Strain Fitness in Pseudomonas putida KT2440 around PP_2539

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
2,882,706	+		PP_2538	0.16	-1.0
2,882,706	+		PP_2538	0.16	-0.8
2,882,706	+		PP_2538	0.16	-0.7
2,882,706	+		PP_2538	0.16	+0.0
2,882,706	+		PP_2538	0.16	+0.1
2,882,707	-		PP_2538	0.16	+0.9
2,882,707	-		PP_2538	0.16	+0.8
2,882,712	+		PP_2538	0.17	-0.5
2,882,721	+		PP_2538	0.18	+0.7
2,882,773	-		PP_2538	0.25	-1.2
2,882,773	-		PP_2538	0.25	+0.1
2,882,865	+		PP_2538	0.38	+0.1
2,883,059	+		PP_2538	0.65	-0.4
2,883,060	-		PP_2538	0.65	+0.2
2,883,060	-		PP_2538	0.65	+0.5
2,883,103	-		PP_2538	0.71	+0.2
2,883,117	+		PP_2538	0.73	+0.3
2,883,184	-		PP_2538	0.82	-0.5
2,883,253	+				+0.1
2,883,254	-				+0.1
2,883,254	-				-0.4
2,883,268	+				-1.1
2,883,268	+				+0.4
2,883,269	-				+0.4
2,883,400	+				-0.4
2,883,400	+				+0.1
2,883,400	+				-0.2
2,883,401	-				+0.1
2,883,401	-				-0.0
2,883,401	-				+0.7
2,883,402	+				+0.1
2,883,402	+				-0.4
2,883,403	-				+0.3
2,883,403	-				-0.4
2,883,530	+				+0.5
2,883,531	-				+0.4
2,883,608	-				+2.1
2,883,672	+		PP_2539	0.25	+0.1
2,883,764	+		PP_2539	0.35	+0.1
2,883,764	+		PP_2539	0.35	-0.0
2,883,765	-		PP_2539	0.35	+0.4
2,883,777	-		PP_2539	0.36	+0.3
2,883,851	+		PP_2539	0.44	-0.1
2,884,035	-		PP_2539	0.64	+0.1
2,884,035	-		PP_2539	0.64	+0.6
2,884,035	-		PP_2539	0.64	-0.1
2,884,087	+		PP_2539	0.70	+0.0
2,884,414	-				-0.4
2,884,414	-				-0.0
2,884,472	+				+0.1
2,884,472	+				+0.8
2,884,473	-				+0.1
2,884,543	+				-0.4
2,884,543	+				-0.5
2,884,544	-				-0.2
2,884,719	-		PP_2540	0.24	-0.0
2,884,909	+		PP_2540	0.48	+0.4
2,884,910	-		PP_2540	0.48	+0.4
2,884,910	-		PP_2540	0.48	+0.1
2,884,910	-		PP_2540	0.48	+1.1
2,885,224	-		PP_2540	0.89	-0.4
2,885,257	-				-0.6
2,885,307	+				+0.6
2,885,308	-				+0.3