Strain Fitness in Pseudomonas putida KT2440 around PP_0033

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0031 and PP_0032 are separated by 266 nucleotidesPP_0032 and PP_0033 are separated by 82 nucleotidesPP_0033 and PP_0034 overlap by 7 nucleotidesPP_0034 and PP_0035 overlap by 35 nucleotides PP_0031: PP_0031 - hypothetical protein, at 35,040 to 35,627 _0031 PP_0032: PP_0032 - conserved hypothetical protein, at 35,894 to 36,115 _0032 PP_0033: PP_0033 - putative undecaprenyl-glycosyl transferase, at 36,198 to 37,652 _0033 PP_0034: PP_0034 - putative bactoprenol glycosyl-transferase from phage origin, at 37,646 to 38,629 _0034 PP_0035: PP_0035 - putative bactoprenol-linked glycose transferase, at 38,595 to 39,014 _0035 Position (kb) 36 37 38Strain fitness (log2 ratio) -2 -1 0 1at 35.257 kb on + strand, within PP_0031at 35.257 kb on + strand, within PP_0031at 35.258 kb on - strand, within PP_0031at 35.258 kb on - strandat 35.258 kb on - strand, within PP_0031at 35.297 kb on + strand, within PP_0031at 35.298 kb on - strand, within PP_0031at 35.327 kb on + strand, within PP_0031at 35.327 kb on + strand, within PP_0031at 35.328 kb on - strand, within PP_0031at 35.384 kb on - strand, within PP_0031at 35.616 kb on + strandat 35.617 kb on - strandat 35.617 kb on - strandat 35.797 kb on + strandat 35.860 kb on - strandat 35.877 kb on - strandat 35.877 kb on - strandat 36.094 kb on + strandat 36.199 kb on + strandat 36.327 kb on + strandat 36.327 kb on + strandat 36.327 kb on + strandat 36.328 kb on - strandat 36.328 kb on - strandat 36.328 kb on - strandat 36.328 kb on - strandat 36.376 kb on + strand, within PP_0033at 36.376 kb on + strand, within PP_0033at 36.376 kb on + strand, within PP_0033at 36.376 kb on + strand, within PP_0033at 36.377 kb on - strand, within PP_0033at 36.454 kb on + strand, within PP_0033at 36.455 kb on - strand, within PP_0033at 36.472 kb on + strand, within PP_0033at 36.473 kb on - strand, within PP_0033at 36.473 kb on - strand, within PP_0033at 36.473 kb on - strand, within PP_0033at 36.552 kb on + strand, within PP_0033at 36.553 kb on - strand, within PP_0033at 36.553 kb on - strand, within PP_0033at 36.553 kb on - strand, within PP_0033at 36.565 kb on + strand, within PP_0033at 36.695 kb on - strand, within PP_0033at 36.695 kb on - strand, within PP_0033at 36.695 kb on - strand, within PP_0033at 36.695 kb on - strand, within PP_0033at 36.760 kb on + strand, within PP_0033at 36.761 kb on - strand, within PP_0033at 36.761 kb on - strand, within PP_0033at 36.761 kb on - strandat 36.807 kb on + strand, within PP_0033at 36.808 kb on - strand, within PP_0033at 36.808 kb on - strand, within PP_0033at 36.828 kb on + strand, within PP_0033at 36.863 kb on - strand, within PP_0033at 36.881 kb on + strand, within