Strain Fitness in Pseudomonas putida KT2440 around PP_3535

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
4,005,088	+				-0.9
4,005,089	-				+0.7
4,005,135	+				-0.4
4,005,135	+				-0.2
4,005,135	+				-0.1
4,005,135	+				+0.8
4,005,135	+				+0.0
4,005,136	-				+0.4
4,005,136	-				-0.2
4,005,136	-				-0.5
4,005,136	-				+0.2
4,005,253	+		PP_3534	0.11	-0.0
4,005,253	+		PP_3534	0.11	+0.2
4,005,254	-		PP_3534	0.11	-0.3
4,005,254	-		PP_3534	0.11	-2.5
4,005,377	+		PP_3534	0.25	-0.1
4,005,377	+		PP_3534	0.25	-0.1
4,005,386	+		PP_3534	0.26	-0.2
4,005,387	-		PP_3534	0.26	-0.1
4,005,607	+		PP_3534	0.51	+0.4
4,005,608	-		PP_3534	0.51	-1.0
4,005,608	-		PP_3534	0.51	+0.3
4,005,608	-		PP_3534	0.51	+0.2
4,005,698	+		PP_3534	0.61	+0.4
4,005,699	-		PP_3534	0.61	-0.5
4,005,843	+		PP_3534	0.78	+0.1
4,005,843	+		PP_3534	0.78	-0.6
4,005,844	-		PP_3534	0.78	+0.1
4,006,035	-				-0.1
4,006,042	+				+0.5
4,006,042	+				+0.4
4,006,042	+				-0.9
4,006,042	+				-0.7
4,006,042	+				+0.5
4,006,043	-				-0.6
4,006,046	+				-2.0
4,006,046	+				-0.7
4,006,047	-				-0.1
4,006,078	-				-0.0
4,006,749	+		PP_3535	0.37	-1.1
4,006,749	+		PP_3535	0.37	-1.0
4,006,750	-				-0.2
4,006,750	-		PP_3535	0.37	-0.3
4,006,788	+		PP_3535	0.39	-1.4
4,006,788	+		PP_3535	0.39	-0.4
4,006,788	+		PP_3535	0.39	-0.2
4,006,788	+		PP_3535	0.39	+0.4
4,006,789	-		PP_3535	0.39	-0.3
4,006,789	-		PP_3535	0.39	-1.3
4,006,806	+		PP_3535	0.40	-0.4
4,006,807	-		PP_3535	0.40	-1.0
4,006,973	+		PP_3535	0.50	-1.5
4,007,031	+		PP_3535	0.53	-0.2
4,007,031	+		PP_3535	0.53	-0.3
4,007,032	-		PP_3535	0.53	+0.8
4,007,067	+		PP_3535	0.55	+0.0
4,007,068	-		PP_3535	0.55	+0.1
4,007,068	-		PP_3535	0.55	+0.1
4,007,068	-		PP_3535	0.55	-0.6
4,007,154	+		PP_3535	0.60	-0.1
4,007,155	-		PP_3535	0.60	-0.5
4,007,224	+		PP_3535	0.64	-1.1
4,007,263	-		PP_3535	0.66	-0.7
4,007,263	-		PP_3535	0.66	+0.3
4,007,292	+		PP_3535	0.67	-0.3
4,007,292	+		PP_3535	0.67	-1.3
4,007,293	-		PP_3535	0.67	+0.4
4,007,309	+		PP_3535	0.68	-0.2
4,007,310	-		PP_3535	0.68	-1.4
4,007,578	-		PP_3535	0.83	-0.4
4,007,578	-		PP_3535	0.83	-0.3
4,007,801	+				+0.3
4,007,802	-				-0.3
4,007,802	-				-0.3
4,007,802	-				+1.2
4,007,837	+				+0.3
4,007,837	+				-0.4
4,007,837	+				+0.3
4,007,838	-				-2.0
4,007,898	-				+0.0
4,008,084	-				+0.4
4,008,096	+				+0.1
4,008,097	-				+0.0
4,008,097	-				-0.1
4,008,115	-				-0.4
4,008,134	-				-0.6
4,008,275	-				-0.5
4,008,275	-		PP_3536	0.18	-0.4
4,008,275	-		PP_3536	0.18	+0.4
4,008,295	-		PP_3536	0.20	-0.5
4,008,313	-		PP_3536	0.21	-0.7
4,008,347	+		PP_3536	0.24	-0.0
4,008,353	+		PP_3536	0.25	+0.3
4,008,354	-		PP_3536	0.25	-0.2
4,008,502	+		PP_3536	0.39	-0.5
4,008,630	+		PP_3536	0.50	+0.7
4,008,632	+		PP_3536	0.51	+0.3
4,008,636	+		PP_3536	0.51	-0.2
4,008,713	-		PP_3536	0.58	+0.9
4,008,713	-		PP_3536	0.58	-0.4
4,008,713	-		PP_3536	0.58	-0.6
4,008,731	+		PP_3536	0.60	-0.7
4,008,731	+		PP_3536	0.60	-0.5
4,008,732	-		PP_3536	0.60	+0.2
4,008,837	+		PP_3536	0.69	+0.3
4,008,838	-		PP_3536	0.69	-0.1
4,008,838	-		PP_3536	0.69	+0.2
4,008,841	+		PP_3536	0.70	+0.4
4,008,842	-		PP_3536	0.70	-0.2
4,008,842	-		PP_3536	0.70	+0.7