at 5385.655 kb on - strand, within PP_4736at 5385.655 kb on - strand, within PP_4736at 5385.658 kb on + strand, within PP_4736at 5385.658 kb on + strand, within PP_4736at 5385.658 kb on + strand, within PP_4736at 5385.659 kb on - strand, within PP_4736at 5385.659 kb on - strand, within PP_4736at 5385.898 kb on - strand, within PP_4736at 5385.898 kb on - strand, within PP_4736at 5385.927 kb on + strand, within PP_4736at 5385.927 kb on + strand, within PP_4736at 5385.927 kb on + strand, within PP_4736at 5385.928 kb on - strand, within PP_4736at 5385.928 kb on - strand, within PP_4736at 5386.246 kb on + strand, within PP_4736at 5386.246 kb on + strand, within PP_4736at 5386.246 kb on + strand, within PP_4736at 5386.247 kb on - strand, within PP_4736at 5386.269 kb on + strand, within PP_4736at 5386.269 kb on + strand, within PP_4736at 5386.270 kb on - strand, within PP_4736at 5386.270 kb on - strandat 5386.455 kb on + strandat 5386.455 kb on + strandat 5386.455 kb on + strandat 5386.455 kb on + strandat 5386.455 kb on + strandat 5386.965 kb on + strand, within PP_4737at 5386.965 kb on + strand, within PP_4737at 5386.965 kb on + strand, within PP_4737at 5386.965 kb on + strand, within PP_4737at 5386.965 kb on + strand, within PP_4737at 5386.965 kb on + strand, within PP_4737at 5386.966 kb on - strand, within PP_4737at 5386.966 kb on - strand, within PP_4737at 5387.143 kb on - strand, within PP_4737at 5387.191 kb on - strand, within PP_4737at 5387.269 kb on - strand, within PP_4737at 5387.269 kb on - strand, within PP_4737at 5387.381 kb on - strand, within PP_4737

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Per-strain Table

Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 1
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5,384,425 + PP_4735 0.53 -0.2
5,384,426 - PP_4735 0.53 -0.6
5,384,539 + PP_4735 0.60 +0.4
5,384,539 + PP_4735 0.60 +2.0
5,384,539 + PP_4735 0.60 -0.9
5,384,539 + PP_4735 0.60 -1.0
5,384,540 - PP_4735 0.60 -0.0
5,384,730 + PP_4735 0.71 -0.6
5,384,815 + PP_4735 0.76 -0.3
5,384,815 + PP_4735 0.76 -1.0
5,384,816 - PP_4735 0.77 +0.1
5,385,026 + PP_4735 0.89 -0.6
5,385,027 - PP_4735 0.89 -0.5
5,385,176 - -0.4
5,385,176 - -0.2
5,385,294 + -0.2
5,385,340 - +0.0
5,385,519 + PP_4736 0.22 -0.3
5,385,519 + PP_4736 0.22 -0.1
5,385,520 - PP_4736 0.22 +0.3
5,385,520 - PP_4736 0.22 +0.8
5,385,654 + PP_4736 0.34 +2.0
5,385,654 + PP_4736 0.34 +0.6
5,385,654 + PP_4736 0.34 -0.9
5,385,654 + PP_4736 0.34 -0.2
5,385,654 + PP_4736 0.34 -0.5
5,385,654 + PP_4736 0.34 +0.3
5,385,654 + PP_4736 0.34 -0.1
5,385,654 + PP_4736 0.34 -0.0
5,385,654 + PP_4736 0.34 +0.3
5,385,655 - PP_4736 0.34 -0.8
5,385,655 - PP_4736 0.34 +0.1
5,385,655 - PP_4736 0.34 -0.1
5,385,655 - PP_4736 0.34 -0.5
5,385,655 - PP_4736 0.34 -0.0
5,385,655 - PP_4736 0.34 -0.8
5,385,655 - PP_4736 0.34 -0.5
5,385,655 - PP_4736 0.34 -0.3
5,385,655 - PP_4736 0.34 +0.1
5,385,655 - PP_4736 0.34 -0.4
5,385,655 - PP_4736 0.34 +0.0
5,385,658 + PP_4736 0.34 -0.4
5,385,658 + PP_4736 0.34 -0.9
5,385,658 + PP_4736 0.34 -0.5
5,385,659 - PP_4736 0.34 +0.6
5,385,659 - PP_4736 0.34 +0.5
5,385,898 - PP_4736 0.55 -0.2
5,385,898 - PP_4736 0.55 -0.6
5,385,927 + PP_4736 0.57 -0.4
5,385,927 + PP_4736 0.57 -0.1
5,385,927 + PP_4736 0.57 -1.2
5,385,928 - PP_4736 0.57 -0.6
5,385,928 - PP_4736 0.57 -0.5
5,386,246 + PP_4736 0.85 +0.0
5,386,246 + PP_4736 0.85 +0.1
5,386,246 + PP_4736 0.85 +0.8
5,386,247 - PP_4736 0.85 -0.3
5,386,269 + PP_4736 0.87 -0.8
5,386,269 + PP_4736 0.87 -0.5
5,386,270 - PP_4736 0.87 -0.3
5,386,270 - +2.2
5,386,455 + +0.1
5,386,455 + +0.2
5,386,455 + -1.7
5,386,455 + -0.5
5,386,455 + -0.1
5,386,965 + PP_4737 0.17 +0.3
5,386,965 + PP_4737 0.17 -1.0
5,386,965 + PP_4737 0.17 -0.4
5,386,965 + PP_4737 0.17 +0.0
5,386,965 + PP_4737 0.17 -0.4
5,386,965 + PP_4737 0.17 -0.6
5,386,966 - PP_4737 0.17 +0.8
5,386,966 - PP_4737 0.17 +0.8
5,387,143 - PP_4737 0.23 +0.3
5,387,191 - PP_4737 0.25 +0.3
5,387,269 - PP_4737 0.28 -0.4
5,387,269 - PP_4737 0.28 -0.0
5,387,381 - PP_4737 0.32 -0.2

Or see this region's nucleotide sequence