Strain Fitness in Pseudomonas putida KT2440 around PP_0135

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
142,458	+		PP_0134	0.49	-0.8
142,458	+		PP_0134	0.49	-1.3
142,458	+		PP_0134	0.49	-0.2
142,458	+		PP_0134	0.49	-0.2
142,459	-		PP_0134	0.49	-0.2
142,459	-		PP_0134	0.49	+0.2
142,459	-		PP_0134	0.49	+0.3
142,459	-		PP_0134	0.49	-0.5
142,460	+		PP_0134	0.49	-0.9
142,460	+		PP_0134	0.49	-0.2
142,460	+		PP_0134	0.49	-0.0
142,460	+		PP_0134	0.49	-0.5
142,461	-		PP_0134	0.49	-0.3
142,461	-		PP_0134	0.49	+0.9
142,461	-		PP_0134	0.49	+0.2
142,461	-		PP_0134	0.49	-0.6
142,461	-		PP_0134	0.49	+0.6
142,461	-		PP_0134	0.49	+0.7
142,461	-		PP_0134	0.49	-0.2
142,466	+		PP_0134	0.50	-0.7
142,466	+		PP_0134	0.50	-0.5
142,467	-		PP_0134	0.50	-0.2
142,564	+		PP_0134	0.62	+0.5
142,564	+		PP_0134	0.62	+0.1
142,565	-		PP_0134	0.62	+0.2
142,565	-		PP_0134	0.62	+0.2
142,565	-		PP_0134	0.62	+0.7
142,691	-		PP_0134	0.77	+0.3
142,691	-		PP_0134	0.77	+0.3
142,921	-				+0.3
142,921	-				-0.7
142,921	-				-0.4
143,009	+				+0.2
143,157	-		PP_5428	0.36	+0.9
143,158	+		PP_5428	0.36	-1.1
143,158	+		PP_5428	0.36	+0.8
143,158	+		PP_5428	0.36	+0.0
143,158	+		PP_5428	0.36	+1.4
143,159	-		PP_5428	0.36	+0.3
143,161	+		PP_5428	0.37	+0.4
143,161	+		PP_5428	0.37	-0.1
143,161	+		PP_5428	0.37	-1.2
143,162	-		PP_5428	0.37	-0.6
143,162	-		PP_5428	0.37	-0.3
143,383	+				-0.2
143,383	+				-0.3
143,383	+				-0.3
143,384	-				+0.1
143,384	-				+0.0
143,384	-				-0.9
143,384	-				+0.1
143,436	+		PP_0135	0.30	-0.7
143,436	+		PP_0135	0.30	-1.0
143,437	-		PP_0135	0.31	-0.3
143,467	+		PP_0135	0.49	-0.7
143,467	+		PP_0135	0.49	+0.1
143,467	+		PP_0135	0.49	-1.3
143,468	-		PP_0135	0.50	-1.2
143,468	-		PP_0135	0.50	-0.2
143,468	-		PP_0135	0.50	-0.2
143,514	+		PP_0135	0.78	-0.3
143,515	-		PP_0135	0.79	-0.5
143,525	+		PP_0135	0.85	-0.2
143,788	+		PP_0136	0.50	+0.6
143,788	+		PP_0136	0.50	-1.0
143,788	+		PP_0136	0.50	+0.1
143,788	+		PP_0136	0.50	-1.0
143,788	+		PP_0136	0.50	+0.1
143,789	-		PP_0136	0.51	+0.0
143,789	-		PP_0136	0.51	+0.7
143,789	-		PP_0136	0.51	-0.2
143,789	-		PP_0136	0.51	-0.6
143,789	-		PP_0136	0.51	-1.3
143,789	-		PP_0136	0.51	+0.3
143,831	-		PP_0136	0.59	-0.5
143,831	-		PP_0136	0.59	+0.1
143,831	-		PP_0136	0.59	-0.2
143,831	-		PP_0136	0.59	-0.5
143,875	+		PP_0136	0.69	-0.4
144,017	-				+0.2
144,064	+				+0.4
144,065	-				-0.4
144,065	-				-0.3
144,067	+				-0.5
144,067	+				+2.0
144,068	-				+0.4
144,068	-				-0.1
144,068	-				-0.4
144,085	-				+0.3
144,086	+				-1.6
144,086	+				-1.6
144,087	-				+0.4
144,099	+				-0.5
144,099	+				-0.2
144,099	+				-0.6
144,099	+				-0.4
144,099	+				+0.1
144,100	-				-0.8
144,100	-				-0.0
144,100	-				-0.2
144,160	+				-0.1
144,161	-				+0.2
144,178	+				+0.8
144,178	+				-0.1
144,179	-				-0.2
144,202	+				+0.2
144,203	-				-0.3
144,262	-				+0.1
144,294	+				+0.3
144,295	-				-1.5
144,295	-				-0.2
144,295	-				+0.2
144,295	-				-0.1
144,352	+				-0.3
144,352	+				-0.0
144,353	-				-0.3
144,386	+				+0.3
144,386	+				-0.1
144,386	+				-0.4
144,386	+				-0.3
144,387	-				+0.1
144,439	+				-0.5
144,439	+				-0.2
144,503	-				-0.5
144,545	-				+0.2