Strain Fitness in Pseudomonas putida KT2440 around PP_1954

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
2,209,617	+				-0.3
2,209,621	+				+0.0
2,209,622	-				+0.6
2,209,738	-				-0.6
2,209,738	-				-0.1
2,209,829	+				+0.2
2,209,829	+				+0.6
2,209,829	+				-0.3
2,209,830	-				+1.1
2,209,830	-				-0.7
2,209,866	+		PP_1953	0.14	+0.4
2,209,866	+		PP_1953	0.14	+0.3
2,209,867	-		PP_1953	0.14	-0.5
2,210,001	+		PP_1953	0.30	-0.4
2,210,002	-		PP_1953	0.30	+0.6
2,210,002	-		PP_1953	0.30	-0.2
2,210,002	-		PP_1953	0.30	+0.5
2,210,124	+		PP_1953	0.45	+0.0
2,210,124	+		PP_1953	0.45	-0.0
2,210,281	+		PP_1953	0.65	+0.7
2,210,281	+		PP_1953	0.65	-0.4
2,210,281	+		PP_1953	0.65	+0.5
2,210,282	-		PP_1953	0.65	+0.1
2,210,282	-		PP_1953	0.65	-0.9
2,210,282	-		PP_1953	0.65	+0.1
2,210,282	-		PP_1953	0.65	-0.4
2,210,285	-		PP_1953	0.65	-0.4
2,210,347	+		PP_1953	0.73	-0.1
2,210,348	-		PP_1953	0.73	-1.0
2,210,348	-		PP_1953	0.73	+0.0
2,210,348	-		PP_1953	0.73	+0.5
2,210,348	-		PP_1953	0.73	-0.3
2,210,348	-		PP_1953	0.73	+0.3
2,210,348	-		PP_1953	0.73	+1.2
2,210,348	-		PP_1953	0.73	+0.2
2,210,563	+				-0.3
2,210,564	-				-0.9
2,210,564	-				-0.3
2,210,564	-				+0.6
2,210,564	-				-1.3
2,210,564	-				+0.6
2,210,585	+				+0.2
2,210,585	+				-0.4
2,210,585	+				-0.8
2,210,586	-				-0.5
2,210,586	-				-0.8
2,210,587	+				-0.4
2,210,587	+				-0.1
2,210,587	+				+0.8
2,210,587	+				+0.1
2,210,595	+				-0.4
2,210,595	+				+0.8
2,210,596	-				+0.1
2,210,596	-				+0.3
2,210,615	+				-0.6
2,210,691	+				+1.0
2,210,691	+				+0.1
2,210,692	-				-0.6
2,210,692	-				-1.3
2,210,692	-				-0.1
2,210,692	-				-0.6
2,210,866	+		PP_1954	0.22	-0.2
2,210,866	+		PP_1954	0.22	+0.5
2,210,867	-		PP_1954	0.22	+0.1
2,210,867	-		PP_1954	0.22	+1.4
2,210,867	-		PP_1954	0.22	+0.2
2,210,867	-		PP_1954	0.22	+0.2
2,210,879	+		PP_1954	0.23	+0.2
2,210,896	+		PP_1954	0.25	+0.2
2,210,896	+		PP_1954	0.25	+0.0
2,210,896	+		PP_1954	0.25	-0.6
2,210,897	-		PP_1954	0.25	-0.2
2,210,897	-		PP_1954	0.25	-0.1
2,210,897	-		PP_1954	0.25	-0.5
2,211,067	+				+0.4
2,211,068	-		PP_1954	0.39	-0.2
2,211,092	+		PP_1954	0.41	-0.3
2,211,092	+		PP_1954	0.41	-0.5
2,211,093	-		PP_1954	0.41	-0.4
2,211,093	-		PP_1954	0.41	-0.6
2,211,124	+		PP_1954	0.44	-0.3
2,211,124	+		PP_1954	0.44	-0.7
2,211,124	+		PP_1954	0.44	-1.2
2,211,124	+		PP_1954	0.44	-0.2
2,211,125	-		PP_1954	0.44	+0.4
2,211,125	-		PP_1954	0.44	+0.5
2,211,125	-		PP_1954	0.44	+0.3
2,211,203	+		PP_1954	0.51	-1.3
2,211,203	+		PP_1954	0.51	+0.7
2,211,291	-		PP_1954	0.58	+0.6
2,211,291	-		PP_1954	0.58	-0.5
2,211,382	+		PP_1954	0.66	+0.1
2,211,382	+		PP_1954	0.66	+0.3
2,211,382	+		PP_1954	0.66	-0.4
2,211,382	+		PP_1954	0.66	-0.4
2,211,382	+		PP_1954	0.66	-0.5
2,211,382	+		PP_1954	0.66	-0.2
2,211,383	-		PP_1954	0.66	-0.5
2,211,383	-		PP_1954	0.66	-0.5
2,211,451	+		PP_1954	0.72	-0.2
2,211,464	+		PP_1954	0.73	-0.2
2,211,464	+		PP_1954	0.73	-0.3
2,211,465	-		PP_1954	0.73	-0.6
2,211,465	-		PP_1954	0.73	-0.2
2,211,472	+		PP_1954	0.74	-0.2
2,211,472	+		PP_1954	0.74	-0.2
2,211,473	-		PP_1954	0.74	-0.4
2,211,473	-		PP_1954	0.74	+0.0
2,211,501	+				+0.8
2,211,511	+		PP_1954	0.77	-0.5
2,211,511	+		PP_1954	0.77	+0.0
2,211,512	-		PP_1954	0.77	-0.5
2,211,512	-		PP_1954	0.77	-0.5
2,211,512	-		PP_1954	0.77	+0.2
2,211,812	-				-0.0
2,211,815	+				-0.9
2,211,876	+				-0.2
2,211,986	+				+1.0
2,211,986	+				-0.2
2,211,996	+		PP_1955	0.10	+0.0
2,212,072	+		PP_1955	0.16	+0.1
2,212,073	-		PP_1955	0.17	-0.0
2,212,298	+		PP_1955	0.35	-0.7
2,212,299	-		PP_1955	0.35	+0.1
2,212,338	-		PP_1955	0.38	+0.4
2,212,376	-		PP_1955	0.41	-0.0
2,212,412	+		PP_1955	0.44	-0.9
2,212,413	-		PP_1955	0.44	-0.0
2,212,413	-		PP_1955	0.44	-0.1
2,212,457	+		PP_1955	0.48	-0.9
2,212,457	+		PP_1955	0.48	+0.4
2,212,457	+		PP_1955	0.48	-2.4
2,212,458	-		PP_1955	0.48	+0.1
2,212,458	-		PP_1955	0.48	-0.2
2,212,458	-		PP_1955	0.48	+0.3
2,212,475	+		PP_1955	0.49	-0.6
2,212,482	-		PP_1955	0.50	-1.3