Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2679 and PP_2680 are separated by 162 nucleotides PP_2680 and PP_2681 are separated by 353 nucleotides PP_2681 and PP_2682 are separated by 89 nucleotides
PP_2679: PP_2679 - quinoprotein ethanol dehydrogenase, at 3,066,962 to 3,068,749
_2679
PP_2680: PP_2680 - aldehyde dehydrogenase, at 3,068,912 to 3,070,432
_2680
PP_2681: PP_2681 - coenzyme PQQ synthesis protein D, at 3,070,786 to 3,071,058
_2681
PP_2682: PP_2682 - Fe-containing alcohol dehydrogenase, at 3,071,148 to 3,072,311
_2682
Position (kb)
3068
3069
3070
3071 Strain fitness (log2 ratio)
-1
0
1 at 3068.024 kb on + strand, within PP_2679 at 3068.228 kb on + strand, within PP_2679 at 3068.229 kb on - strand, within PP_2679 at 3068.229 kb on - strand, within PP_2679 at 3068.229 kb on - strand, within PP_2679 at 3068.229 kb on - strand, within PP_2679 at 3068.252 kb on + strand, within PP_2679 at 3068.252 kb on + strand, within PP_2679 at 3068.252 kb on + strand, within PP_2679 at 3068.303 kb on + strand, within PP_2679 at 3068.304 kb on - strand, within PP_2679 at 3068.304 kb on - strand, within PP_2679 at 3068.304 kb on - strand, within PP_2679 at 3068.305 kb on + strand, within PP_2679 at 3068.306 kb on - strand, within PP_2679 at 3068.363 kb on + strand, within PP_2679 at 3068.426 kb on + strand, within PP_2679 at 3068.427 kb on - strand, within PP_2679 at 3068.427 kb on - strand, within PP_2679 at 3068.600 kb on + strand at 3068.601 kb on - strand at 3068.601 kb on - strand at 3068.601 kb on - strand at 3068.610 kb on + strand at 3068.748 kb on - strand at 3068.759 kb on - strand at 3068.759 kb on - strand at 3068.797 kb on + strand at 3068.797 kb on + strand at 3068.801 kb on - strand at 3068.801 kb on - strand at 3068.858 kb on + strand at 3068.858 kb on + strand at 3068.918 kb on + strand at 3068.919 kb on - strand at 3068.919 kb on - strand at 3069.014 kb on + strand at 3069.328 kb on - strand, within PP_2680 at 3069.851 kb on + strand, within PP_2680 at 3069.852 kb on - strand, within PP_2680 at 3069.956 kb on + strand, within PP_2680 at 3070.062 kb on - strand, within PP_2680 at 3070.062 kb on - strand, within PP_2680 at 3070.062 kb on - strand, within PP_2680 at 3070.062 kb on - strand, within PP_2680 at 3070.286 kb on + strand at 3070.287 kb on - strand at 3070.287 kb on - strand at 3070.322 kb on + strand at 3070.374 kb on - strand at 3070.403 kb on + strand at 3070.404 kb on - strand at 3070.557 kb on - strand at 3070.588 kb on - strand at 3070.671 kb on + strand at 3070.681 kb on + strand at 3070.681 kb on + strand at 3070.683 kb on + strand at 3070.717 kb on + strand at 3070.718 kb on - strand at 3070.777 kb on + strand at 3070.778 kb on - strand at 3070.876 kb on + strand, within PP_2681 at 3070.877 kb on - strand, within PP_2681 at 3071.051 kb on + strand at 3071.052 kb on - strand at 3071.104 kb on + strand at 3071.104 kb on + strand at 3071.105 kb on - strand at 3071.105 kb on - strand at 3071.131 kb on + strand at 3071.131 kb on + strand at 3071.132 kb on - strand at 3071.143 kb on + strand at 3071.261 kb on - strand at 3071.343 kb on + strand, within PP_2682 at 3071.344 kb on - strand, within PP_2682 at 3071.353 kb on - strand, within PP_2682 at 3071.409 kb on + strand, within PP_2682 at 3071.410 kb on - strand, within PP_2682
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 remove 3,068,024 + PP_2679 0.59 -0.4 3,068,228 + PP_2679 0.71 -0.9 3,068,229 - PP_2679 0.71 +0.5 3,068,229 - PP_2679 0.71 +0.2 3,068,229 - PP_2679 0.71 +0.2 3,068,229 - PP_2679 0.71 -0.1 3,068,252 + PP_2679 0.72 -0.3 3,068,252 + PP_2679 0.72 -0.1 3,068,252 + PP_2679 0.72 +0.4 3,068,303 + PP_2679 0.75 +0.2 3,068,304 - PP_2679 0.75 -0.5 3,068,304 - PP_2679 0.75 -0.1 3,068,304 - PP_2679 0.75 +0.4 3,068,305 + PP_2679 0.75 -0.1 3,068,306 - PP_2679 0.75 +0.1 3,068,363 + PP_2679 0.78 -1.0 3,068,426 + PP_2679 0.82 +0.3 3,068,427 - PP_2679 0.82 -0.6 3,068,427 - PP_2679 0.82 +0.1 3,068,600 + -0.1 3,068,601 - +0.7 3,068,601 - -0.2 3,068,601 - -0.5 3,068,610 + +0.3 3,068,748 - -0.1 3,068,759 - +0.5 3,068,759 - -0.1 3,068,797 + -0.9 3,068,797 + +0.0 3,068,801 - +1.4 3,068,801 - +1.0 3,068,858 + -0.6 3,068,858 + +0.3 3,068,918 + +1.1 3,068,919 - -1.2 3,068,919 - -0.1 3,069,014 + -0.2 3,069,328 - PP_2680 0.27 +0.0 3,069,851 + PP_2680 0.62 +0.2 3,069,852 - PP_2680 0.62 +0.2 3,069,956 + PP_2680 0.69 +0.9 3,070,062 - PP_2680 0.76 -0.5 3,070,062 - PP_2680 0.76 -0.7 3,070,062 - PP_2680 0.76 +0.0 3,070,062 - PP_2680 0.76 -0.2 3,070,286 + +0.0 3,070,287 - -0.2 3,070,287 - -0.9 3,070,322 + -0.5 3,070,374 - -0.5 3,070,403 + -0.2 3,070,404 - +0.3 3,070,557 - -0.5 3,070,588 - -0.1 3,070,671 + -0.1 3,070,681 + +0.3 3,070,681 + +0.2 3,070,683 + -0.0 3,070,717 + +0.7 3,070,718 - -0.1 3,070,777 + +0.4 3,070,778 - +0.1 3,070,876 + PP_2681 0.33 -0.1 3,070,877 - PP_2681 0.33 +0.8 3,071,051 + +0.2 3,071,052 - +0.6 3,071,104 + +0.2 3,071,104 + -0.1 3,071,105 - -0.1 3,071,105 - -0.1 3,071,131 + +0.2 3,071,131 + -0.3 3,071,132 - +0.2 3,071,143 + -0.4 3,071,261 - -0.3 3,071,343 + PP_2682 0.17 +0.3 3,071,344 - PP_2682 0.17 +0.8 3,071,353 - PP_2682 0.18 -0.5 3,071,409 + PP_2682 0.22 +0.2 3,071,410 - PP_2682 0.23 -0.5
Or see this region's nucleotide sequence