Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0843 and PP_0844 are separated by 28 nucleotides PP_0844 and PP_0845 are separated by 8 nucleotides PP_0845 and PP_0846 are separated by 43 nucleotides PP_0846 and PP_0847 are separated by 3 nucleotides PP_0847 and PP_0848 are separated by 10 nucleotides PP_0848 and PP_0849 are separated by 88 nucleotides
PP_0843: PP_0843 - scaffold protein involved in iron-sulfur cluster assembly, at 979,812 to 980,198
_0843
PP_0844: PP_0844 - iron-sulfur cluster assembly protein, copper(I) binding, at 980,227 to 980,550
_0844
PP_0845: PP_0845 - DnaJ-like molecular chaperone specific for IscU, at 980,559 to 981,080
_0845
PP_0846: PP_0846 - DnaK-like molecular chaperone specific for IscU, at 981,124 to 982,986
_0846
PP_0847: PP_0847 - ferredoxin, at 982,990 to 983,331
_0847
PP_0848: PP_0848 - donor of Fe2+ and regulator of cysteine desulfurase activity, at 983,342 to 983,542
_0848
PP_0849: PP_0849 - Nucleoside diphosphate kinase, at 983,631 to 984,056
_0849
Position (kb) 981
982
983 Strain fitness (log2 ratio) -1
0
1 at 980.196 kb on + strand at 980.215 kb on + strand at 980.215 kb on + strand at 980.215 kb on + strand at 980.564 kb on + strand at 981.106 kb on + strand at 981.106 kb on + strand at 981.106 kb on + strand at 981.111 kb on + strand at 981.111 kb on + strand at 981.111 kb on + strand at 982.727 kb on + strand, within PP_0846 at 982.727 kb on + strand, within PP_0846 at 982.727 kb on + strand, within PP_0846 at 983.339 kb on + strand at 983.339 kb on + strand at 983.340 kb on - strand at 983.340 kb on - strand at 983.340 kb on - strand at 983.340 kb on - strand at 983.340 kb on - strand at 983.365 kb on - strand, within PP_0848 at 983.417 kb on + strand, within PP_0848 at 983.417 kb on + strand, within PP_0848 at 983.418 kb on - strand, within PP_0848 at 983.418 kb on - strand, within PP_0848 at 983.540 kb on + strand at 983.540 kb on + strand at 983.583 kb on + strand at 983.583 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1 remove 980,196 + +0.3 980,215 + +0.1 980,215 + -0.8 980,215 + -0.5 980,564 + +0.1 981,106 + -0.8 981,106 + +0.2 981,106 + -0.3 981,111 + +0.3 981,111 + -1.3 981,111 + +0.4 982,727 + PP_0846 0.86 -0.1 982,727 + PP_0846 0.86 +0.2 982,727 + PP_0846 0.86 +0.2 983,339 + +0.1 983,339 + -0.4 983,340 - -1.0 983,340 - -0.3 983,340 - +0.3 983,340 - -1.2 983,340 - -0.2 983,365 - PP_0848 0.11 -0.2 983,417 + PP_0848 0.37 +1.0 983,417 + PP_0848 0.37 -0.3 983,418 - PP_0848 0.38 -0.3 983,418 - PP_0848 0.38 +0.2 983,540 + -0.2 983,540 + -0.1 983,583 + -0.9 983,583 + -0.1
Or see this region's nucleotide sequence