Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4760 and PP_4761 are separated by 164 nucleotides PP_4761 and PP_4762 overlap by 4 nucleotides PP_4762 and PP_4763 are separated by 149 nucleotides PP_4763 and PP_5712 are separated by 213 nucleotides
PP_4760: PP_4760 - Alcohol dehydrogenase, zinc-containing, at 5,420,088 to 5,421,098
_4760
PP_4761: PP_4761 - Hydrolase, haloacid dehalogenase-like family, at 5,421,263 to 5,421,853
_4761
PP_4762: PP_4762 - Acyl-CoA thioesterase II, at 5,421,850 to 5,422,719
_4762
PP_4763: PP_4763 - GCN5-related N-acetyltransferase, at 5,422,869 to 5,423,405
_4763
PP_5712: PP_5712 - conserved protein of unknown function, at 5,423,619 to 5,424,305
_5712
Position (kb)
5421
5422
5423 Strain fitness (log2 ratio)
-2
-1
0
1 at 5420.971 kb on + strand, within PP_4760 at 5420.972 kb on - strand, within PP_4760 at 5421.075 kb on + strand at 5421.075 kb on + strand at 5421.076 kb on - strand at 5421.076 kb on - strand at 5421.079 kb on + strand at 5421.079 kb on + strand at 5421.079 kb on + strand at 5421.079 kb on + strand at 5421.080 kb on - strand at 5421.080 kb on - strand at 5421.140 kb on - strand at 5421.211 kb on - strand at 5421.584 kb on - strand, within PP_4761 at 5421.980 kb on + strand, within PP_4762 at 5421.981 kb on - strand, within PP_4762 at 5421.981 kb on - strand, within PP_4762 at 5422.119 kb on - strand, within PP_4762 at 5422.130 kb on + strand, within PP_4762 at 5422.131 kb on - strand, within PP_4762 at 5422.184 kb on + strand, within PP_4762 at 5422.185 kb on - strand, within PP_4762 at 5422.185 kb on - strand, within PP_4762 at 5422.391 kb on + strand, within PP_4762 at 5422.392 kb on - strand, within PP_4762 at 5422.455 kb on + strand, within PP_4762 at 5422.532 kb on + strand, within PP_4762 at 5422.617 kb on - strand, within PP_4762 at 5422.617 kb on - strand, within PP_4762 at 5422.617 kb on - strand, within PP_4762 at 5422.644 kb on + strand at 5422.645 kb on - strand at 5422.645 kb on - strand at 5422.733 kb on + strand at 5422.734 kb on - strand at 5422.745 kb on + strand at 5422.746 kb on - strand at 5422.800 kb on - strand at 5422.820 kb on + strand at 5422.820 kb on + strand at 5422.831 kb on + strand at 5423.477 kb on + strand at 5423.478 kb on - strand at 5423.600 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 remove 5,420,971 + PP_4760 0.87 -0.5 5,420,972 - PP_4760 0.87 +0.7 5,421,075 + +0.2 5,421,075 + -0.3 5,421,076 - -0.7 5,421,076 - +0.4 5,421,079 + -0.2 5,421,079 + -0.2 5,421,079 + +0.0 5,421,079 + +0.5 5,421,080 - +0.5 5,421,080 - +0.2 5,421,140 - +0.3 5,421,211 - -0.3 5,421,584 - PP_4761 0.54 -2.5 5,421,980 + PP_4762 0.15 +0.1 5,421,981 - PP_4762 0.15 -0.4 5,421,981 - PP_4762 0.15 -0.3 5,422,119 - PP_4762 0.31 +0.1 5,422,130 + PP_4762 0.32 -0.1 5,422,131 - PP_4762 0.32 +0.9 5,422,184 + PP_4762 0.38 -0.1 5,422,185 - PP_4762 0.39 -0.2 5,422,185 - PP_4762 0.39 -0.4 5,422,391 + PP_4762 0.62 -0.1 5,422,392 - PP_4762 0.62 +0.4 5,422,455 + PP_4762 0.70 +0.2 5,422,532 + PP_4762 0.78 +0.2 5,422,617 - PP_4762 0.88 +0.1 5,422,617 - PP_4762 0.88 -0.2 5,422,617 - PP_4762 0.88 -0.2 5,422,644 + -0.8 5,422,645 - -0.5 5,422,645 - -1.3 5,422,733 + -1.7 5,422,734 - -0.6 5,422,745 + -0.3 5,422,746 - -0.5 5,422,800 - -0.1 5,422,820 + -0.8 5,422,820 + -0.6 5,422,831 + -0.7 5,423,477 + -1.8 5,423,478 - -0.2 5,423,600 - -0.4
Or see this region's nucleotide sequence