Strain Fitness in Pseudomonas putida KT2440 around PP_4031

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
4,540,418	+		PP_4030	0.12	+0.4
4,540,418	+		PP_4030	0.12	-0.0
4,540,419	-		PP_4030	0.12	+0.0
4,540,454	+		PP_4030	0.16	+0.2
4,540,455	-		PP_4030	0.16	-0.4
4,540,455	-		PP_4030	0.16	-0.4
4,540,556	+		PP_4030	0.28	+0.8
4,540,556	+		PP_4030	0.28	-0.7
4,540,557	-		PP_4030	0.28	+0.4
4,540,557	-		PP_4030	0.28	+0.1
4,540,557	-		PP_4030	0.28	+0.4
4,540,614	+		PP_4030	0.35	+0.5
4,540,785	-		PP_4030	0.55	+0.9
4,541,123	+				-0.0
4,541,124	-				+0.5
4,541,194	+				+0.0
4,541,194	+				+0.3
4,541,194	+				-0.7
4,541,194	+				+0.5
4,541,194	+				+0.4
4,541,194	+				+0.5
4,541,194	+				+0.3
4,541,194	+				-0.1
4,541,195	-				-0.3
4,541,195	-				+0.1
4,541,195	-				+0.9
4,541,195	-				+0.9
4,541,195	-				+0.0
4,541,195	-				+0.1
4,541,195	-				-0.3
4,541,237	+				-0.0
4,541,237	+				+0.2
4,541,237	+				-1.3
4,541,237	+				+1.7
4,541,237	+				+0.5
4,541,238	-				+0.3
4,541,238	-				-0.2
4,541,238	-				-0.7
4,541,238	-				+0.0
4,541,238	-				+0.2
4,541,238	-				+0.7
4,541,240	-				+0.1
4,541,267	+				+0.1
4,541,269	+				+0.3
4,541,269	+				+1.5
4,541,269	+				+0.2
4,541,270	-				+0.3
4,541,270	-				+0.0
4,541,275	+				-0.2
4,541,275	+				+0.2
4,541,275	+				-0.2
4,541,275	+				+0.1
4,541,275	+				-0.6
4,541,275	+				-0.4
4,541,275	+				+0.1
4,541,276	-				-0.1
4,541,276	-				+0.2
4,541,276	-				+0.2
4,541,276	-				+0.0
4,541,279	+				+0.0
4,541,279	+				+0.4
4,541,279	+				+0.2
4,541,279	+				+0.8
4,541,279	+				+0.4
4,541,279	+				-0.1
4,541,279	+				+0.5
4,541,279	+				-0.2
4,541,279	+				+0.1
4,541,279	+				-0.2
4,541,279	+				-0.3
4,541,280	-				+0.3
4,541,280	-				+0.4
4,541,280	-				-0.6
4,541,280	-				-0.3
4,541,280	-				-0.3
4,541,280	-				+0.3
4,541,280	-				-0.2
4,541,280	-				-0.2
4,541,280	-				-0.0
4,541,280	-				-0.1
4,541,280	-				-0.1
4,541,280	-				-0.2
4,541,280	-				+0.0
4,541,280	-				-0.1
4,541,283	+				-0.5
4,541,284	-				+0.5
4,541,284	-				-0.4
4,541,284	-				+0.3
4,541,284	-				-0.1
4,541,284	-				+0.1
4,541,284	-				+0.3
4,541,344	+				+0.6
4,541,542	+		PP_4031	0.17	+0.3
4,541,542	+		PP_4031	0.17	-0.2
4,541,543	-		PP_4031	0.17	-0.3
4,541,543	-		PP_4031	0.17	-0.0
4,541,579	+		PP_4031	0.20	-0.4
4,541,771	-		PP_4031	0.32	-0.1
4,541,771	-		PP_4031	0.32	-0.2
4,541,779	+		PP_4031	0.33	-0.8
4,541,779	+		PP_4031	0.33	-0.6
4,541,780	-		PP_4031	0.33	-0.0
4,541,780	-		PP_4031	0.33	-0.6
4,541,780	-		PP_4031	0.33	-0.4
4,541,893	-		PP_4031	0.41	-0.1
4,542,194	+		PP_4031	0.61	-0.6
4,542,195	-		PP_4031	0.61	+0.8
4,542,195	-		PP_4031	0.61	-0.1
4,542,195	-		PP_4031	0.61	+0.2
4,542,278	+		PP_4031	0.66	-0.1
4,542,278	+		PP_4031	0.66	-0.1
4,542,279	-		PP_4031	0.66	-0.4
4,542,469	+		PP_4031	0.79	+0.2
4,542,470	-		PP_4031	0.79	-0.0
4,542,526	+		PP_4031	0.83	+0.2
4,542,526	+		PP_4031	0.83	-0.0
4,542,526	+		PP_4031	0.83	-0.6
4,542,526	+		PP_4031	0.83	-0.4
4,542,526	+		PP_4031	0.83	+0.7
4,542,526	+		PP_4031	0.83	-0.4
4,542,526	+		PP_4031	0.83	-0.2
4,542,526	+		PP_4031	0.83	-0.1
4,542,526	+		PP_4031	0.83	+0.4
4,542,527	-		PP_4031	0.83	+0.1
4,542,527	-		PP_4031	0.83	-0.1
4,542,527	-		PP_4031	0.83	+0.2
4,542,527	-		PP_4031	0.83	-0.3
4,542,703	+				+0.3
4,542,733	+				-0.4
4,542,734	-				-0.3
4,542,734	-				-0.0
4,542,734	-				-0.4
4,542,735	+				+0.3
4,542,736	-				+0.0
4,542,775	+				-0.8
4,542,775	+				-0.6
4,542,776	-				-0.1
4,542,867	+				-0.2
4,542,867	+				-0.3
4,542,903	+				+0.4
4,542,903	+				+0.1
4,543,044	+		PP_4032	0.20	+0.3
4,543,045	-		PP_4032	0.21	-0.0
4,543,045	-		PP_4032	0.21	-0.6
4,543,053	-		PP_4032	0.22	+0.0
4,543,094	+		PP_4032	0.29	+1.0
4,543,094	+		PP_4032	0.29	-0.4
4,543,150	+		PP_4032	0.40	-0.1
4,543,151	-		PP_4032	0.40	+0.2
4,543,151	-		PP_4032	0.40	-0.8
4,543,151	-		PP_4032	0.40	-0.3
4,543,151	-		PP_4032	0.40	-0.5
4,543,297	-		PP_4032	0.67	+0.2
4,543,309	-		PP_4032	0.69	+0.3
4,543,324	-		PP_4032	0.72	-1.4
4,543,440	+				+0.2
4,543,440	+				-0.5
4,543,441	-				-0.5
4,543,441	-				-0.5
4,543,441	-				-1.3
4,543,441	-				-0.5
4,543,441	-				-0.2
4,543,472	+				-1.5
4,543,472	+				-0.1
4,543,473	-				+0.5
4,543,509	+				-0.1
4,543,526	-				+0.1
4,543,662	+		PP_4033	0.10	-0.4
4,543,663	-		PP_4033	0.10	+0.3