Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1635 and PP_1636 are separated by 73 nucleotides PP_1636 and PP_1637 overlap by 4 nucleotides PP_1637 and PP_1638 are separated by 117 nucleotides
PP_1635: PP_1635 - DNA-binding response regulator, at 1,832,012 to 1,832,662
_1635
PP_1636: PP_1636 - diacylglycerol kinase, at 1,832,736 to 1,833,101
_1636
PP_1637: PP_1637 - Transcriptional regulator, LysR family, at 1,833,098 to 1,834,024
_1637
PP_1638: PP_1638 - ferredoxin--NADP(+) reductase, at 1,834,142 to 1,834,921
_1638
Position (kb)
1833
1834
1835 Strain fitness (log2 ratio)
-1
0
1 at 1832.377 kb on - strand, within PP_1635 at 1832.534 kb on + strand, within PP_1635 at 1832.574 kb on + strand, within PP_1635 at 1833.161 kb on + strand at 1833.161 kb on + strand at 1833.161 kb on + strand at 1833.162 kb on - strand at 1833.195 kb on + strand, within PP_1637 at 1833.196 kb on - strand, within PP_1637 at 1833.196 kb on - strand, within PP_1637 at 1833.261 kb on - strand, within PP_1637 at 1833.376 kb on + strand at 1833.608 kb on + strand, within PP_1637 at 1833.687 kb on + strand, within PP_1637 at 1833.687 kb on + strand, within PP_1637 at 1833.687 kb on + strand, within PP_1637 at 1833.687 kb on + strand, within PP_1637 at 1833.687 kb on + strand, within PP_1637 at 1833.714 kb on + strand, within PP_1637 at 1833.812 kb on + strand, within PP_1637 at 1834.919 kb on + strand at 1834.919 kb on + strand at 1834.920 kb on - strand at 1834.965 kb on + strand at 1834.965 kb on + strand at 1834.966 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2 remove 1,832,377 - PP_1635 0.56 +0.6 1,832,534 + PP_1635 0.80 -0.4 1,832,574 + PP_1635 0.86 -0.7 1,833,161 + +0.4 1,833,161 + +0.5 1,833,161 + -0.3 1,833,162 - +0.4 1,833,195 + PP_1637 0.10 -0.4 1,833,196 - PP_1637 0.11 -0.0 1,833,196 - PP_1637 0.11 -0.9 1,833,261 - PP_1637 0.18 -0.1 1,833,376 + +0.4 1,833,608 + PP_1637 0.55 -0.3 1,833,687 + PP_1637 0.64 -0.3 1,833,687 + PP_1637 0.64 +0.2 1,833,687 + PP_1637 0.64 -0.3 1,833,687 + PP_1637 0.64 -0.3 1,833,687 + PP_1637 0.64 -0.4 1,833,714 + PP_1637 0.66 -0.6 1,833,812 + PP_1637 0.77 -0.4 1,834,919 + -0.3 1,834,919 + +0.2 1,834,920 - +0.1 1,834,965 + -0.2 1,834,965 + -0.0 1,834,966 - -0.0
Or see this region's nucleotide sequence