Strain Fitness in Pseudomonas putida KT2440 around PP_2712

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
3,101,189	+		PP_2710	0.72	+0.1
3,101,234	+		PP_2710	0.78	-0.4
3,101,235	-		PP_2710	0.79	-0.4
3,101,417	-				-0.3
3,101,417	-				+0.6
3,101,417	-				-0.3
3,101,480	+		PP_2711	0.14	+0.7
3,101,664	+		PP_2711	0.37	-0.5
3,101,665	-		PP_2711	0.37	+0.2
3,101,680	+		PP_2711	0.39	+0.3
3,101,680	+		PP_2711	0.39	-0.1
3,101,680	+		PP_2711	0.39	+0.3
3,101,681	-		PP_2711	0.39	-0.1
3,101,781	+		PP_2711	0.51	-0.4
3,101,896	-		PP_2711	0.66	-0.5
3,101,972	+		PP_2711	0.75	+0.1
3,102,040	-		PP_2711	0.83	-0.6
3,102,072	-		PP_2711	0.87	-0.1
3,102,138	+				+0.3
3,102,139	-				+1.7
3,102,141	-				-0.2
3,102,284	+		PP_2712	0.18	-0.4
3,102,518	+		PP_2712	0.54	+0.3
3,102,539	+		PP_2712	0.57	-0.5
3,102,540	-				+0.0
3,102,540	-		PP_2712	0.58	-1.7
3,102,644	+		PP_2712	0.74	+0.1
3,102,691	+		PP_2712	0.81	-0.3
3,103,105	+		PP_2713	0.44	-0.4
3,103,106	-		PP_2713	0.44	-0.3
3,103,240	+		PP_2713	0.64	+0.0
3,103,241	-		PP_2713	0.64	+0.0
3,103,276	+		PP_2713	0.70	-0.0
3,103,277	-		PP_2713	0.70	-0.6
3,103,277	-		PP_2713	0.70	-0.0
3,103,316	+		PP_2713	0.76	-0.5
3,103,317	-		PP_2713	0.76	-0.1
3,103,317	-		PP_2713	0.76	-0.6
3,103,348	+		PP_2713	0.81	-0.1
3,103,348	+		PP_2713	0.81	-0.1
3,103,397	-		PP_2713	0.88	-0.5
3,103,397	-		PP_2713	0.88	+0.4
3,103,453	+				+0.2
3,103,550	+				+0.1
3,103,550	+				+0.1
3,103,554	+				-0.1
3,103,555	-				+0.3
3,103,555	-				+0.0
3,103,555	-				-0.6
3,103,585	+				-0.3
3,103,585	+				-0.1
3,103,585	+				-0.3
3,103,585	+				-0.3
3,103,586	-				-0.4
3,103,586	-				-0.2
3,103,586	-				-0.6
3,103,586	-				-0.0
3,103,586	-				-0.2
3,103,587	+				-0.3
3,103,587	+				-1.4
3,103,588	-				-0.4
3,103,671	+		PP_2714	0.15	+0.7
3,103,672	-		PP_2714	0.15	+0.1
3,103,784	+		PP_2714	0.23	+0.5
3,103,785	-		PP_2714	0.23	-0.1
3,103,785	-		PP_2714	0.23	-0.1
3,103,785	-		PP_2714	0.23	+0.0
3,103,785	-		PP_2714	0.23	-0.3