Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4487 and PP_4488 are separated by 268 nucleotides PP_4488 and PP_4489 are separated by 152 nucleotides PP_4489 and PP_4490 are separated by 257 nucleotides
PP_4487: PP_4487 - acetyl-CoA synthetase, at 5,097,301 to 5,099,391
_4487
PP_4488: PP_4488 - conserved exported protein of unknown function, at 5,099,660 to 5,099,914
_4488
PP_4489: PP_4489 - sigma-54 dependent transcriptional regulator, at 5,100,067 to 5,101,626
_4489
PP_4490: PP_4490 - phenylalanine-4-hydroxylase, at 5,101,884 to 5,102,672
_4490
Position (kb) 5100
5101
5102 Strain fitness (log2 ratio) -1
0
1 at 5099.127 kb on + strand, within PP_4487 at 5099.180 kb on + strand, within PP_4487 at 5099.180 kb on + strand, within PP_4487 at 5099.181 kb on - strand, within PP_4487 at 5099.181 kb on - strand, within PP_4487 at 5099.181 kb on - strand, within PP_4487 at 5099.192 kb on + strand at 5099.192 kb on + strand at 5099.192 kb on + strand at 5099.192 kb on + strand at 5099.192 kb on + strand at 5099.193 kb on - strand at 5099.193 kb on - strand at 5099.193 kb on - strand at 5099.193 kb on - strand at 5099.215 kb on - strand at 5099.243 kb on + strand at 5099.279 kb on + strand at 5099.280 kb on - strand at 5099.280 kb on - strand at 5099.280 kb on - strand at 5099.282 kb on + strand at 5099.282 kb on + strand at 5099.283 kb on - strand at 5099.283 kb on - strand at 5099.283 kb on - strand at 5099.294 kb on + strand at 5099.309 kb on - strand at 5099.309 kb on - strand at 5099.315 kb on - strand at 5099.345 kb on + strand at 5099.348 kb on + strand at 5099.565 kb on + strand at 5099.602 kb on - strand at 5099.642 kb on + strand at 5099.643 kb on - strand at 5099.650 kb on + strand at 5099.650 kb on + strand at 5099.650 kb on + strand at 5099.651 kb on - strand at 5099.671 kb on - strand at 5099.676 kb on + strand at 5099.676 kb on + strand at 5099.676 kb on + strand at 5099.677 kb on - strand at 5099.683 kb on - strand at 5099.683 kb on - strand at 5099.754 kb on - strand, within PP_4488 at 5099.759 kb on + strand, within PP_4488 at 5099.759 kb on + strand, within PP_4488 at 5099.759 kb on + strand, within PP_4488 at 5099.843 kb on + strand, within PP_4488 at 5099.843 kb on + strand, within PP_4488 at 5099.843 kb on + strand, within PP_4488 at 5099.844 kb on - strand, within PP_4488 at 5099.844 kb on - strand, within PP_4488 at 5099.844 kb on - strand, within PP_4488 at 5099.844 kb on - strand, within PP_4488 at 5099.844 kb on - strand, within PP_4488 at 5099.915 kb on + strand at 5099.915 kb on + strand at 5099.916 kb on - strand at 5099.946 kb on - strand at 5099.973 kb on + strand at 5099.974 kb on - strand at 5099.974 kb on - strand at 5099.974 kb on - strand at 5099.974 kb on - strand at 5099.974 kb on - strand at 5099.998 kb on + strand at 5099.998 kb on + strand at 5099.999 kb on - strand at 5099.999 kb on - strand at 5099.999 kb on - strand at 5099.999 kb on - strand at 5100.001 kb on + strand at 5100.002 kb on - strand at 5100.002 kb on - strand at 5100.002 kb on - strand at 5100.002 kb on - strand at 5100.068 kb on + strand at 5100.267 kb on - strand, within PP_4489 at 5100.314 kb on - strand, within PP_4489 at 5100.352 kb on + strand, within PP_4489 at 5100.358 kb on - strand, within PP_4489 at 5100.371 kb on + strand, within PP_4489 at 5100.372 kb on - strand, within PP_4489 at 5100.514 kb on + strand, within PP_4489 at 5100.515 kb on - strand, within PP_4489 at 5100.515 kb on - strand, within PP_4489 at 5100.515 kb on - strand, within PP_4489 at 5100.531 kb