Strain Fitness in Pseudomonas putida KT2440 around PP_4494

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
5,107,267	+		PP_4493	0.72	+0.2
5,107,267	+		PP_4493	0.72	+0.1
5,107,267	+		PP_4493	0.72	-0.1
5,107,267	+		PP_4493	0.72	-0.3
5,107,310	-		PP_4493	0.73	+0.0
5,107,338	+		PP_4493	0.74	+0.1
5,107,339	-		PP_4493	0.74	-0.2
5,107,339	-		PP_4493	0.74	+0.6
5,107,475	-		PP_4493	0.79	+0.0
5,107,606	+		PP_4493	0.83	-0.3
5,107,607	-		PP_4493	0.83	+0.5
5,107,607	-		PP_4493	0.83	-0.3
5,107,821	-				-0.8
5,107,972	+				+0.4
5,107,996	-				-0.0
5,108,012	-				-0.0
5,108,131	-				+0.2
5,108,137	+				-0.7
5,108,150	-				+0.2
5,108,191	+				+0.2
5,108,192	-				-0.7
5,108,192	-				-0.2
5,108,194	-				+1.9
5,108,260	-				-0.3
5,108,337	-				+0.4
5,108,337	-				+0.7
5,108,337	-				+0.0
5,108,391	+		PP_4494	0.14	+0.1
5,108,391	+		PP_4494	0.14	+0.3
5,108,392	-		PP_4494	0.14	-0.2
5,108,392	-		PP_4494	0.14	-0.1
5,108,448	+		PP_4494	0.21	+0.5
5,108,449	-		PP_4494	0.21	+0.2
5,108,449	-		PP_4494	0.21	-0.3
5,108,475	-		PP_4494	0.24	-0.1
5,108,537	+		PP_4494	0.31	-0.3
5,108,538	-		PP_4494	0.31	-0.0
5,108,550	+		PP_4494	0.32	-0.2
5,108,591	-		PP_4494	0.37	-0.0
5,108,591	-		PP_4494	0.37	-0.3
5,108,621	+		PP_4494	0.40	+0.5
5,108,621	+		PP_4494	0.40	+1.0
5,108,962	-		PP_4494	0.79	-0.2
5,108,997	+		PP_4494	0.83	-0.0
5,108,998	-		PP_4494	0.83	-0.3
5,108,998	-		PP_4494	0.83	+0.0
5,109,032	+		PP_4494	0.87	-0.2
5,109,032	+		PP_4494	0.87	+0.0
5,109,032	+		PP_4494	0.87	-0.7
5,109,032	+		PP_4494	0.87	-0.4
5,109,032	+		PP_4494	0.87	+0.2
5,109,032	+		PP_4494	0.87	+0.1
5,109,032	+		PP_4494	0.87	-0.1
5,109,032	+		PP_4494	0.87	+0.2
5,109,033	-		PP_4494	0.87	-0.1
5,109,033	-		PP_4494	0.87	+0.3
5,109,033	-		PP_4494	0.87	-0.0
5,109,068	+				-0.0
5,109,068	+				-0.2
5,109,068	+				+1.9
5,109,071	+				-0.4
5,109,071	+				-0.5
5,109,072	-				-0.0
5,109,080	+				+0.1
5,109,081	-				-0.4
5,109,191	+				+0.2
5,109,272	-				-0.5
5,109,288	+				-0.0
5,109,288	+				-0.1
5,109,288	+				+0.6
5,109,288	+				-0.0
5,109,289	-				+0.4
5,109,289	-				-0.3
5,109,289	-				+0.2
5,109,348	+				+0.5
5,109,348	+				-0.5
5,109,348	+				+0.1
5,109,348	+				+0.3
5,109,348	+				-0.1
5,109,348	+				+0.5
5,109,349	-				+0.2
5,109,349	-				+0.3
5,109,349	-				+0.1
5,109,349	-				-0.1
5,109,349	-				-0.4
5,109,349	-				+0.1
5,109,349	-				-0.4
5,109,387	+				+0.4
5,109,387	+				+0.5
5,109,388	-				+0.1
5,109,388	-				-0.4
5,109,388	-				-0.1
5,109,388	-				-0.4
5,109,388	-				-0.4
5,109,459	+		PP_4495	0.12	+0.1
5,109,459	+		PP_4495	0.12	-0.1
5,109,459	+		PP_4495	0.12	+0.0
5,109,459	+		PP_4495	0.12	+0.0
5,109,459	+		PP_4495	0.12	-0.5
5,109,459	+		PP_4495	0.12	-0.2
5,109,460	-		PP_4495	0.12	-0.3
5,109,460	-		PP_4495	0.12	-0.6
5,109,460	-		PP_4495	0.12	-0.2
5,109,460	-		PP_4495	0.12	-0.1
5,109,460	-		PP_4495	0.12	-0.6
5,109,460	-		PP_4495	0.12	-0.0
5,109,543	+		PP_4495	0.18	-0.3
5,109,543	+		PP_4495	0.18	+0.1
5,109,625	-		PP_4495	0.24	+0.5
5,109,765	+		PP_4495	0.34	-0.1
5,109,780	+		PP_4495	0.35	-0.3
5,109,781	-		PP_4495	0.35	-0.1
5,109,879	+		PP_4495	0.42	-0.1
5,109,978	+		PP_4495	0.49	-0.3
5,109,979	-		PP_4495	0.49	-0.1