Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0510 and PP_0511 are separated by 31 nucleotides PP_0511 and PP_0512 are separated by 0 nucleotides PP_0512 and PP_0513 are separated by 130 nucleotides PP_0513 and PP_0514 overlap by 4 nucleotides
PP_0510: PP_0510 - Thioredoxin, at 598,072 to 598,944
_0510
PP_0511: PP_0511 - putative methyltransferase, at 598,976 to 599,635
_0511
PP_0512: PP_0512 - hypothetical protein, at 599,636 to 600,043
_0512
PP_0513: PP_0513 - DNA-binding transcriptional repressor NrdR-Zn2+-ATP/dATP, at 600,174 to 600,653
_0513
PP_0514: PP_0514 - bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase, at 600,650 to 601,786
_0514
Position (kb) 599
600
601 Strain fitness (log2 ratio) -1
0
1 at 598.926 kb on - strand at 598.926 kb on - strand at 598.966 kb on + strand at 598.966 kb on + strand at 598.967 kb on - strand at 598.977 kb on + strand at 598.978 kb on - strand at 598.978 kb on - strand at 598.979 kb on + strand at 598.979 kb on + strand at 598.979 kb on + strand at 598.980 kb on - strand at 598.980 kb on - strand at 598.980 kb on - strand at 598.980 kb on - strand at 599.001 kb on + strand at 599.079 kb on + strand, within PP_0511 at 599.080 kb on - strand, within PP_0511 at 599.080 kb on - strand, within PP_0511 at 599.092 kb on + strand, within PP_0511 at 599.179 kb on - strand, within PP_0511 at 599.179 kb on - strand, within PP_0511 at 599.241 kb on + strand, within PP_0511 at 599.242 kb on - strand, within PP_0511 at 599.242 kb on - strand, within PP_0511 at 599.242 kb on - strand, within PP_0511 at 599.246 kb on + strand, within PP_0511 at 599.246 kb on + strand, within PP_0511 at 599.246 kb on + strand, within PP_0511 at 599.247 kb on - strand, within PP_0511 at 599.425 kb on - strand, within PP_0511 at 599.466 kb on + strand, within PP_0511 at 599.543 kb on + strand, within PP_0511 at 599.544 kb on - strand, within PP_0511 at 599.637 kb on + strand at 599.637 kb on + strand at 599.637 kb on + strand at 599.638 kb on - strand at 599.638 kb on - strand at 599.638 kb on - strand at 599.800 kb on - strand, within PP_0512 at 599.800 kb on - strand, within PP_0512 at 599.943 kb on + strand, within PP_0512 at 600.075 kb on + strand at 600.076 kb on - strand at 600.103 kb on + strand at 600.103 kb on + strand at 600.104 kb on - strand at 600.104 kb on - strand at 600.558 kb on + strand at 600.558 kb on + strand, within PP_0513 at 600.558 kb on + strand, within PP_0513 at 600.558 kb on + strand, within PP_0513 at 600.579 kb on + strand, within PP_0513 at 600.689 kb on + strand at 600.689 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 remove 598,926 - -0.3 598,926 - +0.3 598,966 + -0.1 598,966 + -0.1 598,967 - +0.1 598,977 + -0.4 598,978 - +0.2 598,978 - -1.7 598,979 + +0.5 598,979 + -0.1 598,979 + +1.3 598,980 - +0.1 598,980 - +0.3 598,980 - -0.5 598,980 - +0.4 599,001 + +0.6 599,079 + PP_0511 0.16 +0.4 599,080 - PP_0511 0.16 +0.2 599,080 - PP_0511 0.16 +0.6 599,092 + PP_0511 0.18 +0.3 599,179 - PP_0511 0.31 +0.3 599,179 - PP_0511 0.31 -0.0 599,241 + PP_0511 0.40 +0.8 599,242 - PP_0511 0.40 -0.3 599,242 - PP_0511 0.40 -0.2 599,242 - PP_0511 0.40 +0.3 599,246 + PP_0511 0.41 -0.2 599,246 + PP_0511 0.41 +0.5 599,246 + PP_0511 0.41 -0.5 599,247 - PP_0511 0.41 -0.3 599,425 - PP_0511 0.68 +0.2 599,466 + PP_0511 0.74 -0.4 599,543 + PP_0511 0.86 -0.3 599,544 - PP_0511 0.86 +0.3 599,637 + -0.2 599,637 + +0.2 599,637 + +0.3 599,638 - -0.2 599,638 - -0.5 599,638 - +0.5 599,800 - PP_0512 0.40 -0.3 599,800 - PP_0512 0.40 -0.1 599,943 + PP_0512 0.75 -1.2 600,075 + +0.3 600,076 - -0.2 600,103 + +0.2 600,103 + -0.3 600,104 - +0.3 600,104 - -0.2 600,558 + +1.6 600,558 + PP_0513 0.80 -1.2 600,558 + PP_0513 0.80 -0.4 600,558 + PP_0513 0.80 +0.9 600,579 + PP_0513 0.84 +0.1 600,689 + +1.2 600,689 + -0.0
Or see this region's nucleotide sequence