Strain Fitness in Pseudomonas putida KT2440 around PP_3727

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 1
4,251,379	+		PP_3726	0.18	-0.2
4,251,783	-		PP_3726	0.69	-1.1
4,252,119	+				-0.5
4,252,119	+				-0.7
4,252,120	-				+0.3
4,252,150	-				+0.2
4,252,150	-				+0.4
4,252,178	-				+0.5
4,252,178	-				-0.1
4,252,203	+				-0.1
4,252,330	+				-0.7
4,252,339	+				-0.8
4,252,340	-				+0.2
4,252,369	+				+0.3
4,252,456	+		PP_3727	0.13	+0.3
4,252,471	+		PP_3727	0.14	-0.5
4,252,471	+		PP_3727	0.14	+0.1
4,252,471	+		PP_3727	0.14	+0.4
4,252,742	+		PP_3727	0.33	+0.7
4,252,743	-		PP_3727	0.33	-0.9
4,252,756	+		PP_3727	0.34	-0.7
4,252,756	+		PP_3727	0.34	-0.2
4,252,756	+		PP_3727	0.34	+0.2
4,252,756	+		PP_3727	0.34	+0.4
4,252,756	+		PP_3727	0.34	-0.0
4,252,757	-		PP_3727	0.34	-0.5
4,252,757	-		PP_3727	0.34	-0.0
4,252,757	-		PP_3727	0.34	+0.3
4,252,924	+		PP_3727	0.46	-0.6
4,252,924	+		PP_3727	0.46	-0.2
4,252,924	+		PP_3727	0.46	-0.2
4,252,924	+		PP_3727	0.46	-0.7
4,252,924	+		PP_3727	0.46	-0.2
4,252,925	-		PP_3727	0.46	-0.1
4,252,925	-		PP_3727	0.46	+0.1
4,252,925	-		PP_3727	0.46	-0.1
4,253,036	-		PP_3727	0.54	-0.0
4,253,036	-		PP_3727	0.54	-0.5
4,253,218	+		PP_3727	0.67	-0.4
4,253,358	+		PP_3727	0.77	+0.4
4,253,358	+		PP_3727	0.77	+0.1
4,253,383	+		PP_3727	0.79	-0.2
4,253,383	+		PP_3727	0.79	-0.3
4,253,422	+		PP_3727	0.82	-0.6
4,253,422	+		PP_3727	0.82	+0.0
4,253,422	+		PP_3727	0.82	+0.3
4,253,423	-		PP_3727	0.82	+0.3
4,253,500	+		PP_3727	0.87	-1.8
4,253,500	+		PP_3727	0.87	-0.6
4,253,501	-		PP_3727	0.87	-0.2
4,253,525	-		PP_3727	0.89	-0.2
4,253,525	-		PP_3727	0.89	+0.2
4,253,525	-		PP_3727	0.89	-0.5
4,253,565	+				-0.1
4,253,565	+				+0.1
4,253,565	+				-0.2
4,253,737	-				-0.5
4,253,749	+				-0.5
4,253,750	-				+0.3
4,253,764	+				-0.3
4,253,765	-				-0.4
4,253,765	-				+0.1
4,253,803	+				-0.2
4,253,804	-				+0.3
4,253,818	+				-0.1
4,253,818	+				-1.8
4,253,818	+				-0.0
4,253,818	+				+0.3
4,253,818	+				+0.0
4,253,819	-				+0.7
4,253,819	-				-0.0
4,253,831	+				-0.7
4,253,872	-				-0.1
4,253,917	+				+0.1
4,253,917	+				+0.1
4,253,918	-				+0.4
4,253,959	+				-0.4
4,253,959	+				+0.1
4,253,960	-				-0.1
4,254,242	-		PP_3728	0.16	-0.4
4,254,292	+		PP_3728	0.19	-0.1
4,254,303	+		PP_3728	0.19	-0.1
4,254,304	-		PP_3728	0.19	+0.0
4,254,393	+		PP_3728	0.23	-0.0
4,254,393	+		PP_3728	0.23	+0.2
4,254,394	-		PP_3728	0.23	+0.1
4,254,514	+		PP_3728	0.28	+0.0