Strain Fitness in Pseudomonas putida KT2440 around PP_0080

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0079 and PP_0080 are separated by 121 nucleotidesPP_0080 and PP_0081 are separated by 13 nucleotidesPP_0081 and PP_0082 are separated by 120 nucleotides PP_0079: PP_0079 - putative transcriptional regulator, at 89,426 to 90,325 _0079 PP_0080: PP_0080 - conserved protein of unknown function, at 90,447 to 91,064 _0080 PP_0081: PP_0081 - conserved protein of unknown function, at 91,078 to 91,449 _0081 PP_0082: PP_0082 - tryptophan synthase alpha chain, at 91,570 to 92,379 _0082 Position (kb) 90 91 92Strain fitness (log2 ratio) -2 -1 0 1at 89.470 kb on - strandat 89.470 kb on - strandat 89.680 kb on + strand, within PP_0079at 89.789 kb on + strand, within PP_0079at 89.790 kb on - strand, within PP_0079at 90.103 kb on + strand, within PP_0079at 90.103 kb on + strand, within PP_0079at 90.103 kb on + strand, within PP_0079at 90.104 kb on - strand, within PP_0079at 90.104 kb on - strand, within PP_0079at 90.107 kb on + strand, within PP_0079at 90.107 kb on + strand, within PP_0079at 90.107 kb on + strand, within PP_0079at 90.107 kb on + strand, within PP_0079at 90.108 kb on - strand, within PP_0079at 90.108 kb on - strand, within PP_0079at 90.108 kb on - strand, within PP_0079at 90.108 kb on - strand, within PP_0079at 90.108 kb on - strand, within PP_0079at 90.108 kb on - strand, within PP_0079at 90.109 kb on + strand, within PP_0079at 90.109 kb on + strand, within PP_0079at 90.109 kb on + strand, within PP_0079at 90.109 kb on + strand, within PP_0079at 90.109 kb on + strand, within PP_0079at 90.110 kb on - strand, within PP_0079at 90.110 kb on - strand, within PP_0079at 90.239 kb on + strandat 90.239 kb on + strandat 90.239 kb on + strandat 90.240 kb on - strandat 90.242 kb on + strandat 90.242 kb on + strandat 90.242 kb on + strandat 90.242 kb on + strandat 90.242 kb on + strandat 90.242 kb on + strandat 90.242 kb on + strandat 90.243 kb on - strandat 90.243 kb on - strandat 90.243 kb on - strandat 90.243 kb on - strandat 90.243 kb on - strandat 90.246 kb on + strandat 90.246 kb on + strandat 90.247 kb on - strandat 90.252 kb on + strandat 90.252 kb on + strandat 90.253 kb on - strandat 90.448 kb on + strandat 90.448 kb on + strandat 90.448 kb on + strandat 90.573 kb on - strand, within PP_0080at 90.595 kb on - strand, within PP_0080at 90.697 kb on + strand, within PP_0080at 90.698 kb on - strand, within PP_0080at 90.698 kb on - strand, within PP_0080at 90.698 kb on - strand, within PP_0080at 90.750 kb on + strand, within PP_0080at 90.750 kb on + strand, within PP_0080at 90.751 kb on - strand, within PP_0080at 90.751 kb on - strand, within PP_0080at 90.753 kb on - strand, within PP_0080at 90.753 kb on - strand, within PP_0080at 90.831 kb on + strand, within PP_0080at 91.113 kb on + strandat 91.114 kb on - strandat 91.136 kb on - strand, within PP_0081at 91.150 kb on + strand, within PP_0081at 91.151 kb on - strand, within PP_0081at 91.151 kb on - strand, within PP_0081at 91.153 kb on + strand, within PP_0081at 91.154 kb on - strand, within PP_0081at 91.346 kb on + strand, within PP_0081at 91.347 kb on - strand, within PP_0081at 91.347 kb on - strand, within PP_0081at 91.347 kb on - strand, within PP_0081at 91.347 kb on - strand, within PP_0081at 91.447 kb on + strandat 91.447 kb on + strandat 91.448 kb on - strandat 91.494 kb on + strandat 91.535 kb on - strandat 91.547 kb on - strandat 91.621 kb on + strandat 91.622 kb on - strandat 91.853 kb on + strand, within PP_0082at 91.853 kb on + strand, within PP_0082at 91.853 kb on + strand, within PP_0082at 91.854 kb on - strand, within PP_0082at 91.856 kb on + strand, within PP_0082

