Strain Fitness in Pseudomonas putida KT2440 around PP_5549

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
3,403,026	-				+0.4
3,403,089	+		PP_3012	0.37	-0.1
3,403,089	+		PP_3012	0.37	+0.3
3,403,089	+		PP_3012	0.37	+0.1
3,403,089	+		PP_3012	0.37	-0.4
3,403,090	-		PP_3012	0.38	+0.1
3,403,090	-		PP_3012	0.38	-0.6
3,403,132	+		PP_3012	0.71	+0.1
3,403,132	+		PP_3012	0.71	+0.2
3,403,133	-		PP_3012	0.71	+1.0
3,403,133	-		PP_3012	0.71	-0.1
3,403,133	-		PP_3012	0.71	-0.3
3,403,229	-				-1.1
3,403,309	+		PP_3013	0.38	+0.1
3,403,309	+		PP_3013	0.38	-0.3
3,403,309	+		PP_3013	0.38	+0.3
3,403,310	-		PP_3013	0.38	-0.1
3,403,332	+		PP_3013	0.47	+0.1
3,403,333	-		PP_3013	0.48	+0.1
3,403,333	-		PP_3013	0.48	-0.7
3,403,354	-				-0.7
3,403,380	-		PP_3013	0.66	+0.0
3,403,484	+				-0.7
3,403,485	-				-0.0
3,403,496	+				+0.5
3,403,496	+				+0.3
3,403,559	+				+0.1
3,403,591	+				-0.1
3,403,640	-		PP_3014	0.16	+0.5
3,403,725	-		PP_3014	0.39	+1.8
3,403,880	+		PP_3014	0.82	+0.0
3,403,880	+		PP_3014	0.82	-0.4
3,403,881	-		PP_3014	0.83	-0.1
3,403,912	-				-0.1
3,403,912	-				+0.0
3,403,912	-				+0.4
3,403,986	+				-0.1
3,404,049	+		PP_5549	0.31	+0.5
3,404,050	-		PP_5549	0.31	+0.3
3,404,085	+		PP_5549	0.48	-0.2
3,404,085	+		PP_5549	0.48	+0.5
3,404,085	+		PP_5549	0.48	-0.4
3,404,118	+		PP_5549	0.64	-0.4
3,404,119	-		PP_5549	0.65	-0.3
3,404,119	-		PP_5549	0.65	+0.0
3,404,121	+		PP_5549	0.66	-0.8
3,404,218	+				-0.2
3,404,218	+				-1.0
3,404,218	+				-0.1
3,404,219	-				-0.2
3,404,222	+				-0.3
3,404,222	+				+0.3
3,404,222	+				+0.4
3,404,222	+				-0.2
3,404,223	-				-0.8
3,404,223	-				-0.9
3,404,225	+				-1.2
3,404,225	+				-0.0
3,404,225	+				+0.2
3,404,226	-				-0.0
3,404,240	+				-0.7
3,404,241	-				+0.6
3,404,241	-				-0.6
3,404,298	+				-0.1
3,404,511	+				+0.4
3,404,606	-				+1.8
3,404,611	+				-0.2
3,404,615	+				-0.0
3,404,615	+				-1.0
3,404,632	+				-0.7
3,404,632	+				-0.3
3,404,633	-				-0.6
3,404,634	+				-0.2
3,404,634	+				+0.5
3,404,634	+				-0.7
3,404,635	-				-1.1
3,404,646	+				+0.8
3,404,646	+				-0.4
3,404,646	+				-0.6
3,404,647	-				-1.0
3,404,647	-				-0.2
3,404,658	+				-0.3
3,404,659	-				-1.4
3,404,659	-				-0.9
3,404,893	+		PP_3016	0.29	+0.6
3,404,893	+		PP_3016	0.29	+0.1
3,404,893	+		PP_3016	0.29	-2.4
3,404,893	+		PP_3016	0.29	-0.1
3,404,894	-		PP_3016	0.29	+0.6
3,404,894	-		PP_3016	0.29	-0.3
3,404,894	-		PP_3016	0.29	-0.8
3,404,894	-		PP_3016	0.29	+0.2
3,404,989	+		PP_3016	0.41	+0.5
3,405,025	+		PP_3016	0.45	-0.6
3,405,025	+		PP_3016	0.45	-0.7
3,405,184	+		PP_3016	0.64	+0.3
3,405,184	+		PP_3016	0.64	-1.2
3,405,184	+		PP_3016	0.64	+0.1
3,405,184	+		PP_3016	0.64	+0.2
3,405,184	+		PP_3016	0.64	+0.4
3,405,185	-		PP_3016	0.64	-0.3
3,405,185	-		PP_3016	0.64	-0.4
3,405,185	-		PP_3016	0.64	-0.1
3,405,185	-		PP_3016	0.64	-0.9
3,405,185	-		PP_3016	0.64	+0.7
3,405,185	-		PP_3016	0.64	-0.0