Strain Fitness in Pseudomonas putida KT2440 around PP_2457

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
2,803,246	-				-0.1
2,803,263	+				-0.1
2,803,264	-				-0.8
2,803,264	-				+0.6
2,803,335	+		PP_2456	0.15	+0.0
2,803,336	-		PP_2456	0.15	-0.4
2,803,636	-		PP_2456	0.45	+0.4
2,803,662	+		PP_2456	0.48	-0.8
2,803,662	+		PP_2456	0.48	+0.5
2,803,662	+		PP_2456	0.48	-0.7
2,803,663	-		PP_2456	0.48	-0.0
2,803,663	-		PP_2456	0.48	+0.7
2,803,762	+		PP_2456	0.58	-1.4
2,803,851	+		PP_2456	0.67	+0.1
2,803,852	-		PP_2456	0.67	-0.2
2,804,152	-				-0.4
2,804,156	+				-0.1
2,804,230	+				-1.0
2,804,230	+				-0.9
2,804,249	+				+0.1
2,804,249	+				-0.8
2,804,249	+				+1.3
2,804,250	-				-0.1
2,804,250	-				-0.1
2,804,250	-				-0.1
2,804,250	-				+1.0
2,804,456	-		PP_2457	0.26	+0.9
2,804,507	-		PP_2457	0.31	-0.5
2,804,546	+		PP_2457	0.35	-0.4
2,804,680	+		PP_2457	0.48	+0.6
2,804,681	-		PP_2457	0.48	-0.1
2,804,681	-		PP_2457	0.48	-0.3
2,804,728	+		PP_2457	0.53	-1.0
2,804,729	-		PP_2457	0.53	-0.8
2,804,730	+		PP_2457	0.53	+0.2
2,804,730	-		PP_2457	0.53	-0.4
2,804,828	+		PP_2457	0.63	-0.8
2,804,828	+		PP_2457	0.63	-0.0
2,804,829	-				+2.0
2,804,972	+		PP_2457	0.77	-0.1
2,805,022	+		PP_2457	0.82	+0.0
2,805,023	-		PP_2457	0.82	-0.2
2,805,023	-		PP_2457	0.82	-0.0
2,805,396	+		PP_2458	0.19	-0.3
2,805,396	+		PP_2458	0.19	+0.5
2,805,424	+				+3.6
2,805,425	-		PP_2458	0.22	-0.3
2,805,451	+		PP_2458	0.25	-0.4
2,805,451	+		PP_2458	0.25	-0.2
2,805,451	+		PP_2458	0.25	+0.2
2,805,451	+		PP_2458	0.25	-0.8
2,805,452	-		PP_2458	0.25	+0.1
2,805,452	-		PP_2458	0.25	+0.2
2,805,452	-		PP_2458	0.25	+0.1
2,805,452	-		PP_2458	0.25	+0.2
2,805,546	+		PP_2458	0.35	-0.6
2,805,546	+		PP_2458	0.35	+0.5
2,805,546	+		PP_2458	0.35	-0.5
2,805,546	+		PP_2458	0.35	+0.2
2,805,547	-		PP_2458	0.35	-0.0
2,805,547	-		PP_2458	0.35	-0.0
2,805,547	-		PP_2458	0.35	+0.0
2,805,629	+		PP_2458	0.44	+0.1
2,805,629	+		PP_2458	0.44	+0.2
2,805,630	-		PP_2458	0.44	+0.1
2,805,630	-		PP_2458	0.44	-1.0
2,805,630	-		PP_2458	0.44	-0.3
2,805,630	-		PP_2458	0.44	+0.1
2,805,919	-		PP_2458	0.76	-0.2
2,805,940	+		PP_2458	0.79	-0.3
2,805,987	+		PP_2458	0.84	-0.3