Strain Fitness in Pseudomonas putida KT2440 around PP_0382

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0381 and PP_0382 are separated by 140 nucleotidesPP_0382 and PP_0383 are separated by 14 nucleotides PP_0381: PP_0381 - Coenzyme PQQ synthesis protein F, at 460,164 to 462,464 _0381 PP_0382: PP_0382 - 5-aminopentanamidase, at 462,605 to 463,399 _0382 PP_0383: PP_0383 - Lysine 2-monooxygenase, at 463,414 to 465,096 _0383 Position (kb) 462 463 464Strain fitness (log2 ratio) -1 0 1 2at 461.611 kb on + strand, within PP_0381at 461.612 kb on - strand, within PP_0381at 461.672 kb on + strand, within PP_0381at 461.696 kb on - strand, within PP_0381at 461.908 kb on + strand, within PP_0381at 461.908 kb on + strand, within PP_0381at 461.972 kb on - strand, within PP_0381at 462.253 kb on + strandat 462.593 kb on + strandat 462.593 kb on + strandat 462.594 kb on - strandat 462.613 kb on + strandat 462.614 kb on - strandat 462.614 kb on - strandat 462.802 kb on - strand, within PP_0382at 462.802 kb on - strand, within PP_0382at 462.810 kb on + strand, within PP_0382at 462.810 kb on + strand, within PP_0382at 462.811 kb on - strand, within PP_0382at 462.812 kb on + strand, within PP_0382at 462.812 kb on + strand, within PP_0382at 462.812 kb on + strand, within PP_0382at 462.813 kb on - strand, within PP_0382at 462.813 kb on - strand, within PP_0382at 462.813 kb on - strand, within PP_0382at 462.813 kb on - strand, within PP_0382at 462.813 kb on - strand, within PP_0382at 462.813 kb on - strand, within PP_0382at 462.819 kb on + strand, within PP_0382at 462.819 kb on + strand, within PP_0382at 462.819 kb on + strand, within PP_0382at 462.820 kb on - strand, within PP_0382at 462.820 kb on - strand, within PP_0382at 462.865 kb on + strand, within PP_0382at 462.961 kb on - strand, within PP_0382at 462.961 kb on - strand, within PP_0382at 463.120 kb on - strand, within PP_0382at 463.155 kb on + strand, within PP_0382at 463.156 kb on - strand, within PP_0382at 463.156 kb on - strand, within PP_0382at 463.383 kb on + strandat 463.383 kb on + strandat 463.384 kb on - strandat 463.384 kb on - strandat 463.384 kb on - strandat 463.634 kb on + strand, within PP_0383at 463.634 kb on + strand, within PP_0383at 463.635 kb on - strand, within PP_0383at 463.635 kb on - strand, within PP_0383at 463.635 kb on - strand, within PP_0383at 463.694 kb on + strand, within PP_0383at 463.763 kb on + strand, within PP_0383at 463.795 kb on + strand, within PP_0383at 463.899 kb on - strand, within PP_0383at 463.899 kb on - strand, within PP_0383at 463.899 kb on - strand, within PP_0383at 463.899 kb on - strand, within PP_0383at 464.026 kb on - strand, within PP_0383at 464.026 kb on - strand, within PP_0383at 464.117 kb on - strand, within PP_0383at 464.126 kb on + strand, within PP_0383at 464.126 kb on + strand, within PP_0383at 464.127 kb on - strand, within PP_0383

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Per-strain Table

Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 1
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461,611 + PP_0381 0.63 -0.2
461,612 - PP_0381 0.63 -0.1
461,672 + PP_0381 0.66 +0.1
461,696 - PP_0381 0.67 +0.3
461,908 + PP_0381 0.76 -0.9
461,908 + PP_0381 0.76 -0.3
461,972 - PP_0381 0.79 -0.5
462,253 + +0.1
462,593 + -0.8
462,593 + -0.4
462,594 - -0.1
462,613 + +0.0
462,614 - -0.3
462,614 - -0.0
462,802 - PP_0382 0.25 -0.1
462,802 - PP_0382 0.25 +0.7
462,810 + PP_0382 0.26 -0.1
462,810 + PP_0382 0.26 +0.1
462,811 - PP_0382 0.26 -0.7
462,812 + PP_0382 0.26 -0.5
462,812 + PP_0382 0.26 +1.8
462,812 + PP_0382 0.26 +0.0
462,813 - PP_0382 0.26 -1.3
462,813 - PP_0382 0.26 +0.8
462,813 - PP_0382 0.26 +0.2
462,813 - PP_0382 0.26 +0.0
462,813 - PP_0382 0.26 +0.5
462,813 - PP_0382 0.26 +0.3
462,819 + PP_0382 0.27 +0.7
462,819 + PP_0382 0.27 -0.7
462,819 + PP_0382 0.27 -0.3
462,820 - PP_0382 0.27 -0.2
462,820 - PP_0382 0.27 -0.7
462,865 + PP_0382 0.33 -0.5
462,961 - PP_0382 0.45 +0.0
462,961 - PP_0382 0.45 -0.3
463,120 - PP_0382 0.65 -0.4
463,155 + PP_0382 0.69 -0.2
463,156 - PP_0382 0.69 +0.0
463,156 - PP_0382 0.69 +0.0
463,383 + -0.3
463,383 + -0.4
463,384 - -0.4
463,384 - -0.5
463,384 - -1.2
463,634 + PP_0383 0.13 -0.5
463,634 + PP_0383 0.13 +0.3
463,635 - PP_0383 0.13 -0.1
463,635 - PP_0383 0.13 -0.3
463,635 - PP_0383 0.13 +0.1
463,694 + PP_0383 0.17 +0.1
463,763 + PP_0383 0.21 +0.2
463,795 + PP_0383 0.23 -0.1
463,899 - PP_0383 0.29 -0.2
463,899 - PP_0383 0.29 -0.1
463,899 - PP_0383 0.29 +0.3
463,899 - PP_0383 0.29 +0.5
464,026 - PP_0383 0.36 +0.4
464,026 - PP_0383 0.36 -0.1
464,117 - PP_0383 0.42 -0.5
464,126 + PP_0383 0.42 +0.1
464,126 + PP_0383 0.42 -0.3
464,127 - PP_0383 0.42 +0.5

Or see this region's nucleotide sequence