PP_0033at 36.882 kb on - strand, within PP_0033at 36.882 kb on - strand, within PP_0033at 36.882 kb on - strand, within PP_0033at 36.886 kb on + strand, within PP_0033at 36.887 kb on - strand, within PP_0033at 36.887 kb on - strand, within PP_0033at 36.887 kb on - strand, within PP_0033at 36.887 kb on - strand, within PP_0033at 36.887 kb on - strand, within PP_0033at 36.887 kb on - strand, within PP_0033at 36.906 kb on - strand, within PP_0033at 36.906 kb on - strand, within PP_0033at 36.946 kb on + strand, within PP_0033at 36.946 kb on + strand, within PP_0033at 36.946 kb on + strand, within PP_0033at 36.947 kb on - strand, within PP_0033at 36.947 kb on - strand, within PP_0033at 36.947 kb on - strand, within PP_0033at 36.947 kb on - strand, within PP_0033at 36.947 kb on - strand, within PP_0033at 36.985 kb on + strand, within PP_0033at 36.985 kb on + strand, within PP_0033at 36.985 kb on + strand, within PP_0033at 36.985 kb on + strand, within PP_0033at 36.985 kb on + strand, within PP_0033at 36.985 kb on + strand, within PP_0033at 36.985 kb on + strand, within PP_0033at 36.986 kb on - strand, within PP_0033at 36.986 kb on - strand, within PP_0033at 36.986 kb on - strand, within PP_0033at 36.986 kb on - strand, within PP_0033at 36.986 kb on - strand, within PP_0033at 36.986 kb on - strand, within PP_0033at 36.986 kb on - strand, within PP_0033at 36.986 kb on - strand, within PP_0033at 36.999 kb on + strand, within PP_0033at 37.000 kb on - strand, within PP_0033at 37.001 kb on + strand, within PP_0033at 37.001 kb on + strand, within PP_0033at 37.001 kb on + strand, within PP_0033at 37.001 kb on + strand, within PP_0033at 37.002 kb on - strand, within PP_0033at 37.002 kb on - strand, within PP_0033at 37.014 kb on + strand, within PP_0033at 37.014 kb on + strand, within PP_0033at 37.014 kb on + strand, within PP_0033at 37.014 kb on + strand, within PP_0033at 37.014 kb on + strand, within PP_0033at 37.015 kb on - strand, within PP_0033at 37.015 kb on - strand, within PP_0033at 37.015 kb on - strand, within PP_0033at 37.044 kb on + strand, within PP_0033at 37.064 kb on - strand, within PP_0033at 37.064 kb on - strand, within PP_0033at 37.067 kb on + strand, within PP_0033at 37.068 kb on - strand, within PP_0033at 37.159 kb on + strand, within PP_0033at 37.205 kb on - strand, within PP_0033at 37.205 kb on - strand, within PP_0033at 37.374 kb on + strand, within PP_0033at 37.375 kb on - strand, within PP_0033at 37.473 kb on + strand, within PP_0033at 37.481 kb on - strand, within PP_0033at 37.571 kb on - strandat 37.571 kb on - strandat 37.571 kb on - strandat 37.596 kb on + strandat 37.657 kb on + strandat 37.725 kb on + strandat 37.725 kb on + strandat 37.725 kb on + strandat 37.726 kb on - strandat 37.726 kb on - strandat 