Strain Fitness in Pseudomonas putida KT2440 around PP_3353

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
3,789,616	+		PP_3352	0.43	-0.4
3,789,616	+		PP_3352	0.43	+0.4
3,789,617	-		PP_3352	0.43	-0.2
3,789,617	-		PP_3352	0.43	+0.1
3,789,628	+		PP_3352	0.44	-0.3
3,789,805	+		PP_3352	0.55	+0.5
3,789,806	-				+1.6
3,789,806	-		PP_3352	0.55	-0.1
3,789,806	-		PP_3352	0.55	-0.2
3,789,860	+		PP_3352	0.58	-0.5
3,789,860	+		PP_3352	0.58	-0.4
3,789,860	+		PP_3352	0.58	+0.4
3,789,860	+		PP_3352	0.58	+0.1
3,789,860	+		PP_3352	0.58	+0.5
3,789,861	-		PP_3352	0.58	-1.1
3,789,861	-		PP_3352	0.58	-0.5
3,789,861	-		PP_3352	0.58	+0.6
3,789,982	+		PP_3352	0.65	+0.2
3,789,983	-		PP_3352	0.65	+0.3
3,789,983	-		PP_3352	0.65	-0.5
3,789,983	-		PP_3352	0.65	+0.1
3,790,069	+		PP_3352	0.70	+0.5
3,790,102	+		PP_3352	0.72	-0.3
3,790,258	+		PP_3352	0.82	-0.0
3,790,259	-		PP_3352	0.82	+0.3
3,790,259	-				+1.0
3,790,396	+				-0.2
3,790,396	+				-0.2
3,790,396	+				+0.2
3,790,396	+				-0.1
3,790,397	-				-0.1
3,790,397	-				+0.0
3,790,397	-				+0.4
3,790,397	-				-0.1
3,790,397	-				+0.4
3,790,486	+				-0.2
3,790,487	-				-0.4
3,790,487	-				-0.3
3,790,656	-				-0.6
3,790,662	+		PP_3353	0.10	-0.0
3,790,663	-		PP_3353	0.10	-1.5
3,790,861	-		PP_3353	0.30	-0.3
3,791,088	+		PP_3353	0.52	-0.1
3,791,137	-		PP_3353	0.57	-0.5
3,791,216	+		PP_3353	0.64	-0.4
3,791,217	-		PP_3353	0.64	-0.0
3,791,264	+		PP_3353	0.69	-0.1
3,791,271	-		PP_3353	0.70	-0.1
3,791,990	-		PP_3354	0.24	+0.3
3,792,008	+		PP_3354	0.25	+0.4
3,792,008	+		PP_3354	0.25	-0.4
3,792,009	-		PP_3354	0.25	+0.6
3,792,009	-		PP_3354	0.25	+0.0
3,792,009	-		PP_3354	0.25	-1.4
3,792,065	+		PP_3354	0.29	+0.2
3,792,066	-		PP_3354	0.29	-0.1
3,792,066	-		PP_3354	0.29	-1.2
3,792,066	-		PP_3354	0.29	+0.3
3,792,258	+		PP_3354	0.40	+0.0
3,792,378	-		PP_3354	0.46	-0.1
3,792,493	+		PP_3354	0.53	-0.3
3,792,494	-		PP_3354	0.53	-0.6
3,792,523	+		PP_3354	0.55	-0.0
3,792,524	-		PP_3354	0.55	-0.7
3,792,558	+		PP_3354	0.57	+1.1
3,792,558	+		PP_3354	0.57	-0.0
3,792,559	-		PP_3354	0.57	+0.3
3,792,559	-		PP_3354	0.57	+0.2
3,792,559	-		PP_3354	0.57	-0.0
3,792,578	+		PP_3354	0.58	-0.1