Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 500 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5231 and PP_5232 are separated by 99 nucleotides PP_5232 and PP_5233 are separated by 429 nucleotides PP_5233 and PP_5234 are separated by 46 nucleotides PP_5234 and PP_5723 are separated by 134 nucleotides PP_5723 and PP_5235 are separated by 24 nucleotides
PP_5231: PP_5231 - putative (S)-2-haloacid dehalogenase, at 5,964,807 to 5,965,502
_5231
PP_5232: PP_5232 - conserved protein of unknown function, at 5,965,602 to 5,965,931
_5232
PP_5233: PP_5233 - ammonium transporter AmtB, at 5,966,361 to 5,967,692
_5233
PP_5234: PP_5234 - activator of NRII(GlnL/NtrB) phosphatase, at 5,967,739 to 5,968,077
_5234
PP_5723: PP_5723 - protein of unknown function, at 5,968,212 to 5,968,571
_5723
PP_5235: PP_5235 - conserved protein of unknown function, at 5,968,596 to 5,968,862
_5235
Position (kb)
5966
5967
5968 Strain fitness (log2 ratio)
-1
0
1
2
3 at 5965.558 kb on + strand at 5965.558 kb on + strand at 5965.558 kb on + strand at 5965.622 kb on - strand at 5965.622 kb on - strand at 5965.767 kb on + strand, within PP_5232 at 5965.767 kb on + strand, within PP_5232 at 5965.936 kb on + strand at 5965.936 kb on + strand at 5965.937 kb on - strand at 5965.937 kb on - strand at 5965.937 kb on - strand at 5965.968 kb on + strand at 5965.969 kb on - strand at 5965.969 kb on - strand at 5966.025 kb on + strand at 5966.026 kb on - strand at 5966.109 kb on - strand at 5966.130 kb on - strand at 5966.141 kb on + strand at 5966.142 kb on - strand at 5966.142 kb on - strand at 5966.198 kb on + strand at 5966.199 kb on - strand at 5966.228 kb on + strand at 5966.229 kb on - strand at 5966.229 kb on - strand at 5966.247 kb on - strand at 5966.260 kb on - strand at 5966.260 kb on - strand at 5966.280 kb on - strand at 5966.362 kb on + strand at 5966.362 kb on + strand at 5966.363 kb on - strand at 5966.363 kb on - strand at 5966.363 kb on - strand at 5966.470 kb on + strand at 5966.470 kb on + strand at 5966.470 kb on + strand at 5966.471 kb on - strand at 5966.471 kb on - strand at 5966.471 kb on - strand at 5966.583 kb on + strand, within PP_5233 at 5966.584 kb on - strand, within PP_5233 at 5966.689 kb on + strand, within PP_5233 at 5966.900 kb on + strand, within PP_5233 at 5967.033 kb on + strand, within PP_5233 at 5967.033 kb on + strand, within PP_5233 at 5967.034 kb on - strand, within PP_5233 at 5967.034 kb on - strand, within PP_5233 at 5967.034 kb on - strand at 5967.163 kb on + strand, within PP_5233 at 5967.163 kb on + strand, within PP_5233 at 5967.163 kb on + strand, within PP_5233 at 5967.163 kb on + strand, within PP_5233 at 5967.164 kb on - strand, within PP_5233 at 5967.164 kb on - strand, within PP_5233 at 5967.164 kb on - strand, within PP_5233 at 5967.192 kb on + strand, within PP_5233 at 5967.192 kb on + strand, within PP_5233 at 5967.192 kb on + strand, within PP_5233 at 5967.192 kb on + strand, within PP_5233 at 5967.192 kb on + strand, within PP_5233 at 5967.433 kb on + strand, within PP_5233 at 5967.523 kb on + strand, within PP_5233 at 5967.523 kb on + strand, within PP_5233 at 5967.524 kb on - strand, within PP_5233 at 5967.524 