Strain Fitness in Pseudomonas putida KT2440 around PP_3668

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
4,166,703	+		PP_3667	0.36	-0.1
4,166,704	-				-0.6
4,166,704	-		PP_3667	0.36	-0.0
4,166,704	-		PP_3667	0.36	+0.3
4,166,704	-		PP_3667	0.36	+0.2
4,166,901	+		PP_3667	0.53	-0.4
4,166,977	+		PP_3667	0.59	-0.1
4,166,978	-		PP_3667	0.59	-0.3
4,166,978	-		PP_3667	0.59	-1.0
4,167,014	-		PP_3667	0.62	-0.1
4,167,014	-		PP_3667	0.62	-0.4
4,167,057	+		PP_3667	0.65	+0.1
4,167,102	+		PP_3667	0.69	-0.2
4,167,103	-		PP_3667	0.69	+0.8
4,167,103	-		PP_3667	0.69	+0.1
4,167,103	-		PP_3667	0.69	+0.6
4,167,156	+		PP_3667	0.74	-0.3
4,167,157	-		PP_3667	0.74	-0.8
4,167,279	+		PP_3667	0.84	+0.5
4,167,279	+		PP_3667	0.84	+0.6
4,167,300	+		PP_3667	0.85	+0.4
4,167,315	+		PP_3667	0.87	-0.0
4,167,316	-		PP_3667	0.87	-0.5
4,167,318	+		PP_3667	0.87	+0.5
4,167,374	-				-0.1
4,167,375	+				-0.5
4,167,375	+				-0.7
4,167,375	+				-0.1
4,167,375	+				-0.5
4,167,376	-				-0.2
4,167,376	-				+0.5
4,167,376	-				-0.7
4,167,376	-				-0.1
4,167,376	-				+0.6
4,167,376	-				+0.8
4,167,376	-				-0.0
4,167,376	-				+0.0
4,167,376	-				-0.0
4,167,405	+				-0.2
4,167,561	+				-0.4
4,167,562	-				-0.2
4,167,562	-				-0.3
4,167,565	+				-0.1
4,167,566	-				-0.1
4,167,639	-				-0.4
4,167,639	-				-0.2
4,167,728	+				-0.7
4,167,728	+				-0.6
4,167,728	+				-0.1
4,167,728	+				+0.0
4,167,729	-				+0.0
4,167,729	-				+1.5
4,167,729	-				-0.1
4,167,729	-				-0.3
4,167,729	-				-0.4
4,167,779	-				+0.3
4,167,873	+		PP_3668	0.10	-0.4
4,168,058	+		PP_3668	0.19	-0.3
4,168,059	-		PP_3668	0.19	-0.2
4,168,168	+		PP_3668	0.24	+0.2
4,168,169	-		PP_3668	0.24	+0.3
4,168,181	+		PP_3668	0.24	+0.4
4,168,181	+		PP_3668	0.24	-0.4
4,168,182	-		PP_3668	0.24	-0.2
4,168,608	-		PP_3668	0.43	-0.4
4,168,608	-		PP_3668	0.43	+0.7
4,168,676	+		PP_3668	0.46	-0.5
4,168,845	+		PP_3668	0.54	-0.0
4,168,846	-		PP_3668	0.54	+0.7
4,168,914	+		PP_3668	0.57	+0.7
4,168,915	-		PP_3668	0.57	-0.0
4,169,162	+		PP_3668	0.68	+0.4
4,169,162	+		PP_3668	0.68	+0.1
4,169,163	-		PP_3668	0.68	+0.4
4,169,163	-		PP_3668	0.68	-0.4
4,169,163	-		PP_3668	0.68	-0.1
4,169,163	-		PP_3668	0.68	+0.5
4,169,163	-		PP_3668	0.68	+1.0
4,169,163	-		PP_3668	0.68	+0.0
4,169,163	-		PP_3668	0.68	-0.3
4,169,163	-		PP_3668	0.68	+0.1
4,169,317	-		PP_3668	0.74	-0.0
4,169,351	+		PP_3668	0.76	-0.1
4,169,351	+		PP_3668	0.76	-0.6
4,169,351	+		PP_3668	0.76	-0.4
4,169,351	+		PP_3668	0.76	-0.2
4,169,606	+				+1.0
4,169,619	+		PP_3668	0.88	-0.0
4,169,900	+				+0.0
4,169,932	+				-0.0
4,169,932	+				+1.8
4,169,968	+				-0.1
4,169,968	+				+0.3
4,169,969	-				-1.9
4,169,971	+				+0.3
4,169,972	-				-0.9
4,169,987	+				-0.1
4,169,987	+				+0.4
4,169,987	+				-0.5
4,169,987	+				-0.1
4,170,110	+				+0.7
4,170,110	+				+0.7
4,170,110	+				-0.2
4,170,110	+				-0.5
4,170,111	-				-0.4
4,170,113	+				+0.3
4,170,113	+				-0.2
4,170,113	+				+0.7
4,170,114	-				+0.3
4,170,114	-				-0.3
4,170,114	-				+0.3
4,170,114	-				-0.1
4,170,114	-				-0.8
4,170,114	-				+0.4
4,170,114	-				+0.1
4,170,114	-				+0.6
4,170,136	-				+0.3
4,170,136	-				-0.4
4,170,209	+				+0.8
4,170,209	+				-0.0
4,170,209	+				+0.1
4,170,209	+				-0.5
4,170,209	+				-0.2
4,170,209	+				-0.2
4,170,209	+				-0.6
4,170,209	+				+0.1
4,170,210	-				+0.4
4,170,210	-				-0.3
4,170,210	-				-0.1
4,170,210	-				-0.2
4,170,210	-				-0.0
4,170,210	-				+0.1
4,170,241	+				+0.3
4,170,380	+		PP_3669	0.11	-0.4
4,170,674	-		PP_3669	0.44	-0.3
4,170,674	-		PP_3669	0.44	+0.2
4,170,674	-		PP_3669	0.44	+0.2
4,170,733	+		PP_3669	0.50	-1.2
4,170,733	+		PP_3669	0.50	+0.2
4,170,734	-		PP_3669	0.50	-0.2
4,170,744	+		PP_3669	0.51	-0.1
4,170,744	+		PP_3669	0.51	+0.3
4,170,744	+		PP_3669	0.51	-0.4
4,170,744	+		PP_3669	0.51	-0.1
4,170,744	+		PP_3669	0.51	+0.2
4,170,744	+		PP_3669	0.51	+0.3
4,170,745	-		PP_3669	0.51	+1.2
4,170,745	-		PP_3669	0.51	+0.2
4,170,745	-		PP_3669	0.51	+0.6
4,170,746	+		PP_3669	0.52	+0.1
4,170,747	-		PP_3669	0.52	+0.6
4,170,747	-		PP_3669	0.52	-0.7
4,170,747	-		PP_3669	0.52	+0.2
4,170,793	+		PP_3669	0.57	-0.2
4,170,793	+		PP_3669	0.57	-0.2
4,170,794	-		PP_3669	0.57	+0.0
4,170,794	-		PP_3669	0.57	-0.2
4,170,794	-		PP_3669	0.57	-0.2
4,170,794	-		PP_3669	0.57	+0.1
4,170,794	-		PP_3669	0.57	+0.1
4,170,794	-		PP_3669	0.57	+0.1
4,170,794	-		PP_3669	0.57	+0.4
4,170,799	+		PP_3669	0.57	+0.1
4,170,850	-		PP_3669	0.63	-0.8