Strain Fitness in Pseudomonas putida KT2440 around PP_0820

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0818 and PP_0819 are separated by 161 nucleotidesPP_0819 and PP_0820 are separated by 192 nucleotidesPP_0820 and PP_5460 are separated by 86 nucleotidesPP_5460 and PP_0821 overlap by 4 nucleotides PP_0818: PP_0818 - conserved protein of unknown function, at 956,681 to 957,808 _0818 PP_0819: PP_0819 - conserved ferredoxin-like protein of unknown function, at 957,970 to 958,353 _0819 PP_0820: PP_0820 - GCN5-related N-acetyltransferase, at 958,546 to 959,109 _0820 PP_5460: PP_5460 - conserved membrane protein of unknown function, at 959,196 to 959,735 _5460 PP_0821: PP_0821 - conserved exported protein of unknown function, at 959,732 to 960,142 _0821 Position (kb) 958 959 960Strain fitness (log2 ratio) -1 0 1at 957.596 kb on + strand, within PP_0818at 957.596 kb on + strand, within PP_0818at 957.629 kb on + strand, within PP_0818at 957.630 kb on - strand, within PP_0818at 957.630 kb on - strand, within PP_0818at 957.646 kb on + strand, within PP_0818at 957.646 kb on + strand, within PP_0818at 957.646 kb on + strand, within PP_0818at 957.646 kb on + strand, within PP_0818at 957.646 kb on + strand, within PP_0818at 957.646 kb on + strand, within PP_0818at 957.646 kb on + strand, within PP_0818at 957.653 kb on + strand, within PP_0818at 957.654 kb on - strand, within PP_0818at 957.654 kb on - strand, within PP_0818at 957.660 kb on + strand, within PP_0818at 957.712 kb on + strandat 957.744 kb on - strandat 957.762 kb on + strandat 957.762 kb on + strandat 957.763 kb on - strandat 957.763 kb on - strandat 957.791 kb on + strandat 957.893 kb on - strandat 957.979 kb on + strandat 957.979 kb on + strandat 957.980 kb on - strandat 957.980 kb on - strandat 957.980 kb on - strandat 957.981 kb on + strandat 957.982 kb on - strandat 957.982 kb on - strandat 958.064 kb on + strand, within PP_0819at 958.065 kb on - strand, within PP_0819at 958.085 kb on + strand, within PP_0819at 958.086 kb on - strand, within PP_0819at 958.086 kb on - strand, within PP_0819at 958.086 kb on - strand, within PP_0819at 958.086 kb on - strand, within PP_0819at 958.182 kb on + strand, within PP_0819at 958.279 kb on + strand, within PP_0819at 958.357 kb on - strandat 958.357 kb on - strandat 958.508 kb on - strandat 958.741 kb on + strand, within PP_0820at 958.742 kb on - strand, within PP_0820at 958.774 kb on + strand, within PP_0820at 958.774 kb on + strand, within PP_0820at 958.775 kb on - strand, within PP_0820at 958.888 kb on + strand, within PP_0820at 958.888 kb on + strand, within PP_0820at 958.889 kb on - strand, within PP_0820at 958.889 kb on - strand, within PP_0820at 958.889 kb on - strand, within PP_0820at 958.889 kb on - strand, within PP_0820at 958.889 kb on - strand, within PP_0820at 958.889 kb on - strand, within PP_0820at 959.077 kb on + strandat 959.077 kb on + strandat 959.078 kb on - strandat 959.078 kb on - strandat 959.102 kb on + strandat 959.102 kb on + strandat 959.103 kb on - strandat 959.115 kb on + strandat 959.165 kb on + strandat 959.165 kb on + strandat 959.165 kb on + strandat 959.165 kb on + strandat 959.165 kb on + strandat 959.165 kb on + strandat 959.166 kb on - strandat 959.166 kb on - strandat 959.216 kb on + strandat 959.216 kb on + strandat 959.232 kb on + strandat 959.232 kb on + strandat 959.232 kb on + strandat 959.232 kb on + strandat 959.282 kb on + strand, within PP_5460at 959.283 kb on - strand, within PP_5460at 959.338 kb on - strand, within PP_5460at 959.430 kb on + strand, within PP_5460at 959.431 kb on - strand, within PP_5460at 959.481 kb on + strand, within PP_5460at 959.482 kb on - strand, within PP_5460at 959.482 kb on - strand, within PP_5460at 959.520 kb on + strand, within PP_5460at 959.521 kb on - strandat 959.655 kb on + strand, within PP_5460at 959.655 kb on + strand, within PP_5460at 959.655 kb on + strand, within PP_5460at 959.656 kb on - strand, within PP_5460at 959.752 kb on + strandat 959.769 kb on - strandat 959.769 kb on - strandat 959.769 kb on - strandat 959.822 kb on + strand, within PP_0821at 960.006 kb on - strand, within PP_0821at 960.034 kb on - strand, within PP_0821at 960.034 kb on - strand, within PP_0821at 960.034 kb on - strand, within PP_0821at 960.034 kb on - strand, within PP_0821