kb on - strand, within PP_5233 at 5967.545 kb on + strand, within PP_5233 at 5967.546 kb on - strand, within PP_5233 at 5967.602 kb on + strand at 5967.603 kb on - strand at 5967.645 kb on + strand at 5967.645 kb on + strand at 5967.657 kb on + strand at 5967.658 kb on - strand at 5967.666 kb on + strand at 5967.667 kb on - strand at 5967.667 kb on - strand at 5967.740 kb on + strand at 5967.740 kb on + strand at 5967.740 kb on + strand at 5967.740 kb on + strand at 5967.740 kb on + strand at 5967.741 kb on - strand at 5967.741 kb on - strand at 5967.741 kb on - strand at 5968.135 kb on - strand at 5968.228 kb on - strand at 5968.283 kb on + strand, within PP_5723 at 5968.283 kb on + strand, within PP_5723 at 5968.284 kb on - strand at 5968.284 kb on - strand, within PP_5723 at 5968.470 kb on + strand, within PP_5723 at 5968.471 kb on - strand, within PP_5723 at 5968.471 kb on - strand, within PP_5723 at 5968.557 kb on + strand at 5968.557 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 500 uM; timepoint 1 remove 5,965,558 + +0.1 5,965,558 + +0.4 5,965,558 + -0.5 5,965,622 - +0.1 5,965,622 - +0.6 5,965,767 + PP_5232 0.50 +0.4 5,965,767 + PP_5232 0.50 -0.3 5,965,936 + +0.1 5,965,936 + +0.5 5,965,937 - +0.5 5,965,937 - -0.1 5,965,937 - +0.3 5,965,968 + +0.4 5,965,969 - -0.6 5,965,969 - -0.3 5,966,025 + -0.5 5,966,026 - -0.8 5,966,109 - -0.2 5,966,130 - +1.4 5,966,141 + +0.1 5,966,142 - +0.1 5,966,142 - -1.2 5,966,198 + +0.1 5,966,199 - +1.6 5,966,228 + +0.2 5,966,229 - +0.6 5,966,229 - -0.2 5,966,247 - -0.2 5,966,260 - +0.9 5,966,260 - +0.2 5,966,280 - +0.5 5,966,362 + +0.4 5,966,362 + +0.3 5,966,363 - +0.1 5,966,363 - -0.2 5,966,363 - -0.1 5,966,470 + +0.4 5,966,470 + +0.2 5,966,470 + -0.1 5,966,471 - +1.7 5,966,471 - +0.3 5,966,471 - -0.5 5,966,583 + PP_5233 0.17 +0.2 5,966,584 - PP_5233 0.17 -0.9 5,966,689 + PP_5233 0.25 +0.1 5,966,900 + PP_5233 0.40 +0.5 5,967,033 + PP_5233 0.50 +0.5 5,967,033 + PP_5233 0.50 +0.0 5,967,034 - PP_5233 0.51 -0.0 5,967,034 - PP_5233 0.51 +1.4 5,967,034 - +2.6 5,967,163 + PP_5233 0.60 +1.0 5,967,163 + PP_5233 0.60 +1.0 5,967,163 + PP_5233 0.60 +0.0 5,967,163 + PP_5233 0.60 +0.2 5,967,164 - PP_5233 0.60 -0.1 5,967,164 - PP_5233 0.60 -0.2 5,967,164 - PP_5233 0.60 -0.3 5,967,192 + PP_5233 0.62 -0.2 5,967,192 + PP_5233 0.62 -0.7 5,967,192 + PP_5233 0.62 +0.8 5,967,192 + PP_5233 0.62 -0.3 5,967,192 + PP_5233 0.62 -0.1 5,967,433 + PP_5233 0.80 -0.2 5,967,523 + PP_5233 0.87 -0.2 5,967,523 + PP_5233 0.87 +0.5 5,967,524 - PP_5233 0.87 +0.1 5,967,524 - PP_5233 0.87 -0.1 5,967,545 + PP_5233 0.89 -1.4 5,967,546 - PP_5233 0.89 -0.6 5,967,602 + +0.3 5,967,603 - -0.3 5,967,645 + -0.3 5,967,645 + -0.4 5,967,657 + -0.1 5,967,658 - -0.2 5,967,666 + -0.6 5,967,667 - +0.1 5,967,667 - -0.2 5,967,740 + -0.4 5,967,740 + -0.3 5,967,740 + -0.7 5,967,740 + -0.2 5,967,740 + -0.0 5,967,741 - +1.1 5,967,741 - -0.6 5,967,741 - -0.6 5,968,135 - -0.1 5,968,228 - +0.1 5,968,283 + PP_5723 0.20 +0.3 5,968,283 + PP_5723 0.20 -0.6 5,968,284 - +3.6 5,968,284 - PP_5723 0.20 -0.2 5,968,470 + PP_5723 0.72 -0.2 5,968,471 - PP_5723 0.72 -0.2 5,968,471 - PP_5723 0.72 -0.4 5,968,557 + +0.0 5,968,557 + -0.2
Or see this region's nucleotide sequence