Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 500 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1960 and PP_1961 are separated by 3 nucleotides PP_1961 and PP_1962 overlap by 1 nucleotides
PP_1960: PP_1960 - conserved protein of unknown function, at 2,220,303 to 2,221,625
_1960
PP_1961: PP_1961 - conserved protein of unknown function, at 2,221,629 to 2,222,618
_1961
PP_1962: PP_1962 - Site-specific recombinase, phage integrase family, at 2,222,618 to 2,224,546
_1962
Position (kb)
2221
2222
2223 Strain fitness (log2 ratio)
-2
-1
0
1 at 2220.798 kb on - strand, within PP_1960 at 2220.798 kb on - strand, within PP_1960 at 2220.811 kb on - strand, within PP_1960 at 2220.854 kb on + strand, within PP_1960 at 2220.858 kb on - strand, within PP_1960 at 2220.871 kb on + strand, within PP_1960 at 2220.926 kb on + strand, within PP_1960 at 2220.958 kb on + strand, within PP_1960 at 2221.069 kb on - strand, within PP_1960 at 2221.159 kb on + strand, within PP_1960 at 2221.167 kb on + strand, within PP_1960 at 2221.273 kb on - strand, within PP_1960 at 2221.384 kb on + strand, within PP_1960 at 2221.400 kb on - strand, within PP_1960 at 2221.437 kb on + strand, within PP_1960 at 2221.446 kb on + strand, within PP_1960 at 2221.447 kb on - strand, within PP_1960 at 2221.447 kb on - strand, within PP_1960 at 2221.562 kb on - strand at 2221.660 kb on - strand at 2221.674 kb on + strand at 2221.783 kb on + strand, within PP_1961 at 2221.783 kb on + strand, within PP_1961 at 2221.967 kb on + strand, within PP_1961 at 2222.094 kb on + strand, within PP_1961 at 2222.173 kb on - strand, within PP_1961 at 2222.177 kb on + strand, within PP_1961 at 2222.272 kb on - strand, within PP_1961 at 2222.299 kb on + strand, within PP_1961 at 2222.299 kb on + strand, within PP_1961 at 2222.299 kb on + strand, within PP_1961 at 2222.299 kb on + strand, within PP_1961 at 2222.299 kb on + strand, within PP_1961 at 2222.300 kb on - strand, within PP_1961 at 2222.396 kb on + strand, within PP_1961 at 2222.397 kb on - strand, within PP_1961 at 2222.397 kb on - strand, within PP_1961 at 2222.445 kb on - strand, within PP_1961 at 2222.482 kb on + strand, within PP_1961 at 2222.483 kb on - strand, within PP_1961 at 2222.541 kb on + strand at 2222.549 kb on + strand at 2222.625 kb on - strand at 2222.772 kb on + strand at 2222.816 kb on + strand, within PP_1962 at 2222.827 kb on + strand, within PP_1962 at 2222.943 kb on + strand, within PP_1962 at 2222.943 kb on + strand, within PP_1962 at 2222.943 kb on + strand, within PP_1962 at 2222.943 kb on + strand, within PP_1962 at 2222.943 kb on + strand, within PP_1962 at 2222.943 kb on + strand, within PP_1962 at 2222.943 kb on + strand, within PP_1962 at 2222.970 kb on + strand, within PP_1962 at 2222.970 kb on + strand, within PP_1962 at 2223.008 kb on + strand, within PP_1962 at 2223.008 kb on + strand, within PP_1962 at 2223.020 kb on + strand, within PP_1962 at 2223.166 kb on + strand, within PP_1962 at 2223.166 kb on + strand, within PP_1962 at 2223.170 kb on + strand, within PP_1962 at 2223.170 kb on + strand, within PP_1962 at 2223.222 kb on + strand, within PP_1962 at 2223.222 kb on + strand, within PP_1962 at 2223.438 kb on + strand, within PP_1962 at 2223.451 kb on + strand, within PP_1962
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 500 uM; timepoint 1 remove 2,220,798 - PP_1960 0.37 +0.3 2,220,798 - PP_1960 0.37 -0.2 2,220,811 - PP_1960 0.38 -0.6 2,220,854 + PP_1960 0.42 -0.1 2,220,858 - PP_1960 0.42 -1.4 2,220,871 + PP_1960 0.43 +0.3 2,220,926 + PP_1960 0.47 -0.2 2,220,958 + PP_1960 0.50 -1.1 2,221,069 - PP_1960 0.58 -0.4 2,221,159 + PP_1960 0.65 -0.2 2,221,167 + PP_1960 0.65 -0.5 2,221,273 - PP_1960 0.73 -1.0 2,221,384 + PP_1960 0.82 +0.5 2,221,400 - PP_1960 0.83 +1.3 2,221,437 + PP_1960 0.86 -0.3 2,221,446 + PP_1960 0.86 +0.1 2,221,447 - PP_1960 0.86 -0.7 2,221,447 - PP_1960 0.86 -0.9 2,221,562 - +0.2 2,221,660 - -0.1 2,221,674 + +0.4 2,221,783 + PP_1961 0.16 +0.7 2,221,783 + PP_1961 0.16 -0.2 2,221,967 + PP_1961 0.34 -1.1 2,222,094 + PP_1961 0.47 -0.3 2,222,173 - PP_1961 0.55 -0.1 2,222,177 + PP_1961 0.55 -0.2 2,222,272 - PP_1961 0.65 -1.9 2,222,299 + PP_1961 0.68 +0.1 2,222,299 + PP_1961 0.68 -0.5 2,222,299 + PP_1961 0.68 -0.5 2,222,299 + PP_1961 0.68 -0.5 2,222,299 + PP_1961 0.68 -1.5 2,222,300 - PP_1961 0.68 +0.2 2,222,396 + PP_1961 0.77 -0.8 2,222,397 - PP_1961 0.78 -0.1 2,222,397 - PP_1961 0.78 -0.5 2,222,445 - PP_1961 0.82 -0.4 2,222,482 + PP_1961 0.86 -0.2 2,222,483 - PP_1961 0.86 -1.5 2,222,541 + +0.6 2,222,549 + -0.6 2,222,625 - -0.1 2,222,772 + +0.7 2,222,816 + PP_1962 0.10 +0.5 2,222,827 + PP_1962 0.11 -0.9 2,222,943 + PP_1962 0.17 -0.7 2,222,943 + PP_1962 0.17 -0.4 2,222,943 + PP_1962 0.17 -0.6 2,222,943 + PP_1962 0.17 +0.2 2,222,943 + PP_1962 0.17 -0.7 2,222,943 + PP_1962 0.17 -0.6 2,222,943 + PP_1962 0.17 -0.3 2,222,970 + PP_1962 0.18 -0.3 2,222,970 + PP_1962 0.18 +1.4 2,223,008 + PP_1962 0.20 -0.4 2,223,008 + PP_1962 0.20 -0.1 2,223,020 + PP_1962 0.21 +0.7 2,223,166 + PP_1962 0.28 -0.3 2,223,166 + PP_1962 0.28 -1.2 2,223,170 + PP_1962 0.29 -0.8 2,223,170 + PP_1962 0.29 -0.9 2,223,222 + PP_1962 0.31 -0.6 2,223,222 + PP_1962 0.31 -0.0 2,223,438 + PP_1962 0.43 +0.5 2,223,451 + PP_1962 0.43 -0.8
Or see this region's nucleotide sequence