37.866 kb on + strand, within PP_0034at 37.866 kb on + strand, within PP_0034at 37.867 kb on - strand, within PP_0034at 37.867 kb on - strand, within PP_0034at 37.867 kb on - strand, within PP_0034at 38.016 kb on + strand, within PP_0034at 38.033 kb on - strand, within PP_0034at 38.042 kb on + strand, within PP_0034at 38.043 kb on - strand, within PP_0034at 38.184 kb on + strand, within PP_0034at 38.184 kb on + strand, within PP_0034at 38.184 kb on + strand, within PP_0034at 38.184 kb on + strand, within PP_0034at 38.184 kb on + strand, within PP_0034at 38.185 kb on - strand, within PP_0034at 38.185 kb on - strand, within PP_0034at 38.187 kb on + strand, within PP_0034at 38.187 kb on + strand, within PP_0034at 38.188 kb on - strand, within PP_0034at 38.275 kb on - strand, within PP_0034at 38.358 kb on - strand, within PP_0034at 38.414 kb on + strand, within PP_0034at 38.414 kb on + strand, within PP_0034at 38.414 kb on + strand, within PP_0034at 38.415 kb on - strand, within PP_0034at 38.415 kb on - strand, within PP_0034at 38.464 kb on + strand, within PP_0034at 38.465 kb on - strand, within PP_0034at 38.481 kb on + strand, within PP_0034at 38.481 kb on + strand, within PP_0034at 38.585 kb on + strandat 38.618 kb on + strandat 38.618 kb on + strandat 38.619 kb on - strandat 38.619 kb on - strandat 38.619 kb on - strandat 38.619 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
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35,257 + PP_0031 0.37 +0.6
35,257 + PP_0031 0.37 -0.6
35,258 - PP_0031 0.37 -0.8
35,258 - -0.8
35,258 - PP_0031 0.37 -0.2
35,297 + PP_0031 0.44 -0.0
35,298 - PP_0031 0.44 -0.0
35,327 + PP_0031 0.49 -0.9
35,327 + PP_0031 0.49 +0.2
35,328 - PP_0031 0.49 -0.3
35,384 - PP_0031 0.59 +0.4
35,616 + -1.1
35,617 - -0.7
35,617 - -0.2
35,797 + -0.5
35,860 - -0.7
35,877 - -0.5
35,877 - -0.2
36,094 + -0.9
36,199 + +0.2
36,327 + -0.4
36,327 + -0.2
36,327 + -0.1
36,328 - +1.6
36,328 - +0.2
36,328 - +0.2
36,328 - +0.6
36,376 + PP_0033 0.12 +0.1
36,376 + PP_0033 0.12 -0.1
36,376 + PP_0033 0.12 -0.5
36,376 + PP_0033 0.12 +0.6
36,377 - PP_0033 0.12 +0.0
36,454 + PP_0033 0.18 -0.2
36,455 - PP_0033 0.18 -0.2
36,472 + PP_0033 0.19 -1.0
36,473 - PP_0033 0.19 -0.1
36,473 - PP_0033 0.19 -0.5
36,473 - PP_0033 0.19 -0.2
36,552 + PP_0033 0.24 +0.3
36,553 - PP_0033 0.24 +0.2
36,553 - PP_0033 0.24 -1.1
36,553 - PP_0033 0.24 -0.6
36,565 + PP_0033 0.25 -0.3
36,695 - PP_0033 0.34 +0.6
36,695 - PP_0033 0.34 +0.1
36,695 - PP_0033 0.34 -0.4
36,695 - PP_0033 0.34 -0.4
36,760 + PP_0033 0.39 -0.5
36,761 - PP_0033 0.39 +0.1
36,761 - PP_0033 0.39 +0.3
36,761 - -1.4
36,807 + PP_0033 0.42 -0.2
36,808 - PP_0033 0.42 -0.3
36,808 - PP_0033 0.42 +0.5
36,828 + PP_0033 0.43 -0.8
36,863 - PP_0033 0.46 -0.2
36,881 + PP_0033 0.47 -0.7
36,882 - PP_0033 0.47 +0.4
