Strain Fitness in Pseudomonas putida KT2440 around PP_1395

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
1,590,096	+		PP_1394	0.54	-0.3
1,590,130	+		PP_1394	0.56	-0.5
1,590,131	-		PP_1394	0.56	+0.4
1,590,153	-		PP_1394	0.57	+0.4
1,590,321	-		PP_1394	0.68	-0.3
1,590,373	+		PP_1394	0.71	+0.5
1,590,591	-		PP_1394	0.84	+0.0
1,590,603	-		PP_1394	0.85	+0.1
1,590,603	-		PP_1394	0.85	-0.5
1,590,626	-		PP_1394	0.86	+0.6
1,590,643	+		PP_1394	0.87	-0.3
1,590,643	+		PP_1394	0.87	+0.5
1,590,643	+		PP_1394	0.87	-0.2
1,590,643	+				+0.1
1,590,644	-		PP_1394	0.87	+0.1
1,590,644	-		PP_1394	0.87	+0.1
1,590,644	-		PP_1394	0.87	+0.7
1,590,644	-		PP_1394	0.87	+0.4
1,590,644	-		PP_1394	0.87	+0.4
1,590,644	-		PP_1394	0.87	+0.0
1,590,644	-		PP_1394	0.87	+0.0
1,591,068	-		PP_1395	0.10	-0.6
1,591,329	-		PP_1395	0.39	-0.3
1,591,478	+		PP_1395	0.55	-0.0
1,591,479	-		PP_1395	0.55	-0.2
1,591,479	-		PP_1395	0.55	-0.1
1,591,646	+		PP_1395	0.73	+0.5
1,591,646	+		PP_1395	0.73	-0.3
1,591,646	+		PP_1395	0.73	-0.6
1,591,646	+		PP_1395	0.73	+0.3
1,591,646	+		PP_1395	0.73	-0.6
1,591,646	+		PP_1395	0.73	-0.3
1,591,646	+		PP_1395	0.73	-0.5
1,591,646	+		PP_1395	0.73	+0.3
1,591,647	-		PP_1395	0.73	-0.3
1,591,647	-		PP_1395	0.73	-0.3
1,591,647	-		PP_1395	0.73	+0.5
1,591,647	-		PP_1395	0.73	+0.2
1,591,647	-		PP_1395	0.73	+0.0
1,591,647	-		PP_1395	0.73	+0.1
1,591,647	-		PP_1395	0.73	-0.4
1,591,647	-		PP_1395	0.73	+0.5
1,591,647	-		PP_1395	0.73	-0.0
1,591,647	-		PP_1395	0.73	-0.3
1,591,647	-		PP_1395	0.73	-1.0
1,591,667	+		PP_1395	0.75	-0.1
1,591,667	+		PP_1395	0.75	+0.9
1,591,667	+		PP_1395	0.75	+0.2
1,591,668	-		PP_1395	0.75	-0.5
1,591,741	+		PP_1395	0.83	+1.6
1,591,750	+		PP_1395	0.84	-0.3
1,591,763	+		PP_1395	0.86	-0.1
1,591,764	-		PP_1395	0.86	+0.1
1,591,775	+		PP_1395	0.87	-0.1
1,591,775	+		PP_1395	0.87	+0.2
1,591,776	-		PP_1395	0.87	+0.2
1,591,924	+				+0.8
1,591,924	+				+0.1
1,591,924	+				+0.3
1,591,925	-				-0.2
1,591,925	-				-0.2
1,591,925	-				-0.2
1,591,925	-				-0.1
1,591,925	-				+0.1
1,592,013	+				-0.8
1,592,013	+				-0.7
1,592,014	-				+0.3
1,592,104	+				-0.1
1,592,204	-		PP_1396	0.37	-0.0
1,592,204	-		PP_1396	0.37	-0.2
1,592,303	+		PP_1396	0.70	+0.5
1,592,303	+		PP_1396	0.70	-0.4
1,592,303	+		PP_1396	0.70	+0.1
1,592,303	+		PP_1396	0.70	-0.3
1,592,304	-		PP_1396	0.71	-0.6
1,592,377	+				+0.1
1,592,377	+				-0.1
1,592,377	+				+0.2
1,592,377	+				+0.1
1,592,378	-				+0.3
1,592,402	+				+0.2
1,592,403	-				+0.5
1,592,403	-				+0.2
1,592,447	+		PP_1397	0.15	-0.1
1,592,448	-		PP_1397	0.16	-0.3
1,592,448	-		PP_1397	0.16	+0.5
1,592,448	-		PP_1397	0.16	+0.2
1,592,720	-				-1.0
1,592,770	+		PP_1398	0.16	-0.2
1,592,770	+		PP_1398	0.16	-1.1
1,592,770	+		PP_1398	0.16	-0.1
1,592,771	-		PP_1398	0.16	-0.5
1,592,771	-		PP_1398	0.16	+0.8