Strain Fitness in Pseudomonas putida KT2440 around PP_3300

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
3,732,433	+		PP_3299	0.45	-0.6
3,732,553	-		PP_3299	0.53	+0.1
3,732,669	+		PP_3299	0.61	-0.1
3,732,669	+		PP_3299	0.61	-0.2
3,732,669	+		PP_3299	0.61	+0.0
3,732,669	+		PP_3299	0.61	+0.5
3,732,670	-		PP_3299	0.61	-0.8
3,732,670	-		PP_3299	0.61	+0.4
3,732,670	-		PP_3299	0.61	-0.4
3,732,736	-		PP_3299	0.66	-0.3
3,732,816	+		PP_3299	0.71	-0.1
3,732,816	+		PP_3299	0.71	-0.2
3,732,816	+		PP_3299	0.71	-0.0
3,732,816	+		PP_3299	0.71	-0.5
3,732,817	-		PP_3299	0.71	-0.1
3,732,837	+		PP_3299	0.72	-0.5
3,732,838	-		PP_3299	0.72	+0.4
3,732,838	-		PP_3299	0.72	-0.0
3,732,972	-		PP_3299	0.82	+0.2
3,733,122	+				-1.3
3,733,123	-				+0.1
3,733,153	+				-0.3
3,733,155	+				-0.6
3,733,269	-				+0.0
3,733,318	-				-0.5
3,733,409	-		PP_3300	0.16	-0.3
3,733,414	+		PP_3300	0.17	+0.6
3,733,468	+		PP_3300	0.25	-0.3
3,733,468	+		PP_3300	0.25	+0.0
3,733,474	+		PP_3300	0.26	-0.7
3,733,475	-		PP_3300	0.27	+0.4
3,733,475	-		PP_3300	0.27	-0.4
3,733,601	+		PP_3300	0.47	+0.0
3,733,601	+		PP_3300	0.47	-0.3
3,733,602	-		PP_3300	0.47	-0.0
3,733,658	-		PP_3300	0.56	+0.4
3,733,658	-		PP_3300	0.56	-0.0
3,733,658	-		PP_3300	0.56	-0.2
3,733,664	+		PP_3300	0.57	-0.4
3,733,665	-		PP_3300	0.57	+0.6
3,733,719	+		PP_3300	0.66	-0.6
3,733,719	+		PP_3300	0.66	-0.4
3,733,719	+		PP_3300	0.66	-0.5
3,733,720	-		PP_3300	0.66	-0.5
3,733,725	+		PP_3300	0.67	+0.7
3,733,725	+		PP_3300	0.67	-0.1
3,733,726	-		PP_3300	0.67	-0.0
3,733,726	-		PP_3300	0.67	+0.3
3,733,726	-		PP_3300	0.67	+0.0
3,733,831	+		PP_3300	0.84	-0.4
3,733,832	-		PP_3300	0.85	-0.4
3,733,835	+		PP_3300	0.85	-0.5
3,733,835	+		PP_3300	0.85	-0.9
3,733,836	-		PP_3300	0.85	+0.1
3,733,836	-		PP_3300	0.85	-0.1
3,733,836	-		PP_3300	0.85	-0.3
3,733,924	+				-0.2
3,733,925	-				+0.3
3,733,977	+				-0.1
3,733,978	-				-0.3
3,733,978	-				-0.1
3,733,979	+				-0.1
3,734,026	+				+0.4
3,734,038	+				-0.4
3,734,039	-				-0.5
3,734,125	+		PP_3301	0.11	-0.3
3,734,126	-		PP_3301	0.11	-0.3
3,734,165	-		PP_3301	0.15	-0.5
3,734,323	+		PP_3301	0.29	+0.6
3,734,415	+		PP_3301	0.37	-0.3
3,734,513	-		PP_3301	0.46	-0.0
3,734,593	+		PP_3301	0.53	+0.0
3,734,594	-		PP_3301	0.53	-0.1
3,734,594	-		PP_3301	0.53	-0.7
3,734,632	-		PP_3301	0.57	+0.3
3,734,632	-		PP_3301	0.57	-0.1
3,734,683	+		PP_3301	0.62	-0.7
3,734,773	-		PP_3301	0.70	+0.7
3,734,819	+		PP_3301	0.74	-0.0
3,734,819	+		PP_3301	0.74	-0.1
3,734,819	+		PP_3301	0.74	+0.4
3,734,866	+		PP_3301	0.78	+0.2