36,882 - PP_0033 0.47 +1.2
36,882 - PP_0033 0.47 +0.2
36,886 + PP_0033 0.47 -0.7
36,887 - PP_0033 0.47 -0.1
36,887 - PP_0033 0.47 +1.4
36,887 - PP_0033 0.47 +0.1
36,887 - PP_0033 0.47 -0.6
36,887 - PP_0033 0.47 -1.8
36,887 - PP_0033 0.47 -0.1
36,906 - PP_0033 0.49 +0.6
36,906 - PP_0033 0.49 -0.4
36,946 + PP_0033 0.51 -0.0
36,946 + PP_0033 0.51 +0.4
36,946 + PP_0033 0.51 -0.0
36,947 - PP_0033 0.51 +0.1
36,947 - PP_0033 0.51 +0.7
36,947 - PP_0033 0.51 -0.8
36,947 - PP_0033 0.51 -0.1
36,947 - PP_0033 0.51 -0.8
36,985 + PP_0033 0.54 +0.1
36,985 + PP_0033 0.54 -0.4
36,985 + PP_0033 0.54 +1.2
36,985 + PP_0033 0.54 -0.2
36,985 + PP_0033 0.54 -0.6
36,985 + PP_0033 0.54 -0.4
36,985 + PP_0033 0.54 +0.2
36,986 - PP_0033 0.54 -0.0
36,986 - PP_0033 0.54 +0.1
36,986 - PP_0033 0.54 -0.2
36,986 - PP_0033 0.54 -0.5
36,986 - PP_0033 0.54 +0.0
36,986 - PP_0033 0.54 -0.7
36,986 - PP_0033 0.54 -0.5
36,986 - PP_0033 0.54 -0.4
36,999 + PP_0033 0.55 +0.3
37,000 - PP_0033 0.55 -0.2
37,001 + PP_0033 0.55 -0.4
37,001 + PP_0033 0.55 -0.6
37,001 + PP_0033 0.55 -0.6
37,001 + PP_0033 0.55 +0.3
37,002 - PP_0033 0.55 -0.6
37,002 - PP_0033 0.55 -0.1
37,014 + PP_0033 0.56 +0.5
37,014 + PP_0033 0.56 +0.0
37,014 + PP_0033 0.56 +0.1
37,014 + PP_0033 0.56 +0.3
37,014 + PP_0033 0.56 -0.1
37,015 - PP_0033 0.56 -0.3
37,015 - PP_0033 0.56 -0.5
37,015 - PP_0033 0.56 -0.5
37,044 + PP_0033 0.58 -0.2
37,064 - PP_0033 0.60 +0.0
37,064 - PP_0033 0.60 -0.2
37,067 + PP_0033 0.60 -0.3
37,068 - PP_0033 0.60 -1.0
37,159 + PP_0033 0.66 +0.1
37,205 - PP_0033 0.69 +0.6
37,205 - PP_0033 0.69 -0.1
37,374 + PP_0033 0.81 -0.4
37,375 - PP_0033 0.81 -0.2
37,473 + PP_0033 0.88 -0.3
37,481 - PP_0033 0.88 -0.5
37,571 - +0.3
37,571 - -0.8
37,571 - -0.3
37,596 + -0.1
37,657 + +0.2
37,725 + +0.0
37,725 + +0.1
37,725 + +0.1
37,726 - -1.0
37,726 - -0.7
37,866 + PP_0034 0.22 -0.9
37,866 + PP_0034 0.22 +0.0
37,867 - PP_0034 0.22 -1.2
37,867 - PP_0034 0.22 -0.7
37,867 - PP_0034 0.22 +0.2
38,016 + PP_0034 0.38 -0.4
38,033 - PP_0034 0.39 +0.2
38,042 + PP_0034 0.40 -0.1
38,043 - PP_0034 0.40 -0.4
38,184 + PP_0034 0.55 -0.8
38,184 + PP_0034 0.55 -1.0
38,184 + PP_0034 0.55 -0.2
38,184 + PP_0034 0.55 -0.6
38,184 + PP_0034 0.55 +0.7
38,185 - PP_0034 0.55 -0.2
38,185 - PP_0034 0.55 +0.2
38,187 + PP_0034 0.55 -0.5
38,187 + PP_0034 0.55 +0.3
38,188 - PP_0034 0.55 -1.1
38,275 - PP_0034 0.64 -0.5
38,358 - PP_0034 0.72 -0.5
38,414 + PP_0034 0.78 -0.9
38,414 + PP_0034 0.78 -0.8
38,414 + PP_0034 0.78 -0.2
38,415 - PP_0034 0.78 -1.2
38,415 - PP_0034 0.78 -0.4
38,464 + PP_0034 0.83 +0.7
38,465 - PP_0034 0.83 -0.6
38,481 + PP_0034 0.85 -0.2
38,481 + PP_0034 0.85 +0.0
38,585 + -0.0
38,618 + +0.1
38,618 + -0.5
38,619 - -0.1
38,619 - -0.5
38,619 - +0.2
38,619 - -0.3

Or see this region's nucleotide sequence