Strain Fitness in Pseudomonas putida KT2440 around PP_3754

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
4,282,440	+		PP_3753	0.27	+0.9
4,282,520	+		PP_3753	0.35	+0.2
4,282,521	-		PP_3753	0.35	+0.0
4,282,634	+		PP_3753	0.45	+1.2
4,282,634	+		PP_3753	0.45	-0.0
4,282,802	+		PP_3753	0.61	-0.3
4,282,803	-		PP_3753	0.61	+0.0
4,282,803	-		PP_3753	0.61	+0.1
4,282,803	-		PP_3753	0.61	-0.8
4,282,817	+		PP_3753	0.62	+0.2
4,282,818	-		PP_3753	0.62	-0.4
4,282,880	-				-0.0
4,283,201	+				-0.0
4,283,201	+				-0.2
4,283,202	-				-0.2
4,283,247	-				+0.3
4,283,247	-				+0.2
4,283,247	-				+0.3
4,283,292	+				-0.2
4,283,293	-				+0.8
4,283,397	+				-0.9
4,283,397	+				-0.6
4,283,398	-				+0.4
4,283,398	-				-0.3
4,283,416	+				+0.4
4,283,571	-		PP_3754	0.15	-0.2
4,283,689	-		PP_3754	0.25	-0.0
4,283,689	-		PP_3754	0.25	-0.7
4,283,774	-		PP_3754	0.32	-0.3
4,283,774	-		PP_3754	0.32	+0.3
4,283,831	-		PP_3754	0.37	-0.1
4,283,832	+		PP_3754	0.37	+0.4
4,283,832	+		PP_3754	0.37	+0.5
4,283,833	-		PP_3754	0.37	-0.2
4,284,236	-				-1.0
4,284,537	+				-0.6
4,284,824	+		PP_3755	0.27	+0.5
4,284,825	-		PP_3755	0.27	+0.8
4,284,946	+		PP_3755	0.41	-0.0
4,284,946	+		PP_3755	0.41	-0.4
4,284,946	+		PP_3755	0.41	-0.1
4,284,946	+		PP_3755	0.41	-0.4
4,284,947	-		PP_3755	0.41	+0.4
4,284,947	-		PP_3755	0.41	-0.0
4,284,947	-		PP_3755	0.41	-0.2
4,285,086	-		PP_3755	0.58	-0.1
4,285,362	-				+0.2
4,285,440	-				-0.1
4,285,440	-				+0.3