Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1256 and PP_1257 are separated by 152 nucleotides PP_1257 and PP_1258 are separated by 42 nucleotides PP_1258 and PP_1259 are separated by 23 nucleotides
PP_1256: PP_1256 - putative alpha-ketoglutarate semialdehyde dehydrogenase, at 1,434,386 to 1,435,963
_1256
PP_1257: PP_1257 - putative 1-pyrroline-4-hydroxy-2-carboxylate deaminase, at 1,436,116 to 1,437,063
_1257
PP_1258: PP_1258 - 4-hydroxyproline epimerase, at 1,437,106 to 1,438,032
_1258
PP_1259: PP_1259 - Aspartate-proton symporter, at 1,438,056 to 1,439,669
_1259
Position (kb)
1436
1437
1438 Strain fitness (log2 ratio)
-1
0
1 at 1435.265 kb on - strand, within PP_1256 at 1435.265 kb on - strand, within PP_1256 at 1435.634 kb on + strand, within PP_1256 at 1435.634 kb on + strand, within PP_1256 at 1435.649 kb on - strand, within PP_1256 at 1435.649 kb on - strand, within PP_1256 at 1435.649 kb on - strand, within PP_1256 at 1435.791 kb on + strand, within PP_1256 at 1435.792 kb on - strand, within PP_1256 at 1435.857 kb on + strand at 1435.965 kb on + strand at 1435.965 kb on + strand at 1435.982 kb on + strand at 1435.982 kb on + strand at 1435.983 kb on - strand at 1436.006 kb on - strand at 1436.006 kb on - strand at 1436.006 kb on - strand at 1436.150 kb on + strand at 1436.150 kb on + strand at 1436.151 kb on - strand at 1436.172 kb on - strand at 1436.172 kb on - strand at 1436.186 kb on + strand at 1436.186 kb on + strand at 1436.187 kb on - strand at 1436.187 kb on - strand at 1436.237 kb on + strand, within PP_1257 at 1436.273 kb on + strand, within PP_1257 at 1436.274 kb on - strand, within PP_1257 at 1436.274 kb on - strand, within PP_1257 at 1436.274 kb on - strand, within PP_1257 at 1436.276 kb on + strand, within PP_1257 at 1436.277 kb on - strand, within PP_1257 at 1436.277 kb on - strand, within PP_1257 at 1436.277 kb on - strand, within PP_1257 at 1436.617 kb on + strand, within PP_1257 at 1436.645 kb on + strand, within PP_1257 at 1436.645 kb on + strand, within PP_1257 at 1436.645 kb on + strand, within PP_1257 at 1436.645 kb on + strand, within PP_1257 at 1436.657 kb on + strand, within PP_1257 at 1436.657 kb on + strand, within PP_1257 at 1436.657 kb on + strand, within PP_1257 at 1436.657 kb on + strand, within PP_1257 at 1436.657 kb on + strand, within PP_1257 at 1436.657 kb on + strand, within PP_1257 at 1436.658 kb on - strand, within PP_1257 at 1436.930 kb on + strand, within PP_1257 at 1436.931 kb on - strand, within PP_1257 at 1436.931 kb on - strand, within PP_1257 at 1437.168 kb on - strand at 1437.168 kb on - strand at 1437.168 kb on - strand at 1437.341 kb on + strand, within PP_1258 at 1437.342 kb on - strand, within PP_1258 at 1437.342 kb on - strand, within PP_1258 at 1437.342 kb on - strand, within PP_1258 at 1437.479 kb on + strand, within PP_1258 at 1437.480 kb on - strand, within PP_1258 at 1437.736 kb on + strand, within PP_1258 at 1437.737 kb on - strand, within PP_1258 at 1437.843 kb on - strand, within PP_1258 at 1437.843 kb on - strand, within PP_1258 at 1438.023 kb on + strand at 1438.049 kb on + strand at 1438.050 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1 remove 1,435,265 - PP_1256 0.56 +0.0 1,435,265 - PP_1256 0.56 -0.4 1,435,634 + PP_1256 0.79 +0.2 1,435,634 + PP_1256 0.79 +0.0 1,435,649 - PP_1256 0.80 -0.4 1,435,649 - PP_1256 0.80 -0.0 1,435,649 - PP_1256 0.80 -0.2 1,435,791 + PP_1256 0.89 -1.7 1,435,792 - PP_1256 0.89 +0.3 1,435,857 + -0.0 1,435,965 + -0.4 1,435,965 + -0.3 1,435,982 + -0.9 1,435,982 + -0.4 1,435,983 - +1.7 1,436,006 - -0.1 1,436,006 - -0.0 1,436,006 - +0.1 1,436,150 + +0.4 1,436,150 + -0.3 1,436,151 - +0.1 1,436,172 - -0.7 1,436,172 - -0.9 1,436,186 + +0.2 1,436,186 + +0.3 1,436,187 - -0.4 1,436,187 - -0.2 1,436,237 + PP_1257 0.13 -0.9 1,436,273 + PP_1257 0.17 +0.0 1,436,274 - PP_1257 0.17 +0.1 1,436,274 - PP_1257 0.17 -0.2 1,436,274 - PP_1257 0.17 -0.3 1,436,276 + PP_1257 0.17 -0.4 1,436,277 - PP_1257 0.17 +0.1 1,436,277 - PP_1257 0.17 +0.2 1,436,277 - PP_1257 0.17 -0.0 1,436,617 + PP_1257 0.53 -0.3 1,436,645 + PP_1257 0.56 -0.1 1,436,645 + PP_1257 0.56 -0.4 1,436,645 + PP_1257 0.56 +0.8 1,436,645 + PP_1257 0.56 -0.1 1,436,657 + PP_1257 0.57 -0.8 1,436,657 + PP_1257 0.57 +1.0 1,436,657 + PP_1257 0.57 -0.7 1,436,657 + PP_1257 0.57 -0.1 1,436,657 + PP_1257 0.57 +0.2 1,436,657 + PP_1257 0.57 +1.1 1,436,658 - PP_1257 0.57 +0.1 1,436,930 + PP_1257 0.86 -0.2 1,436,931 - PP_1257 0.86 -0.6 1,436,931 - PP_1257 0.86 -0.3 1,437,168 - -0.0 1,437,168 - +0.2 1,437,168 - +0.2 1,437,341 + PP_1258 0.25 +0.8 1,437,342 - PP_1258 0.25 +0.0 1,437,342 - PP_1258 0.25 -0.5 1,437,342 - PP_1258 0.25 -0.8 1,437,479 + PP_1258 0.40 -0.5 1,437,480 - PP_1258 0.40 +0.1 1,437,736 + PP_1258 0.68 -0.5 1,437,737 - PP_1258 0.68 -0.7 1,437,843 - PP_1258 0.80 -0.6 1,437,843 - PP_1258 0.80 +0.4 1,438,023 + +0.5 1,438,049 + +0.4 1,438,050 - -0.0
Or see this region's nucleotide sequence