Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0854 and PP_0855 are separated by 39 nucleotides PP_0855 and PP_0856 are separated by 0 nucleotides PP_0856 and PP_0857 are separated by 116 nucleotides
PP_0854: PP_0854 - Histidine--tRNA ligase, at 988,149 to 989,438
_0854
PP_0855: PP_0855 - conserved protein of unknown function, at 989,478 to 990,119
_0855
PP_0856: PP_0856 - conserved lipoprotein of unknown function, at 990,120 to 991,262
_0856
PP_0857: PP_0857 - GTPase Der, at 991,379 to 992,842
_0857
Position (kb) 990
991
992 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 989.436 kb on + strand at 989.471 kb on + strand at 989.471 kb on + strand at 989.472 kb on - strand at 989.604 kb on + strand, within PP_0855 at 989.605 kb on - strand, within PP_0855 at 989.745 kb on + strand, within PP_0855 at 989.746 kb on - strand, within PP_0855 at 989.755 kb on - strand, within PP_0855 at 989.890 kb on + strand, within PP_0855 at 990.104 kb on + strand at 990.117 kb on + strand at 990.117 kb on + strand at 990.118 kb on - strand at 990.118 kb on - strand at 990.160 kb on + strand at 990.161 kb on - strand at 990.288 kb on + strand, within PP_0856 at 990.288 kb on + strand, within PP_0856 at 990.288 kb on + strand, within PP_0856 at 990.289 kb on - strand, within PP_0856 at 990.289 kb on - strand, within PP_0856 at 990.323 kb on + strand, within PP_0856 at 990.323 kb on + strand, within PP_0856 at 990.327 kb on + strand, within PP_0856 at 990.327 kb on + strand, within PP_0856 at 990.328 kb on - strand, within PP_0856 at 990.431 kb on - strand, within PP_0856 at 990.435 kb on + strand, within PP_0856 at 990.728 kb on - strand, within PP_0856 at 990.855 kb on + strand, within PP_0856 at 990.855 kb on + strand, within PP_0856 at 990.936 kb on + strand, within PP_0856 at 990.969 kb on + strand, within PP_0856 at 991.086 kb on + strand, within PP_0856 at 991.150 kb on + strand at 991.266 kb on + strand at 991.266 kb on + strand at 991.266 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2 remove 989,436 + -0.2 989,471 + +0.6 989,471 + +0.3 989,472 - +0.5 989,604 + PP_0855 0.20 -1.1 989,605 - PP_0855 0.20 -0.3 989,745 + PP_0855 0.42 -0.4 989,746 - PP_0855 0.42 +1.1 989,755 - PP_0855 0.43 -1.1 989,890 + PP_0855 0.64 -0.2 990,104 + -0.2 990,117 + -1.6 990,117 + -1.3 990,118 - +1.2 990,118 - -1.9 990,160 + -0.9 990,161 - -0.3 990,288 + PP_0856 0.15 -1.5 990,288 + PP_0856 0.15 -0.3 990,288 + PP_0856 0.15 -0.2 990,289 - PP_0856 0.15 +1.7 990,289 - PP_0856 0.15 -0.9 990,323 + PP_0856 0.18 -0.3 990,323 + PP_0856 0.18 -1.9 990,327 + PP_0856 0.18 -0.1 990,327 + PP_0856 0.18 -1.5 990,328 - PP_0856 0.18 +0.1 990,431 - PP_0856 0.27 -0.3 990,435 + PP_0856 0.28 +1.7 990,728 - PP_0856 0.53 -0.3 990,855 + PP_0856 0.64 -1.5 990,855 + PP_0856 0.64 -1.1 990,936 + PP_0856 0.71 -1.6 990,969 + PP_0856 0.74 +3.7 991,086 + PP_0856 0.85 +0.7 991,150 + -2.5 991,266 + -0.6 991,266 + -0.5 991,266 + +0.1
Or see this region's nucleotide sequence