Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3197 and PP_3198 are separated by 12 nucleotides PP_3198 and PP_3199 are separated by 139 nucleotides
PP_3197: PP_3197 - Glyoxalase family protein, at 3,626,779 to 3,627,720
_3197
PP_3198: PP_3198 - Ferredoxin, 2Fe-2S, at 3,627,733 to 3,628,071
_3198
PP_3199: PP_3199 - putative Monooxygenase, at 3,628,211 to 3,629,335
_3199
Position (kb)
3627
3628
3629 Strain fitness (log2 ratio)
-1
0
1
2 at 3626.755 kb on - strand at 3626.775 kb on - strand at 3626.784 kb on + strand at 3626.785 kb on - strand at 3626.785 kb on - strand at 3626.785 kb on - strand at 3626.855 kb on - strand at 3626.925 kb on + strand, within PP_3197 at 3627.163 kb on - strand, within PP_3197 at 3627.163 kb on - strand, within PP_3197 at 3627.259 kb on + strand, within PP_3197 at 3627.259 kb on + strand, within PP_3197 at 3627.441 kb on + strand, within PP_3197 at 3627.839 kb on + strand, within PP_3198 at 3627.934 kb on + strand, within PP_3198 at 3627.934 kb on + strand at 3627.935 kb on - strand, within PP_3198 at 3628.043 kb on + strand at 3628.044 kb on - strand at 3628.146 kb on + strand at 3628.216 kb on + strand at 3628.216 kb on + strand at 3628.217 kb on - strand at 3628.335 kb on - strand, within PP_3199 at 3628.403 kb on + strand, within PP_3199 at 3628.442 kb on + strand, within PP_3199 at 3628.442 kb on + strand, within PP_3199 at 3628.443 kb on - strand, within PP_3199 at 3628.443 kb on - strand, within PP_3199 at 3628.443 kb on - strand, within PP_3199 at 3628.665 kb on - strand, within PP_3199 at 3628.808 kb on + strand, within PP_3199 at 3628.809 kb on - strand, within PP_3199 at 3628.809 kb on - strand, within PP_3199 at 3628.862 kb on + strand, within PP_3199 at 3628.862 kb on + strand, within PP_3199
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2 remove 3,626,755 - +0.9 3,626,775 - -0.7 3,626,784 + -1.0 3,626,785 - +0.4 3,626,785 - +0.6 3,626,785 - +0.8 3,626,855 - +0.2 3,626,925 + PP_3197 0.15 -0.6 3,627,163 - PP_3197 0.41 +0.3 3,627,163 - PP_3197 0.41 -1.1 3,627,259 + PP_3197 0.51 -0.0 3,627,259 + PP_3197 0.51 +0.4 3,627,441 + PP_3197 0.70 +0.1 3,627,839 + PP_3198 0.31 -0.6 3,627,934 + PP_3198 0.59 -1.2 3,627,934 + +1.9 3,627,935 - PP_3198 0.60 +0.4 3,628,043 + +0.1 3,628,044 - -0.3 3,628,146 + -0.8 3,628,216 + -0.3 3,628,216 + +1.1 3,628,217 - -0.0 3,628,335 - PP_3199 0.11 +0.3 3,628,403 + PP_3199 0.17 -0.5 3,628,442 + PP_3199 0.21 +0.1 3,628,442 + PP_3199 0.21 +0.4 3,628,443 - PP_3199 0.21 -0.8 3,628,443 - PP_3199 0.21 +0.1 3,628,443 - PP_3199 0.21 +0.1 3,628,665 - PP_3199 0.40 +0.3 3,628,808 + PP_3199 0.53 -0.4 3,628,809 - PP_3199 0.53 +0.6 3,628,809 - PP_3199 0.53 -1.0 3,628,862 + PP_3199 0.58 +0.2 3,628,862 + PP_3199 0.58 +0.5
Or see this region's nucleotide sequence