Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 500 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4240 and PP_5668 overlap by 4 nucleotides PP_5668 and PP_5669 overlap by 1 nucleotides PP_5669 and PP_4242 are separated by 228 nucleotides
PP_4240: PP_4240 - putative Microcin b17 processing protein mcbd, at 4,814,433 to 4,815,635
_4240
PP_5668: PP_5668 - conserved membrane protein of unknown function, at 4,815,632 to 4,816,375
_5668
PP_5669: PP_5669 - ABC transporter related, at 4,816,375 to 4,817,076
_5669
PP_4242: PP_4242 - conserved protein of unknown function with Rhs repeat-associated core domain, at 4,817,305 to 4,818,099
_4242
Position (kb)
4815
4816
4817 Strain fitness (log2 ratio)
-2
-1
0
1 at 4814.656 kb on + strand, within PP_4240 at 4814.772 kb on + strand, within PP_4240 at 4814.864 kb on + strand, within PP_4240 at 4814.897 kb on + strand, within PP_4240 at 4815.084 kb on - strand, within PP_4240 at 4815.125 kb on - strand, within PP_4240 at 4815.130 kb on - strand, within PP_4240 at 4815.130 kb on - strand, within PP_4240 at 4815.181 kb on - strand, within PP_4240 at 4815.657 kb on + strand at 4815.756 kb on + strand, within PP_5668 at 4815.757 kb on - strand, within PP_5668 at 4815.797 kb on + strand, within PP_5668 at 4815.797 kb on + strand, within PP_5668 at 4815.797 kb on + strand, within PP_5668 at 4815.821 kb on + strand, within PP_5668 at 4815.822 kb on - strand, within PP_5668 at 4815.822 kb on - strand, within PP_5668 at 4815.827 kb on + strand, within PP_5668 at 4815.828 kb on - strand, within PP_5668 at 4815.828 kb on - strand, within PP_5668 at 4815.866 kb on + strand, within PP_5668 at 4815.911 kb on + strand, within PP_5668 at 4815.991 kb on - strand, within PP_5668 at 4815.991 kb on - strand, within PP_5668 at 4816.047 kb on - strand, within PP_5668 at 4816.074 kb on - strand, within PP_5668 at 4816.389 kb on + strand at 4816.452 kb on - strand, within PP_5669 at 4816.617 kb on + strand, within PP_5669 at 4816.727 kb on - strand, within PP_5669 at 4816.765 kb on + strand, within PP_5669 at 4816.783 kb on + strand, within PP_5669 at 4816.808 kb on + strand, within PP_5669 at 4816.871 kb on - strand, within PP_5669 at 4816.872 kb on + strand, within PP_5669 at 4816.872 kb on + strand, within PP_5669 at 4816.873 kb on - strand, within PP_5669 at 4816.873 kb on - strand, within PP_5669 at 4816.874 kb on + strand, within PP_5669 at 4816.922 kb on + strand, within PP_5669 at 4816.970 kb on - strand, within PP_5669 at 4817.062 kb on - strand at 4817.272 kb on - strand at 4817.275 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 500 uM; timepoint 2 remove 4,814,656 + PP_4240 0.19 +0.4 4,814,772 + PP_4240 0.28 +0.4 4,814,864 + PP_4240 0.36 +0.0 4,814,897 + PP_4240 0.39 +0.5 4,815,084 - PP_4240 0.54 -0.6 4,815,125 - PP_4240 0.58 -0.8 4,815,130 - PP_4240 0.58 -0.7 4,815,130 - PP_4240 0.58 +0.7 4,815,181 - PP_4240 0.62 -0.2 4,815,657 + -1.1 4,815,756 + PP_5668 0.17 -0.6 4,815,757 - PP_5668 0.17 -0.2 4,815,797 + PP_5668 0.22 +0.0 4,815,797 + PP_5668 0.22 -1.3 4,815,797 + PP_5668 0.22 -0.9 4,815,821 + PP_5668 0.25 +0.2 4,815,822 - PP_5668 0.26 +0.6 4,815,822 - PP_5668 0.26 -0.3 4,815,827 + PP_5668 0.26 -1.8 4,815,828 - PP_5668 0.26 +0.5 4,815,828 - PP_5668 0.26 +0.7 4,815,866 + PP_5668 0.31 -0.1 4,815,911 + PP_5668 0.38 +0.0 4,815,991 - PP_5668 0.48 -0.1 4,815,991 - PP_5668 0.48 +0.3 4,816,047 - PP_5668 0.56 -0.3 4,816,074 - PP_5668 0.59 +0.0 4,816,389 + +0.5 4,816,452 - PP_5669 0.11 +0.9 4,816,617 + PP_5669 0.34 -0.2 4,816,727 - PP_5669 0.50 -0.1 4,816,765 + PP_5669 0.56 -0.9 4,816,783 + PP_5669 0.58 +0.6 4,816,808 + PP_5669 0.62 -0.1 4,816,871 - PP_5669 0.71 -0.0 4,816,872 + PP_5669 0.71 -0.1 4,816,872 + PP_5669 0.71 +0.6 4,816,873 - PP_5669 0.71 -0.4 4,816,873 - PP_5669 0.71 +0.3 4,816,874 + PP_5669 0.71 -0.6 4,816,922 + PP_5669 0.78 +0.5 4,816,970 - PP_5669 0.85 -0.2 4,817,062 - +1.1 4,817,272 - -0.3 4,817,275 + +0.7
Or see this region's nucleotide sequence