Strain Fitness in Pseudomonas putida KT2440 around PP_0615

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0614 and PP_0615 are separated by 106 nucleotidesPP_0615 and PP_0616 overlap by 1 nucleotidesPP_0616 and PP_0617 overlap by 1 nucleotides PP_0614: PP_0614 - putative N-carbamoyl-beta-alanine amidohydrolase/allantoine amidohydrolase 1, at 719,918 to 721,195 _0614 PP_0615: PP_0615 - Branched-chain amino acid ABC transporter, ATP-binding protein, at 721,302 to 721,997 _0615 PP_0616: PP_0616 - Branched-chain amino acid ABC transporter, ATP binding protein, at 721,997 to 722,749 _0616 PP_0617: PP_0617 - Branched-chain amino acid ABC transporter, permease protein, at 722,749 to 723,768 _0617 Position (kb) 721 722Strain fitness (log2 ratio) -1 0 1 2at 720.317 kb on + strand, within PP_0614at 720.318 kb on - strand, within PP_0614at 720.318 kb on - strand, within PP_0614at 720.590 kb on - strand, within PP_0614at 720.604 kb on - strand, within PP_0614at 720.646 kb on - strand, within PP_0614at 720.788 kb on + strand, within PP_0614at 720.788 kb on + strand, within PP_0614at 720.970 kb on + strand, within PP_0614at 720.971 kb on - strand, within PP_0614at 721.073 kb on + strandat 721.073 kb on + strandat 721.230 kb on - strandat 721.238 kb on - strandat 721.259 kb on + strandat 721.260 kb on - strandat 721.260 kb on - strandat 721.288 kb on + strandat 721.288 kb on + strandat 721.289 kb on - strandat 721.289 kb on - strandat 721.289 kb on - strandat 721.289 kb on - strandat 721.289 kb on - strandat 721.399 kb on + strand, within PP_0615at 721.399 kb on + strand, within PP_0615at 721.399 kb on + strand, within PP_0615at 721.399 kb on + strand, within PP_0615at 721.399 kb on + strand, within PP_0615at 721.400 kb on - strand, within PP_0615at 721.400 kb on - strand, within PP_0615at 721.431 kb on + strand, within PP_0615at 721.431 kb on + strand, within PP_0615at 721.431 kb on + strand, within PP_0615at 721.432 kb on - strand, within PP_0615at 721.432 kb on - strand, within PP_0615at 721.444 kb on - strand, within PP_0615at 721.660 kb on + strand, within PP_0615at 721.661 kb on - strand, within PP_0615at 721.661 kb on - strand, within PP_0615at 721.661 kb on - strand, within PP_0615at 721.661 kb on - strand, within PP_0615at 721.661 kb on - strand, within PP_0615at 721.782 kb on - strand, within PP_0615at 721.908 kb on + strand, within PP_0615at 721.966 kb on + strandat 721.966 kb on + strandat 721.969 kb on + strandat 721.969 kb on + strandat 721.970 kb on - strandat 721.970 kb on - strandat 721.970 kb on - strandat 722.016 kb on + strandat 722.017 kb on - strandat 722.144 kb on + strand, within PP_0616at 722.144 kb on + strand, within PP_0616at 722.144 kb on + strand, within PP_0616at 722.145 kb on - strand, within PP_0616at 722.152 kb on + strand, within PP_0616at 722.152 kb on + strand, within PP_0616at 722.153 kb on - strand, within PP_0616at 722.739 kb on + strandat 722.739 kb on + strandat 722.740 kb on - strandat 722.740 kb on - strandat 722.750 kb on + strandat 722.750 kb on + strandat 722.751 kb on - strandat 722.751 kb on - strandat 722.751 kb on - strandat 722.751 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
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720,317 + PP_0614 0.31 -0.1
720,318 - PP_0614 0.31 +0.1
720,318 - PP_0614 0.31 +0.1
720,590 - PP_0614 0.53 -0.4
720,604 - PP_0614 0.54 -0.4
720,646 - PP_0614 0.57 +1.9
720,788 + PP_0614 0.68 +0.2
720,788 + PP_0614 0.68 +0.1
720,970 + PP_0614 0.82 +0.4
720,971 - PP_0614 0.82 +0.0
721,073 + +0.4
721,073 + +1.4
721,230 - -0.1
721,238 - +0.2
721,259 + +0.4
721,260 - -0.4
721,260 - +0.1
721,288 + -0.2
721,288 + +0.1
721,289 - -0.1
721,289 - +0.3
721,289 - +0.7
721,289 - +0.5
721,289 - -0.3
721,399 + PP_0615 0.14 -0.1
721,399 + PP_0615 0.14 +0.2
721,399 + PP_0615 0.14 -0.0
721,399 + PP_0615 0.14 +0.7
721,399 + PP_0615 0.14 -0.4
721,400 - PP_0615 0.14 -1.0
721,400 - PP_0615 0.14 +0.6
721,431 + PP_0615 0.19 -0.3
721,431 + PP_0615 0.19 +0.1
721,431 + PP_0615 0.19 +0.4
721,432 - PP_0615 0.19 +0.4
721,432 - PP_0615 0.19 -1.2
721,444 - PP_0615 0.20 -0.3
721,660 + PP_0615 0.51 -0.4
721,661 - PP_0615 0.52 +0.2
721,661 - PP_0615 0.52 +0.7
721,661 - PP_0615 0.52 -0.6
721,661 - PP_0615 0.52 -0.1
721,661 - PP_0615 0.52 -0.6
721,782 - PP_0615 0.69 -0.6
721,908 + PP_0615 0.87 +0.4
721,966 + +0.1
721,966 + -0.1
721,969 + -0.2
721,969 + +0.2
721,970 - +0.4
721,970 - -0.8
721,970 - +0.3
722,016 + -0.1
722,017 - -0.2
722,144 + PP_0616 0.20 -0.0
722,144 + PP_0616 0.20 -0.1
722,144 + PP_0616 0.20 +0.5
722,145 - PP_0616 0.20 +0.0
722,152 + PP_0616 0.21 -0.2
722,152 + PP_0616 0.21 -0.5
722,153 - PP_0616 0.21 +0.2
722,739 + +0.2
722,739 + -0.0
722,740 - -0.5
722,740 - -0.1
722,750 + -0.1
722,750 + +0.4
722,751 - +0.1
722,751 - +0.0
722,751 - +0.2
722,751 - -0.1

Or see this region's nucleotide sequence