Strain Fitness in Pseudomonas putida KT2440 around PP_4576

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
5,194,845	-				+0.5
5,194,912	+				-0.2
5,194,930	+				-0.1
5,194,930	+				-0.3
5,195,112	+		PP_4575	0.22	-0.5
5,195,112	+		PP_4575	0.22	+0.6
5,195,159	-		PP_4575	0.27	+0.1
5,195,159	-		PP_4575	0.27	-0.1
5,195,159	-		PP_4575	0.27	-0.5
5,195,427	+		PP_4575	0.56	+0.3
5,195,428	-		PP_4575	0.56	-0.3
5,195,428	-		PP_4575	0.56	-0.4
5,195,488	+		PP_4575	0.63	+0.2
5,195,998	-		PP_4576	0.25	+1.0
5,196,022	+		PP_4576	0.29	+0.4
5,196,235	-		PP_4576	0.59	-0.6
5,196,235	-		PP_4576	0.59	-0.2
5,196,355	+		PP_4576	0.77	-0.5
5,196,355	+		PP_4576	0.77	-0.6
5,196,355	+				+1.0
5,196,355	+		PP_4576	0.77	-0.5
5,196,356	-		PP_4576	0.77	+0.4
5,196,356	-		PP_4576	0.77	-0.3
5,196,356	-		PP_4576	0.77	-0.1
5,196,358	+		PP_4576	0.77	+0.1
5,196,358	+		PP_4576	0.77	-0.8
5,196,358	+		PP_4576	0.77	-0.2
5,196,358	+		PP_4576	0.77	-0.1
5,196,358	+		PP_4576	0.77	+0.1
5,196,358	+		PP_4576	0.77	-0.3
5,196,358	+		PP_4576	0.77	+0.1
5,196,359	-		PP_4576	0.77	-0.1
5,196,359	-		PP_4576	0.77	-0.9
5,196,361	-		PP_4576	0.78	-0.2
5,196,361	-		PP_4576	0.78	+0.7
5,196,361	-		PP_4576	0.78	+0.3
5,196,361	-		PP_4576	0.78	-0.9
5,196,361	-		PP_4576	0.78	+0.0
5,196,361	-		PP_4576	0.78	-0.6
5,196,447	+				-0.3
5,196,468	-				-0.3
5,196,468	-				+0.1
5,196,740	+		PP_4577	0.29	+0.4
5,196,740	+		PP_4577	0.29	-0.6
5,196,740	+		PP_4577	0.29	+0.3
5,196,741	-		PP_4577	0.30	-0.9
5,196,914	+		PP_4577	0.52	+0.3
5,196,914	+		PP_4577	0.52	-0.5
5,196,914	+		PP_4577	0.52	+0.3
5,196,938	+		PP_4577	0.55	+0.3
5,196,938	+		PP_4577	0.55	-1.2
5,196,939	-		PP_4577	0.55	-0.9
5,196,939	-		PP_4577	0.55	-0.1
5,196,939	-		PP_4577	0.55	-0.2
5,196,939	-		PP_4577	0.55	+1.0
5,196,992	+		PP_4577	0.62	-0.6
5,197,083	+		PP_4577	0.74	+0.0
5,197,178	+		PP_4577	0.86	-0.9
5,197,178	+		PP_4577	0.86	+0.2
5,197,179	-		PP_4577	0.86	-0.7
5,197,179	-		PP_4577	0.86	-0.6
5,197,246	+				-0.2
5,197,247	-				+0.1
5,197,287	+				-0.0
5,197,287	+				-0.0
5,197,287	+				-0.7
5,197,287	+				-1.4
5,197,288	-				-0.5
5,197,288	-				+0.2
5,197,288	-				-0.5
5,197,293	+				-0.1
5,197,293	+				-0.3
5,197,293	+				-0.4
5,197,294	-				-0.4
5,197,294	-				-0.4
5,197,364	+				+2.3
5,197,469	+		PP_4578	0.13	-0.5
5,197,470	-		PP_4578	0.13	+0.7