Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2940 and PP_5541 are separated by 591 nucleotides PP_5541 and PP_5542 are separated by 74 nucleotides PP_5542 and PP_2941 are separated by 71 nucleotides PP_2941 and PP_2942 are separated by 0 nucleotides
PP_2940: PP_2940 - antitoxin of the YoeB-YefM toxin-antitoxin pair and DNA binding transcriptional repressor, at 3,342,722 to 3,343,006
_2940
PP_5541: PP_5541 - conserved protein of unknown function, at 3,343,598 to 3,343,927
_5541
PP_5542: PP_5542 - protein of unknown function, at 3,344,002 to 3,344,271
_5542
PP_2941: PP_2941 - conserved protein of unknown function, at 3,344,343 to 3,345,128
_2941
PP_2942: PP_2942 - Response regulator, at 3,345,129 to 3,346,499
_2942
Position (kb)
3344
3345 Strain fitness (log2 ratio)
-1
0
1
2 at 3343.226 kb on - strand at 3343.269 kb on + strand at 3343.269 kb on + strand at 3343.270 kb on - strand at 3343.302 kb on + strand at 3343.303 kb on - strand at 3343.338 kb on - strand at 3343.339 kb on + strand at 3343.340 kb on - strand at 3343.486 kb on + strand at 3343.486 kb on + strand at 3343.504 kb on - strand at 3343.525 kb on + strand at 3343.525 kb on + strand at 3343.555 kb on - strand at 3343.586 kb on + strand at 3343.659 kb on - strand, within PP_5541 at 3343.741 kb on + strand, within PP_5541 at 3343.741 kb on + strand, within PP_5541 at 3343.746 kb on - strand, within PP_5541 at 3343.779 kb on + strand, within PP_5541 at 3343.780 kb on - strand, within PP_5541 at 3343.780 kb on - strand, within PP_5541 at 3343.780 kb on - strand, within PP_5541 at 3343.970 kb on + strand at 3343.970 kb on + strand at 3343.971 kb on - strand at 3344.045 kb on - strand, within PP_5542 at 3344.045 kb on - strand, within PP_5542 at 3344.056 kb on + strand, within PP_5542 at 3344.057 kb on - strand, within PP_5542 at 3344.066 kb on - strand, within PP_5542 at 3344.133 kb on - strand, within PP_5542 at 3344.231 kb on - strand, within PP_5542 at 3344.231 kb on - strand, within PP_5542 at 3344.410 kb on + strand at 3344.411 kb on - strand at 3344.545 kb on - strand, within PP_2941 at 3344.545 kb on - strand, within PP_2941 at 3344.656 kb on + strand, within PP_2941 at 3344.656 kb on + strand, within PP_2941 at 3344.657 kb on - strand, within PP_2941 at 3344.657 kb on - strand, within PP_2941 at 3344.660 kb on - strand, within PP_2941 at 3344.660 kb on - strand, within PP_2941 at 3344.698 kb on + strand, within PP_2941 at 3344.699 kb on - strand, within PP_2941 at 3344.903 kb on - strand, within PP_2941 at 3344.977 kb on - strand, within PP_2941 at 3345.035 kb on + strand, within PP_2941 at 3345.162 kb on + strand at 3345.164 kb on + strand at 3345.164 kb on + strand at 3345.164 kb on + strand at 3345.164 kb on + strand at 3345.165 kb on - strand at 3345.165 kb on - strand at 3345.203 kb on + strand at 3345.203 kb on + strand at 3345.203 kb on + strand at 3345.203 kb on + strand at 3345.204 kb on - strand at 3345.204 kb on - strand at 3345.204 kb on - strand at 3345.204 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1 remove 3,343,226 - +0.6 3,343,269 + +0.3 3,343,269 + -0.2 3,343,270 - +0.0 3,343,302 + +0.5 3,343,303 - -0.8 3,343,338 - -0.6 3,343,339 + -0.1 3,343,340 - +0.4 3,343,486 + +0.4 3,343,486 + -0.3 3,343,504 - -1.1 3,343,525 + +0.0 3,343,525 + -0.8 3,343,555 - -0.5 3,343,586 + +0.1 3,343,659 - PP_5541 0.18 +0.2 3,343,741 + PP_5541 0.43 +0.2 3,343,741 + PP_5541 0.43 -0.2 3,343,746 - PP_5541 0.45 -0.6 3,343,779 + PP_5541 0.55 +0.1 3,343,780 - PP_5541 0.55 +0.0 3,343,780 - PP_5541 0.55 -0.4 3,343,780 - PP_5541 0.55 +0.2 3,343,970 + -0.3 3,343,970 + +0.2 3,343,971 - +1.3 3,344,045 - PP_5542 0.16 +0.1 3,344,045 - PP_5542 0.16 -0.3 3,344,056 + PP_5542 0.20 -0.2 3,344,057 - PP_5542 0.20 -0.7 3,344,066 - PP_5542 0.24 +0.7 3,344,133 - PP_5542 0.49 -1.0 3,344,231 - PP_5542 0.85 -1.4 3,344,231 - PP_5542 0.85 -0.9 3,344,410 + -0.2 3,344,411 - +0.4 3,344,545 - PP_2941 0.26 +0.1 3,344,545 - PP_2941 0.26 -0.2 3,344,656 + PP_2941 0.40 +0.0 3,344,656 + PP_2941 0.40 +0.2 3,344,657 - PP_2941 0.40 -0.4 3,344,657 - PP_2941 0.40 -0.8 3,344,660 - PP_2941 0.40 +0.2 3,344,660 - PP_2941 0.40 +0.0 3,344,698 + PP_2941 0.45 +0.2 3,344,699 - PP_2941 0.45 +0.3 3,344,903 - PP_2941 0.71 +0.1 3,344,977 - PP_2941 0.81 +0.7 3,345,035 + PP_2941 0.88 -0.3 3,345,162 + +0.6 3,345,164 + +0.4 3,345,164 + -0.3 3,345,164 + -0.1 3,345,164 + +0.3 3,345,165 - +0.0 3,345,165 - -0.2 3,345,203 + +0.3 3,345,203 + -0.3 3,345,203 + -0.2 3,345,203 + -0.1 3,345,204 - +0.1 3,345,204 - -0.9 3,345,204 - +1.8 3,345,204 - +1.3
Or see this region's nucleotide sequence