Strain Fitness in Pseudomonas putida KT2440 around PP_2437

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
2,782,608	+		PP_2436	0.12	+0.6
2,782,608	+		PP_2436	0.12	-0.2
2,782,737	+		PP_2436	0.26	-1.3
2,782,738	-		PP_2436	0.26	+0.0
2,782,801	-		PP_2436	0.33	+0.5
2,782,801	-		PP_2436	0.33	+0.2
2,782,828	+		PP_2436	0.36	-0.2
2,782,828	+		PP_2436	0.36	-0.3
2,782,829	-		PP_2436	0.36	-0.2
2,782,829	-		PP_2436	0.36	+0.2
2,782,829	-		PP_2436	0.36	-0.7
2,782,852	-		PP_2436	0.38	-0.4
2,782,911	+		PP_2436	0.45	-0.2
2,782,911	+		PP_2436	0.45	-0.3
2,783,326	+		PP_2436	0.90	-1.0
2,783,327	-		PP_2436	0.90	-0.3
2,783,371	-				+0.0
2,783,413	+				-0.2
2,783,414	-				+0.8
2,783,414	-				+0.3
2,783,414	-				-0.2
2,783,458	+				+0.3
2,783,459	-				-0.1
2,783,497	+				-0.1
2,783,521	+				+0.2
2,783,522	-				+0.2
2,783,537	+				+0.3
2,783,537	+				+0.5
2,783,555	+				-0.3
2,783,712	+		PP_2437	0.13	-0.0
2,783,713	-		PP_2437	0.13	-0.2
2,783,713	-		PP_2437	0.13	+0.1
2,783,767	+		PP_2437	0.18	+0.2
2,783,794	+		PP_2437	0.20	+0.0
2,784,414	+		PP_2437	0.70	+0.2
2,784,415	-		PP_2437	0.70	-0.0
2,784,415	-		PP_2437	0.70	+0.7
2,784,520	+		PP_2437	0.78	+0.2
2,784,520	+		PP_2437	0.78	+0.3
2,784,521	-		PP_2437	0.78	+0.3
2,784,521	-		PP_2437	0.78	-0.4
2,784,521	-		PP_2437	0.78	+0.8
2,784,626	+		PP_2437	0.87	-0.3
2,784,626	+		PP_2437	0.87	+0.4
2,784,626	+		PP_2437	0.87	+0.1
2,784,627	-		PP_2437	0.87	+0.5
2,784,627	-		PP_2437	0.87	+0.6
2,784,627	-		PP_2437	0.87	+0.4
2,784,667	+		PP_2437	0.90	-0.4
2,784,667	+		PP_2437	0.90	+0.1
2,784,667	+		PP_2437	0.90	-0.0
2,784,667	+		PP_2437	0.90	-0.1
2,784,668	-				-0.1
2,784,668	-				-0.1
2,784,668	-				+0.3
2,784,673	+				+0.4
2,784,673	+				+0.3
2,784,673	+				+0.1
2,784,673	+				-2.2
2,784,673	+				+0.3
2,784,673	+				-0.7
2,784,674	-				+0.4
2,784,674	-				+0.3
2,784,674	-				-0.2
2,784,674	-				+0.2
2,784,674	-				-0.2
2,784,674	-				-0.1
2,784,742	+				-1.2
2,784,742	+				-0.3
2,784,743	-				+0.2
2,784,757	+				+0.1
2,784,757	+				+0.2
2,784,758	-				+0.2
2,784,758	-				+0.8
2,784,758	-				+0.3
2,784,758	-				+0.7
2,784,758	-				-0.3
2,784,780	+				-0.4
2,784,867	-				-0.4
2,784,867	-				+0.0
2,784,873	+				+0.3
2,784,918	+				-0.0
2,784,919	-				-0.4
2,784,919	-				-0.2
2,784,940	+				+0.4
2,784,953	+				-0.1
2,784,953	+				-0.1
2,784,953	+				-0.1
2,784,953	+				-0.3
2,784,953	+				-0.7
2,784,953	+				+0.2
2,784,954	-				+0.3
2,784,954	-				-0.7
2,784,955	+				-0.3
2,784,955	+				+0.2
2,784,955	+				-0.1
2,784,955	+				-0.7
2,784,955	+				-0.1
2,784,955	+				-0.6
2,784,956	-				+0.6
2,784,956	-				-0.3
2,784,956	-				-1.1
2,785,024	+		PP_5522	0.27	-0.9
2,785,047	+		PP_5522	0.36	+0.5
2,785,120	-		PP_5522	0.64	-0.2
2,785,122	+		PP_5522	0.65	+0.5
2,785,123	-		PP_5522	0.65	+0.2
2,785,157	+		PP_5522	0.79	-0.4
2,785,157	+		PP_5522	0.79	-0.1
2,785,158	-		PP_5522	0.79	+0.5
2,785,161	+		PP_5522	0.80	-0.9
2,785,161	+		PP_5522	0.80	+0.4
2,785,162	-		PP_5522	0.81	+0.2
2,785,235	+				-0.0
2,785,236	-				+0.8
2,785,236	-				-0.2
2,785,244	+				-0.3
2,785,245	-				+0.8
2,785,245	-				-0.3
2,785,247	+		PP_5523	0.10	+0.6
2,785,248	-		PP_5523	0.11	+0.2
2,785,290	+		PP_5523	0.21	+0.1
2,785,397	+		PP_5523	0.49	+0.1
2,785,422	+		PP_5523	0.55	-0.5
2,785,422	+		PP_5523	0.55	-0.2
2,785,422	+		PP_5523	0.55	-0.9
2,785,423	-		PP_5523	0.55	+0.1
2,785,423	-		PP_5523	0.55	-0.1
2,785,427	+		PP_5523	0.56	-0.7
2,785,427	+		PP_5523	0.56	+0.3
2,785,428	-		PP_5523	0.57	-1.0
2,785,455	-		PP_5523	0.64	+0.5
2,785,455	-		PP_5523	0.64	-0.6
2,785,461	-		PP_5523	0.65	-0.1
2,785,521	+		PP_5523	0.81	+0.1
2,785,651	+				-0.2
2,785,657	+				+0.2
2,785,657	+				-0.3
2,785,671	-				+0.7