Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2791 and PP_2792 overlap by 4 nucleotides PP_2792 and PP_2793 are separated by 53 nucleotides PP_2793 and PP_2794 overlap by 1 nucleotides PP_2794 and PP_2795 are separated by 83 nucleotides
PP_2791: PP_2791 - 4-hydroxypentanoyl-CoA kinase, subunit LvaA (from data) , at 3,180,804 to 3,181,934
_2791
PP_2792: PP_2792 - conserved protein of unknown function, at 3,181,931 to 3,182,287
_2792
PP_2793: PP_2793 - Acyl-CoA dehydrogenase family protein, at 3,182,341 to 3,183,513
_2793
PP_2794: PP_2794 - Oxidoreductase, short chain dehydrogenase/reductase family, at 3,183,513 to 3,184,280
_2794
PP_2795: PP_2795 - AMP-binding domain protein, at 3,184,364 to 3,186,010
_2795
Position (kb)
3182
3183
3184 Strain fitness (log2 ratio)
-1
0
1 at 3181.560 kb on + strand, within PP_2791 at 3181.771 kb on - strand, within PP_2791 at 3181.861 kb on + strand at 3181.861 kb on + strand at 3181.862 kb on - strand at 3182.318 kb on + strand at 3182.617 kb on - strand, within PP_2793 at 3183.009 kb on + strand, within PP_2793 at 3183.056 kb on - strand, within PP_2793 at 3183.439 kb on + strand at 3183.439 kb on + strand at 3183.820 kb on + strand, within PP_2794 at 3183.988 kb on - strand, within PP_2794 at 3184.084 kb on - strand, within PP_2794 at 3184.278 kb on + strand at 3184.297 kb on - strand at 3184.315 kb on + strand at 3184.319 kb on + strand at 3184.319 kb on + strand at 3184.320 kb on - strand at 3184.320 kb on - strand at 3184.320 kb on - strand at 3184.320 kb on - strand at 3184.320 kb on - strand at 3184.320 kb on - strand at 3184.320 kb on - strand at 3184.320 kb on - strand at 3184.320 kb on - strand at 3184.460 kb on + strand at 3184.484 kb on + strand at 3184.485 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1 remove 3,181,560 + PP_2791 0.67 -0.5 3,181,771 - PP_2791 0.85 +0.4 3,181,861 + +0.2 3,181,861 + +0.0 3,181,862 - -0.4 3,182,318 + +0.0 3,182,617 - PP_2793 0.24 -0.1 3,183,009 + PP_2793 0.57 +0.9 3,183,056 - PP_2793 0.61 -0.5 3,183,439 + +0.1 3,183,439 + +0.8 3,183,820 + PP_2794 0.40 +1.1 3,183,988 - PP_2794 0.62 +0.1 3,184,084 - PP_2794 0.74 -0.3 3,184,278 + -0.2 3,184,297 - +0.5 3,184,315 + +0.0 3,184,319 + +0.7 3,184,319 + -0.2 3,184,320 - -0.1 3,184,320 - -0.3 3,184,320 - +0.3 3,184,320 - -0.9 3,184,320 - +0.1 3,184,320 - -0.1 3,184,320 - -0.1 3,184,320 - +0.9 3,184,320 - -0.9 3,184,460 + -0.2 3,184,484 + +0.2 3,184,485 - +0.1
Or see this region's nucleotide sequence