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Per-strain Table

Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
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89,470 - -0.3
89,470 - -0.3
89,680 + PP_0079 0.28 -0.2
89,789 + PP_0079 0.40 +0.1
89,790 - PP_0079 0.40 -0.0
90,103 + PP_0079 0.75 +0.0
90,103 + PP_0079 0.75 -0.6
90,103 + PP_0079 0.75 -0.8
90,104 - PP_0079 0.75 -0.3
90,104 - PP_0079 0.75 -0.2
90,107 + PP_0079 0.76 -0.3
90,107 + PP_0079 0.76 -0.2
90,107 + PP_0079 0.76 -0.9
90,107 + PP_0079 0.76 -0.5
90,108 - PP_0079 0.76 +0.8
90,108 - PP_0079 0.76 +0.2
90,108 - PP_0079 0.76 -0.3
90,108 - PP_0079 0.76 -0.2
90,108 - PP_0079 0.76 +0.1
90,108 - PP_0079 0.76 -0.6
90,109 + PP_0079 0.76 -0.4
90,109 + PP_0079 0.76 -0.3
90,109 + PP_0079 0.76 -0.2
90,109 + PP_0079 0.76 -0.3
90,109 + PP_0079 0.76 -0.1
90,110 - PP_0079 0.76 -0.3
90,110 - PP_0079 0.76 -0.1
90,239 + +0.5
90,239 + -0.3
90,239 + +0.1
90,240 - -0.1
90,242 + -0.4
90,242 + -0.2
90,242 + -0.3
90,242 + -0.5
90,242 + +0.3
90,242 + -0.4
90,242 + -0.1
90,243 - -0.9
90,243 - +0.1
90,243 - -0.2
90,243 - +0.4
90,243 - +0.0
90,246 + +0.1
90,246 + -0.1
90,247 - +0.4
90,252 + -0.2
90,252 + -0.4
90,253 - -0.3
90,448 + +0.3
90,448 + -0.1
90,448 + -0.4
90,573 - PP_0080 0.20 +0.5
90,595 - PP_0080 0.24 +0.0
90,697 + PP_0080 0.40 +0.1
90,698 - PP_0080 0.41 +0.8
90,698 - PP_0080 0.41 -0.1
90,698 - PP_0080 0.41 +0.4
90,750 + PP_0080 0.49 -0.0
90,750 + PP_0080 0.49 +0.7
90,751 - PP_0080 0.49 -0.9
90,751 - PP_0080 0.49 +0.4
90,753 - PP_0080 0.50 +0.5
90,753 - PP_0080 0.50 +0.4
90,831 + PP_0080 0.62 -0.0
91,113 + +0.1
91,114 - -0.4
91,136 - PP_0081 0.16 -0.1
91,150 + PP_0081 0.19 +0.0
91,151 - PP_0081 0.20 -0.2
91,151 - PP_0081 0.20 -0.3
91,153 + PP_0081 0.20 -0.7
91,154 - PP_0081 0.20 +0.4
91,346 + PP_0081 0.72 -0.6
91,347 - PP_0081 0.72 -0.1
91,347 - PP_0081 0.72 -0.4
91,347 - PP_0081 0.72 +0.2
91,347 - PP_0081 0.72 -0.5
91,447 + +0.3
91,447 + +0.6
91,448 - +0.2
91,494 + +0.2
91,535 - -0.5
91,547 - +0.1
91,621 + -0.4
91,622 - -0.4
91,853 + PP_0082 0.35 -1.7
91,853 + PP_0082 0.35 +0.2
91,853 + PP_0082 0.35 -2.3
91,854 - PP_0082 0.35 -0.8
91,856 + PP_0082 0.35 -0.8

Or see this region's nucleotide sequence