Strain Fitness in Pseudomonas putida KT2440 around PP_3923

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
4,428,053	+		PP_3922	0.30	+0.2
4,428,053	+		PP_3922	0.30	+0.5
4,428,054	-		PP_3922	0.30	+0.3
4,428,107	+		PP_3922	0.35	-0.5
4,428,192	+		PP_3922	0.44	-0.4
4,428,192	+		PP_3922	0.44	-0.9
4,428,192	+		PP_3922	0.44	+0.0
4,428,192	+		PP_3922	0.44	-0.2
4,428,192	+		PP_3922	0.44	-0.2
4,428,192	+		PP_3922	0.44	-0.6
4,428,192	+		PP_3922	0.44	-0.6
4,428,193	-		PP_3922	0.44	-0.6
4,428,193	-		PP_3922	0.44	-0.2
4,428,193	-		PP_3922	0.44	-0.1
4,428,193	-		PP_3922	0.44	+0.5
4,428,251	+		PP_3922	0.50	-0.2
4,428,251	+		PP_3922	0.50	-0.5
4,428,252	-		PP_3922	0.50	-0.3
4,428,341	+		PP_3922	0.58	+0.6
4,428,341	+		PP_3922	0.58	+0.5
4,428,478	+		PP_3922	0.72	-0.6
4,428,637	+		PP_3922	0.87	+0.8
4,428,638	-		PP_3922	0.87	-0.0
4,428,638	-		PP_3922	0.87	+0.1
4,428,638	-		PP_3922	0.87	-0.5
4,428,746	+				-0.0
4,428,776	+				-0.7
4,428,777	-				+0.7
4,428,816	+				+0.4
4,428,817	-				-0.4
4,428,818	-				-0.3
4,428,975	+				-0.1
4,429,017	+				+0.2
4,429,018	-				+0.1
4,429,024	+				+0.2
4,429,024	+				+0.8
4,429,025	-				-0.2
4,429,411	+		PP_3923	0.63	+0.4
4,429,411	+		PP_3923	0.63	-1.1
4,429,412	-		PP_3923	0.63	+0.3
4,429,466	+		PP_3923	0.71	+0.0
4,429,466	+		PP_3923	0.71	+0.5
4,429,467	-		PP_3923	0.71	+0.2
4,429,467	-		PP_3923	0.71	+0.4
4,429,467	-		PP_3923	0.71	+1.0
4,429,467	-		PP_3923	0.71	+0.4
4,429,467	-		PP_3923	0.71	-0.6
4,429,467	-		PP_3923	0.71	-0.1
4,429,517	+		PP_3923	0.78	-1.6
4,429,518	-		PP_3923	0.78	+0.6
4,429,518	-		PP_3923	0.78	-0.0
4,429,521	-		PP_3923	0.78	+0.8
4,429,665	+				-0.6
4,429,705	+				-0.3
4,429,706	-				-0.1
4,429,715	+				-0.5
4,429,715	+				+0.2
4,429,715	+				-0.3
4,429,716	-				+0.7
4,429,716	-				-0.8
4,429,716	-				+0.9
4,429,841	+		PP_3924	0.35	-0.8
4,429,842	-		PP_3924	0.35	-0.1
4,429,940	-		PP_3924	0.66	-0.4
4,429,944	+		PP_3924	0.67	-0.0
4,429,944	+		PP_3924	0.67	+0.7
4,429,945	-		PP_3924	0.68	+0.3
4,429,945	-		PP_3924	0.68	+0.4
4,429,945	-		PP_3924	0.68	-1.4
4,430,146	+				+0.5
4,430,146	+				-0.8
4,430,147	-				-0.1
4,430,147	-				+0.5
4,430,147	-				+0.0
4,430,147	-				-0.5
4,430,147	-				+0.4
4,430,189	-				+0.2
4,430,189	-				+0.1
4,430,193	+				+1.2
4,430,200	+				-0.9
4,430,300	+				-0.7
4,430,301	-				+0.6
4,430,379	-		PP_3925	0.14	-0.2
4,430,379	-		PP_3925	0.14	-0.6
4,430,391	-		PP_3925	0.15	-0.2
4,430,561	+		PP_3925	0.31	-0.1
4,430,561	+		PP_3925	0.31	-0.5
4,430,561	+		PP_3925	0.31	+0.3
4,430,561	+		PP_3925	0.31	-0.2
4,430,562	-		PP_3925	0.31	+0.0