on - strand, within PP_4489 at 5100.531 kb on - strand, within PP_4489 at 5100.611 kb on + strand, within PP_4489 at 5100.627 kb on + strand, within PP_4489 at 5100.627 kb on + strand, within PP_4489 at 5100.627 kb on + strand, within PP_4489 at 5100.673 kb on + strand, within PP_4489 at 5100.673 kb on + strand, within PP_4489 at 5100.673 kb on + strand, within PP_4489 at 5100.673 kb on + strand, within PP_4489 at 5100.674 kb on - strand, within PP_4489 at 5100.674 kb on - strand, within PP_4489 at 5100.674 kb on - strand, within PP_4489 at 5100.674 kb on - strand, within PP_4489 at 5101.046 kb on + strand, within PP_4489 at 5101.046 kb on + strand, within PP_4489 at 5101.046 kb on + strand, within PP_4489 at 5101.046 kb on + strand, within PP_4489 at 5101.046 kb on + strand, within PP_4489 at 5101.046 kb on + strand, within PP_4489 at 5101.046 kb on + strand, within PP_4489 at 5101.047 kb on - strand, within PP_4489 at 5101.047 kb on - strand, within PP_4489 at 5101.047 kb on - strand, within PP_4489 at 5101.047 kb on - strand, within PP_4489 at 5101.050 kb on + strand, within PP_4489 at 5101.050 kb on + strand, within PP_4489 at 5101.051 kb on - strand, within PP_4489 at 5101.234 kb on + strand, within PP_4489 at 5101.235 kb on - strand, within PP_4489 at 5101.235 kb on - strand, within PP_4489 at 5101.238 kb on + strand, within PP_4489 at 5101.238 kb on + strand, within PP_4489 at 5101.419 kb on + strand, within PP_4489 at 5101.502 kb on + strand at 5101.502 kb on + strand at 5101.503 kb on - strand at 5101.543 kb on + strand at 5101.544 kb on - strand at 5101.544 kb on - strand at 5101.620 kb on + strand at 5101.681 kb on + strand at 5101.681 kb on + strand at 5101.828 kb on + strand at 5101.829 kb on - strand at 5101.853 kb on + strand at 5101.854 kb on - strand at 5101.854 kb on - strand at 5101.854 kb on - strand at 5101.854 kb on - strand at 5101.854 kb on - strand at 5101.899 kb on + strand at 5101.899 kb on + strand at 5101.900 kb on - strand at 5101.900 kb on - strand at 5101.900 kb on - strand at 5101.900 kb on - strand at 5101.931 kb on + strand at 5101.932 kb on - strand at 5102.019 kb on + strand, within PP_4490 at 5102.221 kb on - strand, within PP_4490 at 5102.308 kb on - strand, within PP_4490 at 5102.350 kb on - strand, within PP_4490 at 5102.364 kb on + strand, within PP_4490 at 5102.419 kb on - strand, within PP_4490 at 5102.419 kb on - strand, within PP_4490 at 5102.457 kb on + strand, within PP_4490 at 5102.457 kb on + strand, within PP_4490 at 5102.458 kb on - strand, within PP_4490 at 5102.458 kb on - strand, within PP_4490 at 5102.513 kb on + strand, within PP_4490 at 5102.513 kb on + strand, within PP_4490 at 5102.514 kb on - strand, within PP_4490 at 5102.514 kb on - strand, within PP_4490 at 5102.514 kb on - strand, within PP_4490 at 5102.514 kb on - strand, within PP_4490 at 5102.548 kb on - strand, within PP_4490 at 5102.555 kb on - strand, within PP_4490 at 5102.615 kb on + strand at 5102.615 kb on + strand at 5102.615 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 1 remove 5,099,127 + PP_4487 0.87 -0.1 5,099,180 + PP_4487 0.90 +0.8 5,099,180 + PP_4487 0.90 -1.0 5,099,181 - PP_4487 0.90 +0.4 5,099,181 - PP_4487 0.90 +0.2 5,099,181 - PP_4487 0.90 +0.0 5,099,192 + +0.2 5,099,192 + +0.9 5,099,192 + -0.4 5,099,192 + -0.2 5,099,192 + -0.0 5,099,193 - +0.6 5,099,193 - +0.1 5,099,193 - +0.6 5,099,193 - +0.0 5,099,215 - +0.2 5,099,243 + +0.2 5,099,279 + -0.0 5,099,280 - +0.4 5,099,280 - +0.0 5,099,280 - +0.1 5,099,282 + +0.1 5,099,282 + -0.2 5,099,283 - +0.1 5,099,283 - -0.3 5,099,283 - -0.3 5,099,294 + +0.3 5,099,309 - -0.3 5,099,309 - -1.6 5,099,315 - -0.5 5,099,345 + -0.2 5,099,348 + +0.4 5,099,565 + -0.0 5,099,602 - -0.2 5,099,642 + +0.2 5,099,643 - +0.1 5,099,650 + +0.1 5,099,650 + -0.4 5,099,650 + -0.1 5,099,651 - -0.0 5,099,671 - +0.1 5,099,676 + -0.3 5,099,676 + -0.4 5,099,676 + -0.4 5,099,677 - +0.2 5,099,683 - +0.1 5,099,683 - +0.1 5,099,754 - PP_4488 0.37 -0.0 5,099,759 + PP_4488 0.39 -0.3 5,099,759 + PP_4488 0.39 -0.3 5,099,759 + PP_4488 0.39 -0.6 5,099,843 + PP_4488 0.72 +1.1 5,099,843 + PP_4488 0.72 +0.1 5,099,843 + PP_4488 0.72 +0.2 5,099,844 - PP_4488 0.72 -0.2 5,099,844 - PP_4488 0.72 +0.3 5,099,844 - PP_4488 0.72 -0.9 5,099,844 - PP_4488 0.72 -0.3 5,099,844 - PP_4488 0.72 -0.3 5,099,915 + -0.0 5,099,915 + -0.1 5,099,916 - -0.5 5,099,946 - -0.4 5,099,973 + -0.4 5,099,974 - -0.3 5,099,974 - -0.4 5,099,974 - -0.6 5,099,974 - -0.3 5,099,974 - -0.5 5,099,998 + -0.2 5,099,998 + -0.1 5,099,999 - -0.5 5,099,999 - -0.5 5,099,999 - +0.3 5,099,999 - +0.0 5,100,001 + -0.5 5,100,002 - -0.1 5,100,002 - -0.2 5,100,002 - +0.5 5,100,002 - +0.1 5,100,068 + -0.1 5,100,267 - PP_4489 0.13 -0.2 5,100,314 - PP_4489 0.16 -0.2 5,100,352 + PP_4489 0.18 +0.5 5,100,358 - PP_4489 0.19 +0.3 5,100,371 + PP_4489 0.19 +0.2 5,100,372 - PP_4489 0.20 -0.3 5,100,514 + PP_4489 0.29 +0.6 5,100,515 - PP_4489 0.29 +0.0 5,100,515 - PP_4489 0.29 -0.4 5,100,515 - PP_4489 0.29 -0.2 5,100,531 - PP_4489 0.30 +0.2 5,100,531 - PP_4489 0.30 -0.8 5,100,611 + PP_4489 0.35 +0.1 5,100,627 + PP_4489 0.36 +0.2 5,100,627 + PP_4489 0.36 -0.2 5,100,627 + PP_4489 0.36 +0.0 5,100,673 + PP_4489 0.39 -0.3 5,100,673 + PP_4489 0.39 +0.1 5,100,673 + PP_4489 0.39 -0.3 5,100,673 + PP_4489 0.39 +0.3 5,100,674 - PP_4489 0.39 +0.0 5,100,674 - PP_4489 0.39 +0.3 5,100,674 - PP_4489 0.39 -0.3 5,100,674 - PP_4489 0.39 +0.4 5,101,046 + PP_4489 0.63 -0.6 5,101,046 + PP_4489 0.63 +0.1 5,101,046 + PP_4489 0.63 +0.1 5,101,046 + PP_4489 0.63 -0.5 5,101,046 + PP_4489 0.63 +0.2 5,101,046 + PP_4489 0.63 +0.2 5,101,046 + PP_4489 0.63 -0.7 5,101,047 - PP_4489 0.63 -0.1 5,101,047 - PP_4489 0.63 -0.7 5,101,047 - PP_4489 0.63 -0.1 5,101,047 - PP_4489 0.63 -0.2 5,101,050 + PP_4489 0.63 +0.3 5,101,050 + PP_4489 0.63 +0.1 5,101,051 - PP_4489 0.63 -0.3 5,101,234 + PP_4489 0.75 -0.1 5,101,235 - PP_4489 0.75 +0.1 5,101,235 - PP_4489 0.75 +0.0 5,101,238 + PP_4489 0.75 -0.1 5,101,238 + PP_4489 0.75 -0.1 5,101,419 + PP_4489 0.87 -0.4 5,101,502 + +0.3 5,101,502 + +0.4 5,101,503 - +0.3 5,101,543 + -0.7 5,101,544 - -0.3 5,101,544 - -0.2 5,101,620 + -0.3 5,101,681 + -0.3 5,101,681 + -0.5 5,101,828 + -0.3 5,101,829 - -0.1 5,101,853 + +0.4 5,101,854 - -0.7 5,101,854 - -0.1 5,101,854 - -0.2 5,101,854 - +0.0 5,101,854 - -0.4 5,101,899 + -0.2 5,101,899 + -0.0 5,101,900 - +0.3 5,101,900 - +0.1 5,101,900 - -0.3 5,101,900 - +0.1 5,101,931 + +0.1 5,101,932 - +0.4 5,102,019 + PP_4490 0.17 -0.2 5,102,221 - PP_4490 0.43 +0.2 5,102,308 - PP_4490 0.54 -0.5 5,102,350 - PP_4490 0.59 +0.1 5,102,364 + PP_4490 0.61 -0.0 5,102,419 - PP_4490 0.68 -0.1 5,102,419 - PP_4490 0.68 +0.1 5,102,457 + PP_4490 0.73 +0.0 5,102,457 + PP_4490 0.73 -0.1 5,102,458 - PP_4490 0.73 -0.7 5,102,458 - PP_4490 0.73 +0.7 5,102,513 + PP_4490 0.80 +0.1 5,102,513 + PP_4490 0.80 +0.2 5,102,514 - PP_4490 0.80 -0.4 5,102,514 - PP_4490 0.80 -0.3 5,102,514 - PP_4490 0.80 +0.4 5,102,514 - PP_4490 0.80 -0.0 5,102,548 - PP_4490 0.84 -0.5 5,102,555 - PP_4490 0.85 -0.1 5,102,615 + +0.6 5,102,615 + -0.0 5,102,615 + -0.1
Or see this region's nucleotide sequence