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Per-strain Table

Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 1
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957,596 + PP_0818 0.81 -0.2
957,596 + PP_0818 0.81 +0.2
957,629 + PP_0818 0.84 +0.1
957,630 - PP_0818 0.84 -0.5
957,630 - PP_0818 0.84 -0.2
957,646 + PP_0818 0.86 +0.1
957,646 + PP_0818 0.86 -0.0
957,646 + PP_0818 0.86 +0.0
957,646 + PP_0818 0.86 +0.4
957,646 + PP_0818 0.86 -0.1
957,646 + PP_0818 0.86 -0.2
957,646 + PP_0818 0.86 +0.3
957,653 + PP_0818 0.86 -0.1
957,654 - PP_0818 0.86 +0.3
957,654 - PP_0818 0.86 +0.2
957,660 + PP_0818 0.87 -0.3
957,712 + -0.2
957,744 - -0.1
957,762 + -0.0
957,762 + +1.1
957,763 - -0.7
957,763 - +0.1
957,791 + -0.2
957,893 - -0.1
957,979 + -0.4
957,979 + -0.1
957,980 - -0.4
957,980 - -0.3
957,980 - -0.2
957,981 + -0.2
957,982 - +0.3
957,982 - -0.0
958,064 + PP_0819 0.24 +0.4
958,065 - PP_0819 0.25 -0.0
958,085 + PP_0819 0.30 -0.3
958,086 - PP_0819 0.30 -0.2
958,086 - PP_0819 0.30 -0.5
958,086 - PP_0819 0.30 -0.4
958,086 - PP_0819 0.30 +0.5
958,182 + PP_0819 0.55 -0.4
958,279 + PP_0819 0.80 -0.4
958,357 - -0.6
958,357 - -0.1
958,508 - -1.2
958,741 + PP_0820 0.35 -0.1
958,742 - PP_0820 0.35 -0.7
958,774 + PP_0820 0.40 +0.4
958,774 + PP_0820 0.40 -0.5
958,775 - PP_0820 0.41 +0.1
958,888 + PP_0820 0.61 -0.4
958,888 + PP_0820 0.61 +0.2
958,889 - PP_0820 0.61 +0.3
958,889 - PP_0820 0.61 -0.2
958,889 - PP_0820 0.61 -0.2
958,889 - PP_0820 0.61 -0.1
958,889 - PP_0820 0.61 -0.8
958,889 - PP_0820 0.61 -0.7
959,077 + +0.9
959,077 + -0.1
959,078 - -0.3
959,078 - -0.2
959,102 + -1.1
959,102 + -0.3
959,103 - -0.5
959,115 + +0.0
959,165 + -0.0
959,165 + -1.1
959,165 + -0.6
959,165 + +0.0
959,165 + -0.5
959,165 + +0.5
959,166 - -0.3
959,166 - +1.1
959,216 + -0.1
959,216 + +0.2
959,232 + -0.1
959,232 + -0.1
959,232 + -0.2
959,232 + +0.4
959,282 + PP_5460 0.16 -0.5
959,283 - PP_5460 0.16 +0.1
959,338 - PP_5460 0.26 +0.1
959,430 + PP_5460 0.43 +0.3
959,431 - PP_5460 0.44 +0.2
959,481 + PP_5460 0.53 +0.8
959,482 - PP_5460 0.53 -0.1
959,482 - PP_5460 0.53 +0.2
959,520 + PP_5460 0.60 -0.2
959,521 - +0.3
959,655 + PP_5460 0.85 +0.1
959,655 + PP_5460 0.85 -0.4
959,655 + PP_5460 0.85 -0.0
959,656 - PP_5460 0.85 +0.0
959,752 + -0.1
959,769 - -0.1
959,769 - -0.3
959,769 - +0.4
959,822 + PP_0821 0.22 -0.3
960,006 - PP_0821 0.67 -0.2
960,034 - PP_0821 0.73 +0.2
960,034 - PP_0821 0.73 -0.2
960,034 - PP_0821 0.73 -0.4
960,034 - PP_0821 0.73 -0.3

Or see this region's nucleotide sequence